PMID- 27079798
OWN - NLM
STAT- MEDLINE
DCOM- 20170309
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Apr 15
TI  - Lysine fatty acylation promotes lysosomal targeting of TNF-alpha.
PG  - 24371
LID - 10.1038/srep24371 [doi]
LID - 24371
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a proinflammation cytokine secreted by various 
      cells. Understanding its secretive pathway is important to understand the 
      biological functions of TNF-alpha and diseases associated with TNF-alpha. TNF-alpha is one of 
      the first proteins known be modified by lysine fatty acylation (e.g. 
      myristoylation). We previously demonstrated that SIRT6, a member of the mammalian 
      sirtuin family of enzymes, can remove the fatty acyl modification on TNF-alpha and 
      promote its secretion. However, the mechanistic details about how lysine fatty 
      acylation regulates TNF-alpha secretion have been unknown. Here we present 
      experimental data supporting that lysine fatty acylation promotes lysosomal 
      targeting of TNF-alpha. The result is an important first step toward understanding 
      the biological functions of lysine fatty acylation.
FAU - Jiang, Hong
AU  - Jiang H
AD  - Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 
      14853, USA.
FAU - Zhang, Xiaoyu
AU  - Zhang X
AD  - Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 
      14853, USA.
FAU - Lin, Hening
AU  - Lin H
AD  - Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 
      14853, USA.
AD  - Howard Hughes Medical Institute, Cornell University, Ithaca, NY 14853, USA.
LA  - eng
GR  - GM086703/GM/NIGMS NIH HHS/United States
GR  - S10OD018516/OD/NIH HHS/United States
GR  - DK107868/DK/NIDDK NIH HHS/United States
GR  - Howard Hughes Medical Institute/United States
GR  - S10 OD018516/OD/NIH HHS/United States
GR  - R01 GM086703/GM/NIGMS NIH HHS/United States
GR  - R01 DK107868/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20160415
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Acylation
MH  - Cell Line
MH  - Cytoplasmic Vesicles/metabolism
MH  - Humans
MH  - Lysine/*metabolism
MH  - Lysosomes/*metabolism
MH  - Organelles/metabolism
MH  - Protein Transport
MH  - Proteolysis
MH  - Tumor Necrosis Factor-alpha/*metabolism
PMC - PMC4832147
EDAT- 2016/04/16 06:00
MHDA- 2017/03/10 06:00
PMCR- 2016/04/15
CRDT- 2016/04/16 06:00
PHST- 2015/12/07 00:00 [received]
PHST- 2016/03/21 00:00 [accepted]
PHST- 2016/04/16 06:00 [entrez]
PHST- 2016/04/16 06:00 [pubmed]
PHST- 2017/03/10 06:00 [medline]
PHST- 2016/04/15 00:00 [pmc-release]
AID - srep24371 [pii]
AID - 10.1038/srep24371 [doi]
PST - epublish
SO  - Sci Rep. 2016 Apr 15;6:24371. doi: 10.1038/srep24371.