PMID- 27082014
OWN - NLM
STAT- MEDLINE
DCOM- 20170228
LR  - 20191210
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 37
IP  - 6
DP  - 2016 Jun
TI  - Plumbagin exerts protective effects in nucleus pulposus cells by attenuating 
      hydrogen peroxide-induced oxidative stress, inflammation and apoptosis through 
      NF-kappaB and Nrf-2.
PG  - 1669-76
LID - 10.3892/ijmm.2016.2564 [doi]
AB  - Plumbagin, one of the constituents responsible for the various biological 
      activities of Plumbago zeylanica has been demonstrated to possess antioxidant 
      activity, which may inhibit lipid peroxidation in a dose- and time-dependent 
      manner. In the present study, we aimed to examine the protective effects of 
      plumbagin as well as the underlying mechansim through which plumbagin attenuates 
      hydrogen peroxide (H2O2)-induced oxidative stress in nucleus pulposus 
      cells (NPCs). For this purpose, the NPCs were incubated with fresh medium 
      containing H2O2 (200 microM) at 37 C in a humidified 5% CO2 atmosphere for 6 h, and 
      cultured with various concentrations of plumbagin (0, 0.5, 1, 2, 5, 10 and 
      20 microM). Treatment with plumbagin significantly increased the viability of the 
      H2O2-exposed NPCs in a dose‑dependent manner. Moreover, plumbagin significantly 
      reduced the generation of reactive oxygen species, lipid peroxidation, as well as 
      the levels of tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta and IL-6 in 
      the H2O2‑exposed NPCs. Glutathione (GSH) content, as well as the activity of 
      catalase (CAT), superoxide dismutase (SOD) and glutathione peroxdiase (GSH-Px) 
      were increased. We found that the administration of plumbagin significantly 
      inhibited the activity of caspase-9 and -3, and downregulated NF-kappaB expression 
      and upregulated Nrf-2 expression in the H2O2-exposed NPCs. Taken together, these 
      findings suggest that plumbagin exerts neuroprotective effects in NPCs by 
      attenuating H2O2‑induced oxidative stress, inflammation and apoptosis through 
      mediating the expression of NF-kappaB and Nrf-2.
FAU - Chu, Hui
AU  - Chu H
AD  - Department of Orthopedics, The 100th Hospital of Chinese People's Liberation 
      Army, Suzhou, Jiangsu 215007, P.R. China.
FAU - Yu, Hang
AU  - Yu H
AD  - Department of Orthopedics, The 100th Hospital of Chinese People's Liberation 
      Army, Suzhou, Jiangsu 215007, P.R. China.
FAU - Ren, Ding
AU  - Ren D
AD  - Department of Orthopedics, The 100th Hospital of Chinese People's Liberation 
      Army, Suzhou, Jiangsu 215007, P.R. China.
FAU - Zhu, Kejun
AU  - Zhu K
AD  - Department of Orthopedics, The 100th Hospital of Chinese People's Liberation 
      Army, Suzhou, Jiangsu 215007, P.R. China.
FAU - Huang, Hong
AU  - Huang H
AD  - Department of Orthopedics, The 100th Hospital of Chinese People's Liberation 
      Army, Suzhou, Jiangsu 215007, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160414
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NF-kappa B)
RN  - 0 (Naphthoquinones)
RN  - 0 (Nfe2l2 protein, rat)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Reactive Oxygen Species)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - GAN16C9B8O (Glutathione)
RN  - YAS4TBQ4OQ (plumbagin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/*pharmacology
MH  - Apoptosis/drug effects
MH  - Catalase/genetics/metabolism
MH  - Chondrocytes/*drug effects/metabolism/pathology
MH  - Dose-Response Relationship, Drug
MH  - Gene Expression Regulation
MH  - Glutathione/metabolism
MH  - Glutathione Peroxidase/genetics/metabolism
MH  - Hydrogen Peroxide/*antagonists & inhibitors/pharmacology
MH  - Inflammation
MH  - Male
MH  - NF-E2-Related Factor 2/*agonists/genetics/metabolism
MH  - NF-kappa B/*agonists/genetics/metabolism
MH  - Naphthoquinones/*pharmacology
MH  - Nucleus Pulposus/drug effects/metabolism/pathology
MH  - Osteoblasts/*drug effects/metabolism/pathology
MH  - Oxidative Stress
MH  - Phosphatidylinositol 3-Kinases/genetics/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Primary Cell Culture
MH  - Proto-Oncogene Proteins c-akt/antagonists & inhibitors/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/antagonists & inhibitors/metabolism
MH  - Signal Transduction
MH  - Superoxide Dismutase/genetics/metabolism
EDAT- 2016/04/16 06:00
MHDA- 2017/03/01 06:00
CRDT- 2016/04/16 06:00
PHST- 2015/04/20 00:00 [received]
PHST- 2016/04/01 00:00 [accepted]
PHST- 2016/04/16 06:00 [entrez]
PHST- 2016/04/16 06:00 [pubmed]
PHST- 2017/03/01 06:00 [medline]
AID - 10.3892/ijmm.2016.2564 [doi]
PST - ppublish
SO  - Int J Mol Med. 2016 Jun;37(6):1669-76. doi: 10.3892/ijmm.2016.2564. Epub 2016 Apr 
      14.