PMID- 2708292
OWN - NLM
STAT- MEDLINE
DCOM- 19890526
LR  - 20161123
IS  - 0004-5756 (Print)
IS  - 0004-5756 (Linking)
VI  - 72
IP  - 2
DP  - 1989 Mar-Apr
TI  - Determination of 2,3,7,8-tetrachlorodibenzo-p-dioxin in fish, using electron 
      capture gas chromatography with confirmation by mass spectrometry: 
      interlaboratory study.
PG  - 394-8
AB  - An interlaboratory study of the determination of 
      2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in fish was conducted by 6 
      analysts in 4 laboratories using high resolution gas chromatography with electron 
      capture detection (HRGC-EC) for quantitative screening analysis. Samples 
      consisted of 3 Great Lakes channel catfish homogenates containing different 
      levels of bioincurred 2,3,7,8-TCDD; 1 of these was prepared in duplicate and 
      another was prepared both with and without standard 2,3,7,8-TCDD fortification 
      for a total of 5 samples per set. All methods used included addition of 
      1,3,7,8-TCDD surrogate (to correct for procedural losses) followed by ethanolic 
      KOH digestion and hexane extraction. Certain cleanup steps used, including 
      sulfuric acid washing and multidimensional column liquid chromatographic 
      procedures, varied among laboratories. Mean HRGC-EC results for the bioincurred 
      residues were 56.6, 25.2, and 7.7 pg/g (ppt) with corresponding relative standard 
      deviations (RSDs) of 9.1, 18.6, and 53.2%. Average determination of standard 
      2,3,7,8-TCDD from the fortified sample (corrected for surrogate recoveries 
      averaging 74.6%) was 106% of the added amount (30.9 pg/g) with 11.0% RSD. 
      HRGC-multiple ion detection mass spectrometry (MS), monitoring 12 ions, was used 
      for confirmation. With the exception of several results from 1 analyst, HRGC-MS 
      and HRGC-EC quantitations were in good agreement. All but 1 result reported met 
      all of the MS identity criteria.
FAU - Fehringer, N V
AU  - Fehringer NV
AD  - Food and Drug Administration, Pesticides and Industrial Chemicals Research 
      Center, Detroit, MI 48207.
FAU - Walters, S M
AU  - Walters SM
FAU - Niemann, R A
AU  - Niemann RA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Assoc Off Anal Chem
JT  - Journal - Association of Official Analytical Chemists
JID - 7505559
RN  - 0 (Dioxins)
RN  - 0 (Polychlorinated Dibenzodioxins)
SB  - IM
MH  - Animals
MH  - Chromatography, Gas
MH  - Dioxins/*analysis
MH  - *Fishes
MH  - Mass Spectrometry
MH  - Polychlorinated Dibenzodioxins/*analysis
EDAT- 1989/03/01 00:00
MHDA- 1989/03/01 00:01
CRDT- 1989/03/01 00:00
PHST- 1989/03/01 00:00 [pubmed]
PHST- 1989/03/01 00:01 [medline]
PHST- 1989/03/01 00:00 [entrez]
PST - ppublish
SO  - J Assoc Off Anal Chem. 1989 Mar-Apr;72(2):394-8.