PMID- 27087084
OWN - NLM
STAT- MEDLINE
DCOM- 20160630
LR  - 20160418
IS  - 1011-601X (Print)
IS  - 1011-601X (Linking)
VI  - 29
IP  - 2
DP  - 2016 Mar
TI  - Gas chromatography coupled with mass spectrometric characterization of Curcuma 
      longa: Protection against pathogenic microbes and lipid peroxidation in rat's 
      tissue homogenate.
PG  - 615-21
AB  - The present study was designed to investigate the mineral content and 
      antimicrobial activity of Curcuma Longa extracts and its essential oil. We also 
      determined the lipid peroxidation inhibition activity of the ethanolic extract 
      against sodium nitroprusside (SNP) induced thiobarbituric acid reactive species 
      (TBARS) formation in rat's brain, kidney and liver homogenates. Major 
      constituents of essential oil identified by gas chromatography and mass 
      spectrometry (GCMS) were beta-sesquiphellandrene (38.69%), alpha-curcumene 
      (18.44%) and p-mentha-1,4 (8)-diene (16.29%). Atomic absorption spectroscopy 
      (AAS) was used for the quantitative estimation of Calcium (Ca), Magnesium (Mg), 
      Iron (Fe), Copper (Cu), Zinc (Zn), Chromium (Cr), Nickel (Ni) and Manganese (Mn). 
      The extract showed highest Mg (49.4 mg/l) concentration followed by Ca (35.42 
      mg/l) and Fe (1.27 mg/l). Our data revealed that the ethanolic extract of Curcuma 
      Longa at 1-10 mg/kg significantly inhibited TBARS production in all tested 
      homogenates. Crude extracts and essential oil were tested against three gram 
      positive bacteria i.e. Bacillus subtilis, Bacillus atrophoeus, Staphylococcus 
      aureus, six gram negative bacteria i.e. Escherichia coli, Klebsiella pneumonias, 
      Salmonella typhi, Pseudomonas aeruginosa, Erwinia carotovora, Agrobacterium 
      tumefaciens and one fungal strain namely Candida albicans by disc diffusion 
      assay. Essential oil showed highest anti-microbial activity as compared to the 
      crude extracts. The present study confirms the significant antimicrobial and 
      antioxidant potential of the studied plant, which can be considered as a diet 
      supplement for a variety of oxidative stress induced or infectious diseases.
FAU - Hassan, Waseem
AU  - Hassan W
AD  - Institute of Chemical Sciences, University of Peshawar, Peshawar, Khyber 
      Pakhtunkhwa, Pakistan / Departamento de Quimica, Centro de Ciencias Naturais e 
      Exatas, Universidade Federal de Santa Maria, Santa Maria, CEP, RS, Brazil.
FAU - Gul, Shehnaz
AU  - Gul S
AD  - Institute of Chemical Sciences, University of Peshawar, Peshawar, Khyber 
      Pakhtunkhwa, Pakistan.
FAU - Rehman, Shakilla
AU  - Rehman S
AD  - Institute of Chemical Sciences, University of Peshawar, Peshawar, Khyber 
      Pakhtunkhwa, Pakistan.
FAU - Kanwal, Farina
AU  - Kanwal F
AD  - Food Technology Center, PCSIR Labs Complex, Jamrud Road, Peshawar, Pakistan.
FAU - Afridi, Muhammad Siddique
AU  - Afridi MS
AD  - Food Technology Center, PCSIR Labs Complex, Jamrud Road, Peshawar, Pakistan.
FAU - Fazal, Hina
AU  - Fazal H
AD  - Food Technology Center, PCSIR Labs Complex, Jamrud Road, Peshawar, Pakistan.
FAU - Shah, Ziarat
AU  - Shah Z
AD  - Institute of Chemical Sciences, University of Peshawar, Peshawar, Khyber 
      Pakhtunkhwa, Pakistan.
FAU - Rahman, Ataur
AU  - Rahman A
AD  - Institute of Chemical Sciences, University of Peshawar, Peshawar, Khyber 
      Pakhtunkhwa, Pakistan.
FAU - da Rocha, Joao B T
AU  - da Rocha JB
AD  - Food Technology Center, PCSIR Labs Complex, Jamrud Road, Peshawar, Pakistan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Pakistan
TA  - Pak J Pharm Sci
JT  - Pakistan journal of pharmaceutical sciences
JID - 9426356
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Oils, Volatile)
RN  - 0 (Plant Extracts)
RN  - 0 (Plant Oils)
RN  - 0 (Solvents)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 169D1260KM (Nitroprusside)
RN  - 3K9958V90M (Ethanol)
RN  - 4Y8F71G49Q (Malondialdehyde)
SB  - IM
MH  - Anti-Infective Agents/isolation & purification/*pharmacology
MH  - Antioxidants/isolation & purification/*pharmacology
MH  - Bacteria/drug effects/growth & development
MH  - Brain/drug effects/metabolism
MH  - Candida albicans/drug effects/growth & development
MH  - Curcuma/*chemistry
MH  - Disk Diffusion Antimicrobial Tests
MH  - Dose-Response Relationship, Drug
MH  - Ethanol/chemistry
MH  - *Gas Chromatography-Mass Spectrometry
MH  - Kidney/drug effects/metabolism
MH  - Lipid Peroxidation/*drug effects
MH  - Liver/drug effects/metabolism
MH  - Malondialdehyde/metabolism
MH  - Nitroprusside/pharmacology
MH  - Oils, Volatile/isolation & purification/*pharmacology
MH  - Oxidative Stress/*drug effects
MH  - Phytotherapy
MH  - Plant Extracts/isolation & purification/*pharmacology
MH  - Plant Oils/isolation & purification/*pharmacology
MH  - Plants, Medicinal
MH  - Solvents/chemistry
MH  - Spectrophotometry, Atomic
MH  - Thiobarbituric Acid Reactive Substances/metabolism
EDAT- 2016/04/19 06:00
MHDA- 2016/07/01 06:00
CRDT- 2016/04/19 06:00
PHST- 2016/04/19 06:00 [entrez]
PHST- 2016/04/19 06:00 [pubmed]
PHST- 2016/07/01 06:00 [medline]
PST - ppublish
SO  - Pak J Pharm Sci. 2016 Mar;29(2):615-21.