PMID- 27088093
OWN - NLM
STAT- MEDLINE
DCOM- 20161221
LR  - 20181202
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2016
DP  - 2016
TI  - Effect of Microenvironment on Differentiation of Human Umbilical Cord Mesenchymal 
      Stem Cells into Hepatocytes In Vitro and In Vivo.
PG  - 8916534
LID - 10.1155/2016/8916534 [doi]
LID - 8916534
AB  - Human umbilical cord-derived mesenchymal stem cells (hUCMSCs) are considered to 
      be an ideal cell source for cell therapy of many diseases. The aim of this study 
      was to investigate the contribution of the microenvironment to the hepatic 
      differentiation potential of hUCMSCs in vitro and in vivo and to explore their 
      therapeutic use in acute liver injury in rats. We established a new model to 
      simulate the liver tissue microenvironment in vivo using liver homogenate 
      supernatant (LHS) in vitro. This induced environment could drive hUCMSCs to 
      differentiate into hepatocyte-like cells within 7 days. The differentiated cells 
      expressed hepatocyte-specific markers and demonstrated hepatocellular functions. 
      We also injected hUCMSCs into rats with CCl4-induced acute hepatic injury. The 
      hUCMSCs were detected in the livers of recipient rats and expressed the human 
      hepatocyte-specific markers, suggesting that hUCMSCs could differentiate into 
      hepatocyte-like cells in vivo in the liver tissue microenvironment. Levels of 
      biochemistry markers improved significantly after transplantation of hUCMSCs 
      compared with the nontransplantation group (P < 0.05). In conclusion, this study 
      demonstrated that the liver tissue microenvironment may contribute to the 
      differentiation of hUCMSCs into hepatocytes both in vitro and in vivo.
FAU - Xue, Gai
AU  - Xue G
AD  - Graduate School, Hebei Medical University, Shijiazhuang, Hebei 050017, China; 
      Department of Pharmacy, Bethune International Peace Hospital, Shijiazhuang, Hebei 
      050082, China.
FAU - Han, Xiaolei
AU  - Han X
AD  - Department of Pharmacy, Bethune International Peace Hospital, Shijiazhuang, Hebei 
      050082, China.
FAU - Ma, Xin
AU  - Ma X
AD  - Department of Pharmacy, Bethune International Peace Hospital, Shijiazhuang, Hebei 
      050082, China.
FAU - Wu, Honghai
AU  - Wu H
AD  - Department of Pharmacy, Bethune International Peace Hospital, Shijiazhuang, Hebei 
      050082, China.
FAU - Qin, Yabin
AU  - Qin Y
AD  - Department of Pharmacy, Bethune International Peace Hospital, Shijiazhuang, Hebei 
      050082, China.
FAU - Liu, Jianfang
AU  - Liu J
AD  - Department of Pharmacy, Bethune International Peace Hospital, Shijiazhuang, Hebei 
      050082, China.
FAU - Hu, Yuqin
AU  - Hu Y
AD  - Department of Pharmacy, Bethune International Peace Hospital, Shijiazhuang, Hebei 
      050082, China.
FAU - Hong, Yang
AU  - Hong Y
AD  - Graduate School, Hebei Medical University, Shijiazhuang, Hebei 050017, China.
FAU - Hou, Yanning
AU  - Hou Y
AD  - Graduate School, Hebei Medical University, Shijiazhuang, Hebei 050017, China; 
      Department of Pharmacy, Bethune International Peace Hospital, Shijiazhuang, Hebei 
      050082, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160210
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Biomarkers)
RN  - CL2T97X0V0 (Carbon Tetrachloride)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Carbon Tetrachloride/toxicity
MH  - Cell Differentiation/genetics
MH  - Chemical and Drug Induced Liver Injury/metabolism/*therapy
MH  - *Cord Blood Stem Cell Transplantation
MH  - Hepatocytes/cytology
MH  - Humans
MH  - Liver/pathology
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Rats
MH  - Umbilical Cord/*cytology
PMC - PMC4764721
EDAT- 2016/04/19 06:00
MHDA- 2016/12/22 06:00
PMCR- 2016/02/10
CRDT- 2016/04/19 06:00
PHST- 2015/08/09 00:00 [received]
PHST- 2015/12/21 00:00 [revised]
PHST- 2015/12/29 00:00 [accepted]
PHST- 2016/04/19 06:00 [entrez]
PHST- 2016/04/19 06:00 [pubmed]
PHST- 2016/12/22 06:00 [medline]
PHST- 2016/02/10 00:00 [pmc-release]
AID - 10.1155/2016/8916534 [doi]
PST - ppublish
SO  - Biomed Res Int. 2016;2016:8916534. doi: 10.1155/2016/8916534. Epub 2016 Feb 10.