PMID- 27090759
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181202
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 92
IP  - 6
DP  - 2016 Jun
TI  - Phylogenetic diversity and ecophysiology of Candidate phylum Saccharibacteria in 
      activated sludge.
PG  - fiw078
LID - fiw078 [pii]
LID - 10.1093/femsec/fiw078 [doi]
AB  - Candidate phylum Saccharibacteria (former TM7) are abundant and widespread in 
      nature, but little is known about their ecophysiology and detailed phylogeny. In 
      this study phylogeny, morphology and ecophysiology of Saccharibacteria were 
      investigated in activated sludge from nine wastewater treatment plants (WWTPs) 
      from Japan and Denmark using the full-cycle 16S rRNA approach in combination with 
      microautoradiography (MAR) and fluorescence in situ hybridization (FISH). 
      Phylogenetic analysis showed that Saccharibacteria from all WWTPs were evenly 
      distributed within subdivision 1 and 3 and in a distinct phylogenetic clade. 
      Three probes were designed for the distinct saccharibacterial groups, and 
      revealed morphotypes representing thin filaments, thick filaments and rods/cocci. 
      MAR-FISH results showed that most probe-defined Saccharibacteria utilized glucose 
      under aerobic-, nitrate reducing- and anaerobic conditions. Some Saccharibacteria 
      also utilized N-acetylglucosamine, oleic acid, amino acids and butyrate, which 
      are not predicted from available genomes so far. In addition, some filamentous 
      Saccharibacteria exhibited beta-galactosidase and lipase activities determined using 
      a combination of enzyme-labeled fluorescence and FISH (ELF-FISH). No uptake of 
      acetate, propionate, pyruvate, glycerol and ethanol was observed. These results 
      indicate that Saccharibacteria is a phylogenetically diverse group and play a 
      role in the degradation of various organic compounds as well as sugar compounds 
      under aerobic-, nitrate reducing- and anaerobic conditions.
CI  - (c) FEMS 2016. All rights reserved. For permissions, please e-mail: 
      journals.permissions@oup.com.
FAU - Kindaichi, Tomonori
AU  - Kindaichi T
AD  - Department of Civil and Environmental Engineering, Hiroshima University, 1-4-1 
      Kagamiyama, Higashihiroshima, 739-8527 Japan Center for Microbial Communities, 
      Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 
      7H, DK-9220 Aalborg E, Denmark.
FAU - Yamaoka, Shiro
AU  - Yamaoka S
AD  - Department of Civil and Environmental Engineering, Hiroshima University, 1-4-1 
      Kagamiyama, Higashihiroshima, 739-8527 Japan.
FAU - Uehara, Ryohei
AU  - Uehara R
AD  - Department of Civil and Environmental Engineering, Hiroshima University, 1-4-1 
      Kagamiyama, Higashihiroshima, 739-8527 Japan.
FAU - Ozaki, Noriatsu
AU  - Ozaki N
AD  - Department of Civil and Environmental Engineering, Hiroshima University, 1-4-1 
      Kagamiyama, Higashihiroshima, 739-8527 Japan.
FAU - Ohashi, Akiyoshi
AU  - Ohashi A
AD  - Department of Civil and Environmental Engineering, Hiroshima University, 1-4-1 
      Kagamiyama, Higashihiroshima, 739-8527 Japan.
FAU - Albertsen, Mads
AU  - Albertsen M
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University, Fredrik Bajers Vej 7H, DK-9220 Aalborg E, Denmark.
FAU - Nielsen, Per Halkjaer
AU  - Nielsen PH
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University, Fredrik Bajers Vej 7H, DK-9220 Aalborg E, Denmark.
FAU - Nielsen, Jeppe Lund
AU  - Nielsen JL
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University, Fredrik Bajers Vej 7H, DK-9220 Aalborg E, Denmark jln@bio.aau.dk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160417
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - EC 3.1.1.3 (Lipase)
RN  - EC 3.2.1.- (Galactosidases)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Autoradiography
MH  - *Bacteria/classification/genetics/metabolism
MH  - DNA, Bacterial/genetics
MH  - Denmark
MH  - Energy Metabolism/*genetics
MH  - Galactosidases/metabolism
MH  - Glucose/metabolism
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Japan
MH  - Lipase/metabolism
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/*microbiology
OTO - NOTNLM
OT  - FISH
OT  - Saccharibacteria
OT  - activated sludge
OT  - candidate division TM7
OT  - exoenzyme activity
OT  - microautoradiography
EDAT- 2016/04/20 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/04/20 06:00
PHST- 2016/04/14 00:00 [accepted]
PHST- 2016/04/20 06:00 [entrez]
PHST- 2016/04/20 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - fiw078 [pii]
AID - 10.1093/femsec/fiw078 [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2016 Jun;92(6):fiw078. doi: 10.1093/femsec/fiw078. Epub 2016 
      Apr 17.