PMID- 27092486
OWN - NLM
STAT- MEDLINE
DCOM- 20170106
LR  - 20181113
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 17
IP  - 4
DP  - 2016 Apr 15
TI  - Profilings of MicroRNAs in the Liver of Common Carp (Cyprinus carpio) Infected 
      with Flavobacterium columnare.
PG  - 566
LID - 10.3390/ijms17040566 [doi]
LID - 566
AB  - MicroRNAs (miRNAs) play important roles in regulation of many biological 
      processes in eukaryotes, including pathogen infection and host interactions. 
      Flavobacterium columnare (FC) infection can cause great economic loss of common 
      carp (Cyprinus carpio) which is one of the most important cultured fish in the 
      world. However, miRNAs in response to FC infection in common carp has not been 
      characterized. To identify specific miRNAs involved in common carp infected with 
      FC, we performed microRNA sequencing using livers of common carp infected with 
      and without FC. A total of 698 miRNAs were identified, including 142 which were 
      identified and deposited in the miRbase database (Available online: 
      http://www.mirbase.org/) and 556 had only predicted miRNAs. Among the deposited 
      miRNAs, eight miRNAs were first identified in common carp. Thirty of the 698 
      miRNAs were differentially expressed miRNAs (DIE-miRNAs) between the FC infected 
      and control samples. From the DIE-miRNAs, seven were selected randomly and their 
      expression profiles were confirmed to be consistent with the microRNA sequencing 
      results using RT-PCR and qRT-PCR. In addition, a total of 27,363 target genes of 
      the 30 DIE-miRNAs were predicted. The target genes were enriched in five Kyoto 
      Encyclopedia of Genes and Genomes (KEGG) pathways, including focal adhesion, 
      extracellular matrix (ECM)-receptor interaction, erythroblastic leukemia viral 
      oncogene homolog (ErbB) signaling pathway, regulation of actin cytoskeleton, and 
      adherent junction. The miRNA expression profile of the liver of common carp 
      infected with FC will pave the way for the development of effective strategies to 
      fight against FC infection.
FAU - Zhao, Lijuan
AU  - Zhao L
AD  - Shandong Freshwater Fisheries Research Institute, Jinan 250013, China. 
      zhaolijuan4234@163.com.
AD  - Shandong Provincial Key Laboratory of Freshwater Genetics and Breeding, Jinan 
      250013, China. zhaolijuan4234@163.com.
AD  - Shandong Provincial Freshwater Aquatic Products Quality Inspection Center, Jinan 
      250013, China. zhaolijuan4234@163.com.
AD  - Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong 
      Agricultural University, Wuhan 430070, China. zhaolijuan4234@163.com.
FAU - Lu, Hong
AU  - Lu H
AD  - Shandong Freshwater Fisheries Research Institute, Jinan 250013, China. 
      luhong.518@163.com.
AD  - Shandong Provincial Key Laboratory of Freshwater Genetics and Breeding, Jinan 
      250013, China. luhong.518@163.com.
AD  - Shandong Provincial Freshwater Aquatic Products Quality Inspection Center, Jinan 
      250013, China. luhong.518@163.com.
FAU - Meng, Qinglei
AU  - Meng Q
AD  - Shandong Freshwater Fisheries Research Institute, Jinan 250013, China. 
      qingleimeng@126.com.
AD  - Shandong Provincial Key Laboratory of Freshwater Genetics and Breeding, Jinan 
      250013, China. qingleimeng@126.com.
AD  - Shandong Provincial Freshwater Aquatic Products Quality Inspection Center, Jinan 
      250013, China. qingleimeng@126.com.
FAU - Wang, Jinfu
AU  - Wang J
AD  - Shandong Freshwater Fisheries Research Institute, Jinan 250013, China. 
      wangjinfu1978@163.com.
AD  - Shandong Provincial Key Laboratory of Freshwater Genetics and Breeding, Jinan 
      250013, China. wangjinfu1978@163.com.
AD  - Shandong Provincial Freshwater Aquatic Products Quality Inspection Center, Jinan 
      250013, China. wangjinfu1978@163.com.
FAU - Wang, Weimin
AU  - Wang W
AD  - Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong 
      Agricultural University, Wuhan 430070, China. wangwm@mail.hzau.edu.cn.
FAU - Yang, Ling
AU  - Yang L
AD  - Shandong Freshwater Fisheries Research Institute, Jinan 250013, China. 
      jnsyang@163.com.
AD  - Shandong Provincial Key Laboratory of Freshwater Genetics and Breeding, Jinan 
      250013, China. jnsyang@163.com.
AD  - Shandong Provincial Freshwater Aquatic Products Quality Inspection Center, Jinan 
      250013, China. jnsyang@163.com.
FAU - Lin, Li
AU  - Lin L
AD  - Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong 
      Agricultural University, Wuhan 430070, China. linli@mail.hzau.edu.cn.
AD  - Department of Aquatic Animal Medicine, College of Fisheries, Huazhong 
      Agricultural University, Wuhan 430070, China. linli@mail.hzau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160415
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Carps/*genetics/*microbiology
MH  - Fish Diseases/*genetics/microbiology
MH  - Flavobacteriaceae Infections/genetics/*veterinary
MH  - Flavobacterium/*physiology
MH  - Gene Expression Profiling
MH  - Liver/metabolism/microbiology
MH  - MicroRNAs/*genetics
PMC - PMC4849022
OTO - NOTNLM
OT  - Flavobacterium columnare
OT  - common carp
OT  - deep sequencing
OT  - liver
OT  - miRNA
EDAT- 2016/04/20 06:00
MHDA- 2017/01/07 06:00
PMCR- 2016/04/01
CRDT- 2016/04/20 06:00
PHST- 2016/01/14 00:00 [received]
PHST- 2016/03/20 00:00 [revised]
PHST- 2016/04/08 00:00 [accepted]
PHST- 2016/04/20 06:00 [entrez]
PHST- 2016/04/20 06:00 [pubmed]
PHST- 2017/01/07 06:00 [medline]
PHST- 2016/04/01 00:00 [pmc-release]
AID - ijms17040566 [pii]
AID - ijms-17-00566 [pii]
AID - 10.3390/ijms17040566 [doi]
PST - epublish
SO  - Int J Mol Sci. 2016 Apr 15;17(4):566. doi: 10.3390/ijms17040566.