PMID- 27096327 OWN - NLM STAT- MEDLINE DCOM- 20170213 LR - 20201209 IS - 1205-7541 (Electronic) IS - 0008-4212 (Print) IS - 0008-4212 (Linking) VI - 94 IP - 6 DP - 2016 Jun TI - The effect of matrix metalloproteinase-3 deficiency on pulmonary surfactant in a mouse model of acute lung injury. PG - 682-5 LID - 10.1139/cjpp-2015-0377 [doi] AB - The acute respiratory distress syndrome (ARDS) is characterized by arterial hypoxemia accompanied by severe inflammation and alterations to the pulmonary surfactant system. Published data has demonstrated a protective effect of matrix metalloproteinase-3 (Mmp3) deficiency against the inflammatory response associated with ARDS; however, the effect of Mmp3 on physiologic parameters and alterations to surfactant have not been previously studied. It was hypothesized that Mmp3 deficient (Mmp3(-/-)) mice would be protected against lung dysfunction associated with ARDS and maintain a functional pulmonary surfactant system. Wild type (WT) and Mmp3(-/-) mice were subjected to acid-aspiration followed by mechanical ventilation. Mmp3(-/-) mice maintained higher arterial oxygenation compared with WT mice at the completion of ventilation. Significant increase in functional large aggregate surfactant forms were observed in Mmp3(-/-) mice compared with WT mice. These findings further support a role of Mmp3 as an attractive therapeutic target for drug development in the setting of ARDS. FAU - Yamashita, Cory M AU - Yamashita CM AD - a Lawson Health Research Institute, Department of Physiology and Pharmacology, Western University, London ON, Canada. AD - c Department of Medicine, Western University, London ON, Canada. FAU - Cybulskie, Candice AU - Cybulskie C AD - a Lawson Health Research Institute, Department of Physiology and Pharmacology, Western University, London ON, Canada. FAU - Milos, Scott AU - Milos S AD - a Lawson Health Research Institute, Department of Physiology and Pharmacology, Western University, London ON, Canada. FAU - Zuo, Yi Y AU - Zuo YY AD - b Department of Mechanical Engineering, University of Hawaii at Manoa, Honolulu, HI, USA. FAU - McCaig, Lynda A AU - McCaig LA AD - a Lawson Health Research Institute, Department of Physiology and Pharmacology, Western University, London ON, Canada. FAU - Veldhuizen, Ruud A W AU - Veldhuizen RA AD - a Lawson Health Research Institute, Department of Physiology and Pharmacology, Western University, London ON, Canada. AD - c Department of Medicine, Western University, London ON, Canada. LA - eng GR - U54 MD007584/MD/NIMHD NIH HHS/United States PT - Journal Article DEP - 20160119 PL - Canada TA - Can J Physiol Pharmacol JT - Canadian journal of physiology and pharmacology JID - 0372712 RN - 0 (Pulmonary Surfactants) RN - EC 3.4.24.17 (Matrix Metalloproteinase 3) RN - EC 3.4.24.17 (Mmp3 protein, mouse) SB - IM MH - Acute Lung Injury/*metabolism/pathology MH - Animals MH - Bronchoalveolar Lavage Fluid MH - *Disease Models, Animal MH - Female MH - Matrix Metalloproteinase 3/*deficiency MH - Mice MH - Mice, Inbred C57BL MH - Mice, Knockout MH - Pulmonary Surfactants/*metabolism MH - Random Allocation MH - Respiratory Distress Syndrome/*metabolism/pathology PMC - PMC5522962 MID - NIHMS871297 OTO - NOTNLM OT - acid aspiration OT - acute lung injury OT - acute respiratory distress syndrome OT - aspiration d'acide OT - hypoxemia OT - hypoxemie OT - lesion pulmonaire aigue OT - matrix metalloproteinase-3 OT - mechanical ventilation OT - metalloproteinase matricielle-3 OT - pulmonary surfactant OT - surfactant pulmonaire OT - syndrome de detresse respiratoire aigue OT - ventilation mecanique EDAT- 2016/04/21 06:00 MHDA- 2017/02/14 06:00 PMCR- 2017/07/24 CRDT- 2016/04/21 06:00 PHST- 2016/04/21 06:00 [entrez] PHST- 2016/04/21 06:00 [pubmed] PHST- 2017/02/14 06:00 [medline] PHST- 2017/07/24 00:00 [pmc-release] AID - 10.1139/cjpp-2015-0377 [doi] PST - ppublish SO - Can J Physiol Pharmacol. 2016 Jun;94(6):682-5. doi: 10.1139/cjpp-2015-0377. Epub 2016 Jan 19.