PMID- 27096955
OWN - NLM
STAT- MEDLINE
DCOM- 20171226
LR  - 20211204
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 7
IP  - 22
DP  - 2016 May 31
TI  - Fluid shear stress induces epithelial-mesenchymal transition (EMT) in Hep-2 
      cells.
PG  - 32876-92
LID - 10.18632/oncotarget.8765 [doi]
AB  - Laryngeal squamous cell carcinoma (LSCC) is one of the most commonly diagnosed 
      malignancies with high occurrence of tumor metastasis, which usually exposes to 
      fluid shear stress (FSS) in lymphatic channel and blood vessel. 
      Epithelial-mesenchymal transition (EMT) is an important mechanism that induces 
      metastasis and invasion of tumors. We hypothesized that FSS induced a progression 
      of EMT in laryngeal squamous carcinoma. Accordingly, the Hep-2 cells were exposed 
      to 1.4 dyn/cm2 FSS for different durations. Our results showed that most of cells 
      changed their morphology from polygon to elongated spindle with well-organized 
      F-actin and abundant lamellipodia/filopodia in protrusions. After removing the 
      FSS, cells gradually recovered their flat polygon morphology. FSS induced Hep-2 
      cells to enhance their migration capacity in a time-dependent manner. In 
      addition, FSS down-regulated E-cadherin, and simultaneously up-regulated 
      N-cadherin, translocated beta-catenin into the nucleus. These results confirmed that 
      FSS induced the EMT in Hep-2 cells, and revealed a reversible 
      mesenchymal-epithelial transition (MET) process when FSS was removed. We further 
      examined the time-expressions of signaling cascades, and demonstrated that FSS 
      induces the EMT and enhances cell migration depending on 
      integrin-ILK/PI3K-AKT-Snail signaling events. The current study suggests that 
      FSS, an important biophysical factor in tumor microenvironment, is a potential 
      determinant of cell behavior and function regulation.
FAU - Liu, Shuangfeng
AU  - Liu S
AD  - Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, 
      Sichuan University, Chengdu 610041, China.
AD  - School of Medical Laboratory Science, Chengdu Medical College, Chengdu 610500, 
      China.
FAU - Zhou, Fating
AU  - Zhou F
AD  - Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, 
      Sichuan University, Chengdu 610041, China.
FAU - Shen, Yang
AU  - Shen Y
AD  - Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, 
      Sichuan University, Chengdu 610041, China.
FAU - Zhang, Yingying
AU  - Zhang Y
AD  - Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, 
      Sichuan University, Chengdu 610041, China.
FAU - Yin, Hongmei
AU  - Yin H
AD  - West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
FAU - Zeng, Ye
AU  - Zeng Y
AD  - Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, 
      Sichuan University, Chengdu 610041, China.
FAU - Liu, Jingxia
AU  - Liu J
AD  - Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, 
      Sichuan University, Chengdu 610041, China.
FAU - Yan, Zhiping
AU  - Yan Z
AD  - Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, 
      Sichuan University, Chengdu 610041, China.
FAU - Liu, Xiaoheng
AU  - Liu X
AD  - Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, 
      Sichuan University, Chengdu 610041, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
RN  - 0 (Antigens, CD)
RN  - 0 (CDH1 protein, human)
RN  - 0 (CDH2 protein, human)
RN  - 0 (CTNNB1 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (Snail Family Transcription Factors)
RN  - 0 (beta Catenin)
RN  - EC 2.7.1.- (integrin-linked kinase)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Antigens, CD/metabolism
MH  - Cadherins/metabolism
MH  - Carcinoma, Squamous Cell/genetics/metabolism/*pathology
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Shape
MH  - *Epithelial-Mesenchymal Transition
MH  - Head and Neck Neoplasms/genetics/metabolism/*pathology
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/metabolism/*pathology
MH  - *Mechanotransduction, Cellular
MH  - Phosphatidylinositol 3-Kinase/metabolism
MH  - Protein Serine-Threonine Kinases/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Pseudopodia/metabolism/pathology
MH  - RNA Interference
MH  - Snail Family Transcription Factors/genetics/metabolism
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Stress, Mechanical
MH  - Time Factors
MH  - Transfection
MH  - Tumor Microenvironment
MH  - beta Catenin/metabolism
PMC - PMC5078059
OTO - NOTNLM
OT  - cell migration
OT  - epithelial-mesenchymal transition (EMT)
OT  - fluid shear stress (FSS)
OT  - laryngeal squamous cell carcinomas (LSCC)
COIS- The authors declare that have no conflicts of interest.
EDAT- 2016/04/21 06:00
MHDA- 2017/12/27 06:00
PMCR- 2016/05/31
CRDT- 2016/04/21 06:00
PHST- 2015/07/21 00:00 [received]
PHST- 2016/03/28 00:00 [accepted]
PHST- 2016/04/21 06:00 [entrez]
PHST- 2016/04/21 06:00 [pubmed]
PHST- 2017/12/27 06:00 [medline]
PHST- 2016/05/31 00:00 [pmc-release]
AID - 8765 [pii]
AID - 10.18632/oncotarget.8765 [doi]
PST - ppublish
SO  - Oncotarget. 2016 May 31;7(22):32876-92. doi: 10.18632/oncotarget.8765.