PMID- 27097373
OWN - NLM
STAT- MEDLINE
DCOM- 20170106
LR  - 20200930
IS  - 1551-4005 (Electronic)
IS  - 1538-4101 (Print)
IS  - 1551-4005 (Linking)
VI  - 15
IP  - 8
DP  - 2016
TI  - Structure of the human dimeric ATM kinase.
PG  - 1117-24
LID - 10.1080/15384101.2016.1158362 [doi]
AB  - DNA-double strand breaks activate the serine/threonine protein kinase 
      ataxia-telangiectasia mutated (ATM) to initiate DNA damage signal transduction. 
      This activation process involves autophosphorylation and dissociation of inert 
      ATM dimers into monomers that are catalytically active. Using single-particle 
      electron microscopy (EM), we determined the structure of dimeric ATM in its 
      resting state. The EM map could accommodate the crystal structure of the 
      N-terminal truncated mammalian target of rapamycin (mTOR), a closely related 
      enzyme of the phosphatidylinositol 3-kinase-related protein kinase (PIKK) family, 
      allowing for the localization of the N- and the C-terminal regions of ATM. In the 
      dimeric structure, the actives sites are buried, restricting the access of the 
      substrates to these sites. The unanticipated domain organization of ATM provides 
      a basis for understanding its mechanism of inhibition.
FAU - Lau, Wilson C Y
AU  - Lau WC
AD  - a School of Biomedical Sciences, The University of Hong Kong , Hong Kong.
AD  - b State Key Laboratory of Brain and Cognitive Sciences, The University of Hong 
      Kong , Hong Kong.
FAU - Li, Yinyin
AU  - Li Y
AD  - c State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University , Guangzhou , China.
FAU - Liu, Zhe
AU  - Liu Z
AD  - d Guangdong Provincial Center for Disease Control and Prevention , Guangzhou , 
      China.
FAU - Gao, Yuanzhu
AU  - Gao Y
AD  - c State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University , Guangzhou , China.
FAU - Zhang, Qinfen
AU  - Zhang Q
AD  - c State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University , Guangzhou , China.
FAU - Huen, Michael S Y
AU  - Huen MS
AD  - a School of Biomedical Sciences, The University of Hong Kong , Hong Kong.
AD  - b State Key Laboratory of Brain and Cognitive Sciences, The University of Hong 
      Kong , Hong Kong.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Cycle
JT  - Cell cycle (Georgetown, Tex.)
JID - 101137841
RN  - 0 (Protein Subunits)
RN  - EC 2.7.11.1 (Ataxia Telangiectasia Mutated Proteins)
SB  - IM
MH  - Ataxia Telangiectasia Mutated Proteins/*chemistry/metabolism/ultrastructure
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Models, Molecular
MH  - Oxidation-Reduction
MH  - *Protein Multimerization
MH  - Protein Subunits/metabolism
PMC - PMC4889239
OTO - NOTNLM
OT  - ATM kinase
OT  - DNA damage
OT  - DNA damage response
OT  - PIKK
OT  - ataxia telangiectasia
OT  - electron microscopy (EM)
OT  - post-translational modification (PTM)
EDAT- 2016/04/21 06:00
MHDA- 2017/01/07 06:00
PMCR- 2016/04/20
CRDT- 2016/04/21 06:00
PHST- 2016/04/21 06:00 [entrez]
PHST- 2016/04/21 06:00 [pubmed]
PHST- 2017/01/07 06:00 [medline]
PHST- 2016/04/20 00:00 [pmc-release]
AID - 1158362 [pii]
AID - 10.1080/15384101.2016.1158362 [doi]
PST - ppublish
SO  - Cell Cycle. 2016;15(8):1117-24. doi: 10.1080/15384101.2016.1158362.