PMID- 27097457
OWN - NLM
STAT- MEDLINE
DCOM- 20160504
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 33
IP  - 12
DP  - 2015 Dec
TI  - [Simultaneous determination of 121 veterinary drugs in pork by QuEChERS and ultra 
      performance liquid chromatography-tandem mass spectrometry].
PG  - 1242-50
AB  - A method has been developed for the simultaneous determination of the 10 kinds of 
      121 veterinary drugs (including chloramphenicols, beta-agonists, sulfonamides, 
      4-quinolones, nitroimidazoles, macrolides, avermections, polyether antibiotics, 
      tetracycline antibiotics, cephalosporins, etc.) in pork using QuEChERS method 
      coupled with ultra performance liquid chromatography-tandem mass spectrometry 
      (UPLC-MS/MS). The drugs were extracted with Na2EDTA-McIlvaine buffer solution (pH 
      = 4) and acetonitrile. Anhydrous sodium sulfate was used for the salting-out 
      process. The solutions divided into two groups were cleaned-up by different mixed 
      sorbents. The drugs were analyzed by UPLC-MS/MS in multiple reaction monitoring 
      (MRM) mode via electrospray ionization. Among the 121 drugs, 5 drugs were 
      quantified by internal standard method, and the other 116 drugs were quantified 
      by external standard method. The calibration curves of the 121 drugs were linear 
      in the range of 0.02-40 mug/L with the correlation coefficients more than 0.99. 
      The limits of quantification (LOQs, S/N = 10) were 0.05-10 mug/kg. The average 
      recoveries of 8 drugs at LOQ level in pork ranged from 41.7% to 59.6% with the 
      relative standard deviations (RSDs) of 1.7% - 13.5%. The average recoveries of 10 
      drugs at LOQ level in pork ranged from 122.6% to 163.2% with the RSDs of 0.6% 
      -14.8%. The average recoveries of 103 drugs at LOQ level in pork ranged from 
      60.3% to 118.3% with the RSDs of 0.4% - 16.7%. The proposed method is rapid, 
      simple, sensitive, reliable and cost-effective.
FAU - Guo, Haixia
AU  - Guo H
FAU - Xiao, Guiying
AU  - Xiao G
FAU - Zhang, Xiqing
AU  - Zhang X
FAU - Wang, Minglin
AU  - Wang M
FAU - Li, Li
AU  - Li L
FAU - Leng, Lei
AU  - Leng L
FAU - Gao, Hongliang
AU  - Gao H
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Macrolides)
RN  - 0 (Sulfonamides)
RN  - 0 (Veterinary Drugs)
RN  - 66974FR9Q1 (Chloramphenicol)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents
MH  - Chloramphenicol
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid
MH  - Drug Residues/*analysis
MH  - Macrolides
MH  - Red Meat/*analysis
MH  - Sulfonamides
MH  - Swine
MH  - Tandem Mass Spectrometry
MH  - Veterinary Drugs/*analysis
EDAT- 2016/04/22 06:00
MHDA- 2016/05/05 06:00
CRDT- 2016/04/22 06:00
PHST- 2016/04/22 06:00 [entrez]
PHST- 2016/04/22 06:00 [pubmed]
PHST- 2016/05/05 06:00 [medline]
AID - 10.3724/sp.j.1123.2015.09016 [doi]
PST - ppublish
SO  - Se Pu. 2015 Dec;33(12):1242-50. doi: 10.3724/sp.j.1123.2015.09016.