PMID- 27097467
OWN - NLM
STAT- MEDLINE
DCOM- 20160504
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 33
IP  - 12
DP  - 2015 Dec
TI  - [Simultaneous determination of four aflatoxins in Citrus reticulatablanco by 
      ultra performance liquid chromatography coupled with immunomagnetic beads for 
      enrichment and purification].
PG  - 1320-6
AB  - Immunomagnetic beads (IMBs) were formed by coupling the ProtElut NHS 
      (N-hydroxysuccinimide) magnetic beads and anti-aflatoxins monoclonal antibody. 
      The synthesized IMBs presented uniform size, good magnetic property and specific 
      selectivity. In this work, a novel and facile pretreatment method of sample 
      enrichment and purification based on the IMBs for the determination of aflatoxins 
      (AFs) (AFB1, AFB2, AFG1, AFG2) in Citrus reticulatablanco samples by ultra 
      performance liquid chromatography (UPLC) were developed. The sample was extracted 
      with 20% (v/v) methanol-PBS buffer solution (pH 7.4), followed by a cleanup 
      procedure with the IMBs. The target compounds were eluted using 1 mL methanol. 
      The four afatoxins were separated on an ACQUITY UPLC HSS T3 C18 column (100 mm x 
      2.1 mm, 1.8 mum) adopting a gradient program within 12 min. The UPLC equipped with 
      a fluorescence detector with a special mercury/xenon lamp was used to detect the 
      aflatoxins. The satisfactory correlation coefficients (r2 > 0.999) of the four 
      aflatoxins were obtained within their respective linear ranges. The limits of 
      detection (S/N = 3) were between 0.013 mug/kg and 0.038 mug/kg, and the limits of 
      quantification (S/N = 10) were between 0.044 mug/kg and 1.2 mug/kg. The recoveries 
      were in the range of 63.9% - 115.0% with the relative standard deviations (RSDs) 
      of 0.4% - 14.2%. The validation results meet the requirements of trace assay. 
      It's indicated that the IMBs were good pretreatment alternatives and the 
      developed method is simple, fast, accurate, and can be applied for the 
      simultaneous determination of the four aflatoxins in Citrus reticulatablanco.
FAU - Xing, Yanyan
AU  - Xing Y
FAU - Tong, Ling
AU  - Tong L
FAU - Chen, Nan
AU  - Chen N
FAU - Yu, Zhiguo
AU  - Yu Z
FAU - Zhao, Yunli
AU  - Zhao Y
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Aflatoxins)
SB  - IM
MH  - Aflatoxins/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - Citrus/*chemistry
MH  - Immunomagnetic Separation
EDAT- 2016/04/22 06:00
MHDA- 2016/05/05 06:00
CRDT- 2016/04/22 06:00
PHST- 2016/04/22 06:00 [entrez]
PHST- 2016/04/22 06:00 [pubmed]
PHST- 2016/05/05 06:00 [medline]
AID - 10.3724/sp.j.1123.2015.06001 [doi]
PST - ppublish
SO  - Se Pu. 2015 Dec;33(12):1320-6. doi: 10.3724/sp.j.1123.2015.06001.