PMID- 27098433
OWN - NLM
STAT- MEDLINE
DCOM- 20170724
LR  - 20171205
IS  - 1742-2051 (Electronic)
IS  - 1742-2051 (Linking)
VI  - 12
IP  - 6
DP  - 2016 May 24
TI  - An autoregulatory network between menin and pri-miR-24-1 is required for the 
      processing of its specific modulator miR-24-1 in BON1 cells.
PG  - 1922-8
LID - 10.1039/c6mb00118a [doi]
AB  - Multiple endocrine neoplasia type 1 (MEN1) is a rare hereditary cancer complex 
      syndrome manifesting a variety of endocrine and non-endocrine neoplasms and 
      lesions. MEN1 is characterized by tumours of the parathyroids, of the 
      neuroendocrine cells of the gastroenteropancreatic tract, and of the anterior 
      pituitary. The MEN1 gene, a tumour suppressor gene, encodes the menin protein. 
      Loss of heterozygosity (LOH) at 11q13 is typical of MEN1 tumours in agreement 
      with Knudson's two-hit hypothesis. We previously showed that the MEN1 parathyroid 
      tumorigenesis is under the control of an "incoherent feedback loop" between 
      miR-24-1 and the menin protein that generates a "Gene Regulatory Network" (GRN) 
      that mimics the second hit of Knudson's hypothesis and that could buffer the 
      effect of the stochastic factors that contribute to the onset and progression of 
      this disease. Here we show, in the BON1 cell line derived from lymphnode 
      metastasis of a human carcinoid tumour of the pancreas, that menin binds 
      specifically to the primary RNA sequence pri-miR-24-1 by promoting the miR-24-1 
      biogenesis. Network simulation showed a new feed-forward loop between menin, 
      microRNA-24-1 and Musashi-1 proteins. This result shows a novel mechanism whereby 
      menin, a RNA-binding protein, facilitates the processing of its specific miRNA by 
      regulating the dynamics of the menin-miR-24 Gene Regulatory Network at the level 
      of pri-miRNA processing.
FAU - Luzi, Ettore
AU  - Luzi E
AD  - Department of Surgery and Translational Medicine, University of Florence, Largo 
      Brambilla 3, 50134 Florence, Italy. eluzi@unifi.it francesca.marini@dmsc.unifi.it 
      simone.ciuffi@unifi.it gianna.galli@unifi.it marialuisa.brandi@unifi.it.
FAU - Marini, Francesca
AU  - Marini F
AD  - Department of Surgery and Translational Medicine, University of Florence, Largo 
      Brambilla 3, 50134 Florence, Italy. eluzi@unifi.it francesca.marini@dmsc.unifi.it 
      simone.ciuffi@unifi.it gianna.galli@unifi.it marialuisa.brandi@unifi.it.
FAU - Ciuffi, Simone
AU  - Ciuffi S
AD  - Department of Surgery and Translational Medicine, University of Florence, Largo 
      Brambilla 3, 50134 Florence, Italy. eluzi@unifi.it francesca.marini@dmsc.unifi.it 
      simone.ciuffi@unifi.it gianna.galli@unifi.it marialuisa.brandi@unifi.it.
FAU - Galli, Gianna
AU  - Galli G
AD  - Department of Surgery and Translational Medicine, University of Florence, Largo 
      Brambilla 3, 50134 Florence, Italy. eluzi@unifi.it francesca.marini@dmsc.unifi.it 
      simone.ciuffi@unifi.it gianna.galli@unifi.it marialuisa.brandi@unifi.it.
FAU - Brandi, Maria Luisa
AU  - Brandi ML
AD  - Department of Surgery and Translational Medicine, University of Florence, Largo 
      Brambilla 3, 50134 Florence, Italy. eluzi@unifi.it francesca.marini@dmsc.unifi.it 
      simone.ciuffi@unifi.it gianna.galli@unifi.it marialuisa.brandi@unifi.it.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Mol Biosyst
JT  - Molecular bioSystems
JID - 101251620
RN  - 0 (MEN1 protein, human)
RN  - 0 (MIRN24 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Cell Line, Tumor
MH  - Chromosomes, Human, Pair 11
MH  - *Gene Expression Regulation
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Microsatellite Repeats
MH  - Mutation
MH  - Protein Binding
MH  - Proto-Oncogene Proteins/*metabolism
MH  - RNA Processing, Post-Transcriptional
MH  - Transcription Factors/metabolism
EDAT- 2016/04/22 06:00
MHDA- 2017/07/25 06:00
CRDT- 2016/04/22 06:00
PHST- 2016/04/22 06:00 [entrez]
PHST- 2016/04/22 06:00 [pubmed]
PHST- 2017/07/25 06:00 [medline]
AID - 10.1039/c6mb00118a [doi]
PST - ppublish
SO  - Mol Biosyst. 2016 May 24;12(6):1922-8. doi: 10.1039/c6mb00118a.