PMID- 27098651
OWN - NLM
STAT- MEDLINE
DCOM- 20170209
LR  - 20181113
IS  - 1567-2387 (Electronic)
IS  - 1567-2379 (Linking)
VI  - 47
IP  - 3
DP  - 2016 Jun
TI  - Odontoblastic inductive potential of epithelial cells derived from human 
      deciduous dental pulp.
PG  - 345-51
LID - 10.1007/s10735-016-9676-1 [doi]
AB  - For the dentin regeneration, dental epithelial cells are indispensible and must 
      possess odontoblastic induction capability. Epithelial cell-like stem cells were 
      recently identified in human deciduous dental pulp (DPESCs). However, their 
      cellular characteristics remain poorly defined. The purpose of this study was to 
      characterize DPESCs compared to HAT-7 ameloblastic cells. Expression levels of 
      ameloblast-specific markers [odontogenic ameloblast-associated protein (Odam), 
      matrix metalloproteinase (Mmp)-20, amelogenin, and ameloblastin] were detected in 
      DPESCs. Co-culturing odontoblastic MDPC-23 cells with DPESCs increased expression 
      of odontoblast differentiation markers (Dmp1 and Dspp) from days 4 to 10, while 
      the expression of bone sialoprotein rapidly decreased. MDPC-23 cells cultured in 
      DPESC-conditioned medium (CM) showed increased Dspp promoter activity compared 
      with control MDPC-23 cultures. Mineralization was first observed in the CM groups 
      from day 4 and proceeded rapidly until day 14, whereas mineralized nodules were 
      found from day 7 in control media-cultured cells. In conclusion, DPESCs in human 
      deciduous pulp possess ameloblast-like characteristics and differentiation 
      properties, and substances derived from DPESCs promote odontoblastic 
      differentiation. Thus, our results indicate that DPESCs can be a realistic 
      epithelial source for use in odontoblastic induction and dentin formation of 
      dental mesenchymal cells.
FAU - Lee, Hye-Kyung
AU  - Lee HK
AD  - Department of Oral Histology-Developmental Biology and Dental Research Institute, 
      School of Dentistry, Seoul National University, 101 Daehagro, Chongro-gu, Seoul, 
      110-749, Republic of Korea.
FAU - Park, Ji-Won
AU  - Park JW
AD  - Department of Orthodontics, School of Dentistry, Seoul National University, 101 
      Daehagro, Chongro-gu, Seoul, 110-749, Republic of Korea.
FAU - Seo, You-Mi
AU  - Seo YM
AD  - Department of Oral Histology-Developmental Biology and Dental Research Institute, 
      School of Dentistry, Seoul National University, 101 Daehagro, Chongro-gu, Seoul, 
      110-749, Republic of Korea.
FAU - Kim, Ha Hoon
AU  - Kim HH
AD  - Department of Oral Histology-Developmental Biology and Dental Research Institute, 
      School of Dentistry, Seoul National University, 101 Daehagro, Chongro-gu, Seoul, 
      110-749, Republic of Korea.
FAU - Lee, Gene
AU  - Lee G
AD  - Laboratory of Molecular Genetics and Stem Cell Differentiation, Dental Research 
      Institute, School of Dentistry, Seoul National University, Seoul, 110-749, 
      Republic of Korea.
FAU - Bae, Hyun-Sook
AU  - Bae HS
AD  - Department of Dental Hygiene, Namseoul University, Cheonan, 331-707, Republic of 
      Korea.
FAU - Park, Joo-Cheol
AU  - Park JC
AD  - Department of Oral Histology-Developmental Biology and Dental Research Institute, 
      School of Dentistry, Seoul National University, 101 Daehagro, Chongro-gu, Seoul, 
      110-749, Republic of Korea. jcapark@snu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20160420
PL  - Netherlands
TA  - J Mol Histol
JT  - Journal of molecular histology
JID - 101193653
RN  - 0 (Biomarkers)
SB  - IM
MH  - Biomarkers
MH  - *Cell Differentiation/genetics
MH  - Cells, Cultured
MH  - Dental Pulp/*cytology
MH  - Epithelial Cells/*cytology/*metabolism
MH  - Gene Expression
MH  - Humans
MH  - Odontoblasts/*cytology/*metabolism
MH  - Promoter Regions, Genetic
MH  - Transcriptional Activation
OTO - NOTNLM
OT  - Deciduous pulp
OT  - Dental epithelial cell
OT  - Epithelial-mesenchymal interaction
OT  - Induction
OT  - Odontoblast
EDAT- 2016/04/22 06:00
MHDA- 2017/02/10 06:00
CRDT- 2016/04/22 06:00
PHST- 2016/03/09 00:00 [received]
PHST- 2016/04/15 00:00 [accepted]
PHST- 2016/04/22 06:00 [entrez]
PHST- 2016/04/22 06:00 [pubmed]
PHST- 2017/02/10 06:00 [medline]
AID - 10.1007/s10735-016-9676-1 [pii]
AID - 10.1007/s10735-016-9676-1 [doi]
PST - ppublish
SO  - J Mol Histol. 2016 Jun;47(3):345-51. doi: 10.1007/s10735-016-9676-1. Epub 2016 
      Apr 20.