PMID- 27103466
OWN - NLM
STAT- MEDLINE
DCOM- 20170324
LR  - 20210308
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 51
IP  - 3
DP  - 2016 Jun
TI  - Effects of Interferon-Tau and Steroids on Cytochrome P450 Activity in Bovine 
      Endometrial Epithelial Cells.
PG  - 415-20
LID - 10.1111/rda.12695 [doi]
AB  - The objective of the current study was to examine cyclooxygenase (COX), 
      cytochrome P450 1A (CYP1A) and 2C (CYP2C) activity in bovine endometrial cell 
      cultures following exposure to oxytocin (OT), interferon-tau (IFN), estradiol (E2) 
      and/or progesterone (P4). Bovine endometrial epithelial cells were treated with 
      OT, IFN, a combination of OT+IFN or control (CON) media for 24 h. For the second 
      experiment, cells were treated with E2, P4, a combination of E2 + P4 or CON media 
      for 24 h. Treatments were performed in triplicate, and the experiment was 
      repeated four times (n = 12 per treatment). Treatment with OT alone increased 
      (p < 0.01) activity of COX compared with CON; however, OT alone did not alter 
      activity of CYP1A (p = 0.55) or CYP2C (p = 0.46) compared with CON. Activity of 
      CYP1A and CYP2C was decreased in cells exposed to IFN (p < 0.01) or OT+IFN 
      (p < 0.01) compared with CON. Treatment with E2 alone did not alter activity of 
      CYP1A (p = 0.64) or CYP2C (p = 0.06) compared with CON. Activity of CYP1A and 
      CYP2C was decreased (p < 0.01) in P4 vs CON. In summary, IFN exposure, 
      irrespective of OT treatment, decreased the activity of CYP1A and CYP2C. Activity 
      of CYP1A was decreased following P4 treatment alone, while that of CYP2C was 
      decreased following both P4 and E2 + P4 treatment. The mixed function 
      monooxygenase enzymes, CYP1A and CYP2C, have been implicated in synthesizing 
      embryotoxic compounds; therefore, downregulation in the endometrium may be 
      necessary during maternal recognition of pregnancy.
CI  - (c) 2016 Blackwell Verlag GmbH.
FAU - Gilfeather, C L
AU  - Gilfeather CL
AD  - Department of Animal and Dairy Sciences, Mississippi State University, 
      Mississippi State, MS, USA.
FAU - Lemley, C O
AU  - Lemley CO
AD  - Department of Animal and Dairy Sciences, Mississippi State University, 
      Mississippi State, MS, USA.
LA  - eng
PT  - Journal Article
DEP - 20160422
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
RN  - 0 (Interferon Type I)
RN  - 0 (Pregnancy Proteins)
RN  - 0 (Steroids)
RN  - 0 (cytochrome P-450 CYP2C subfamily)
RN  - 0 (interferon tau)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 50-56-6 (Oxytocin)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - *Cattle
MH  - Cells, Cultured
MH  - Cytochrome P-450 CYP1A1/*metabolism
MH  - Cytochrome P-450 Enzyme System/*metabolism
MH  - Endometrium/drug effects/*enzymology
MH  - Epithelial Cells/drug effects/enzymology
MH  - Estradiol/pharmacology
MH  - Female
MH  - Interferon Type I/*pharmacology
MH  - Oxytocin/pharmacology
MH  - Pregnancy
MH  - Pregnancy Proteins/*pharmacology
MH  - Progesterone/pharmacology
MH  - Steroids/*pharmacology
EDAT- 2016/04/23 06:00
MHDA- 2017/03/25 06:00
CRDT- 2016/04/23 06:00
PHST- 2016/01/22 00:00 [received]
PHST- 2016/03/29 00:00 [accepted]
PHST- 2016/04/23 06:00 [entrez]
PHST- 2016/04/23 06:00 [pubmed]
PHST- 2017/03/25 06:00 [medline]
AID - 10.1111/rda.12695 [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2016 Jun;51(3):415-20. doi: 10.1111/rda.12695. Epub 2016 Apr 
      22.