PMID- 27107842 OWN - NLM STAT- MEDLINE DCOM- 20170731 LR - 20221207 IS - 1590-3729 (Electronic) IS - 0939-4753 (Linking) VI - 26 IP - 5 DP - 2016 May TI - The effect of a vegetarian versus conventional hypocaloric diet on serum concentrations of persistent organic pollutants in patients with type 2 diabetes. PG - 430-8 LID - S0939-4753(15)30248-9 [pii] LID - 10.1016/j.numecd.2016.01.008 [doi] AB - BACKGROUND AND AIMS: The aim of this study was to explore the effect of a vegetarian versus conventional diet on the serum levels of persistent organic pollutants (POPs) in patients with T2D after 12 weeks of dietary intervention and to assess their relationships with metabolic parameters. METHODS AND RESULTS: Men and women with T2D were randomly assigned to follow either a vegetarian diet without fish or meat (n = 37) or an isocaloric conventional antidiabetic diet (n = 37). Both diets were energy restricted (minus 500 kcal/day). All foods were provided to the participants. At randomization (week 0) and 12 weeks, the meal test was performed to assess the beta-cell function and serum levels of 24 POPs. Dioxins and dioxin-like POPs were analyzed by isotope dilution high-resolution gas chromatography (HRGC) and mass spectrometry after cleanup of the silica and carbon columns. Non-dioxin-like POPs were analyzed by gas chromatography with an electron capture detector (GC-ECD). Statistical analyses used were repeated-measures analysis of variance (ANOVA), a multivariate regression model, and Pearson's correlations. We observed a statistically nonsignificant trend toward increases in the serum levels of most POPs in response to both hypocaloric diets with no differences between groups. In the groups combined, the change in serum concentrations of total POPs was correlated to changes in HbA1c (r = +0.34; p < 0.01), fasting plasma glucose (r = +0.41; p < 0.01) levels, and beta-cell function measured as insulin secretion at a reference glucose level (r = -0.37; p < 0.01), independent of the changes in body weight and volume of visceral fat. CONCLUSION: Short-term hypocaloric vegetarian and conventional diets did not reduce the POP levels, possibly due to mobilization of fat stores. Our findings support the relationship between POPs and diabetes, especially beta-cell function. TRIAL REGISTRATION: ClinicalTrials.gov number, NCT00883038, completed. CI - Copyright (c) 2016 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University. Published by Elsevier B.V. All rights reserved. FAU - Kahleova, H AU - Kahleova H AD - Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic. Electronic address: hana.kahleova@gmail.com. FAU - Tonstad, S AU - Tonstad S AD - Department of Endocrinology, Morbid Obesity and Preventive Medicine, Oslo University Hospital, Oslo, Norway. FAU - Rosmus, J AU - Rosmus J AD - State Veterinary Institute Prague, Sidlistni 24, 165 03 Prague, Czech Republic. FAU - Fisar, P AU - Fisar P AD - State Veterinary Institute Prague, Sidlistni 24, 165 03 Prague, Czech Republic. FAU - Mari, A AU - Mari A AD - C.N.R. (National Research Council) Institute of Systems Science and Biomedical Engineering, Padua, Italy. FAU - Hill, M AU - Hill M AD - Institute of Endocrinology, Narodni 8, 11394 Prague, Czech Republic. FAU - Pelikanova, T AU - Pelikanova T AD - Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic. LA - eng SI - ClinicalTrials.gov/NCT00883038 PT - Comparative Study PT - Journal Article PT - Randomized Controlled Trial PT - Research Support, Non-U.S. Gov't DEP - 20160128 PL - Netherlands TA - Nutr Metab Cardiovasc Dis JT - Nutrition, metabolism, and cardiovascular diseases : NMCD JID - 9111474 RN - 0 (Biomarkers) RN - 0 (Blood Glucose) RN - 0 (Dioxins) RN - 0 (Environmental Pollutants) RN - 0 (Glycated Hemoglobin A) RN - 0 (Insulin) RN - 0 (hemoglobin A1c protein, human) SB - IM MH - Adiposity MH - Adult MH - Aged MH - Biomarkers/blood MH - Blood Glucose/metabolism MH - *Caloric Restriction MH - Czech Republic MH - Diabetes Mellitus, Type 2/blood/diagnosis/*diet therapy/physiopathology MH - *Diet, Reducing MH - *Diet, Vegetarian MH - Dioxins/adverse effects/*blood MH - Environmental Exposure MH - Environmental Pollutants/adverse effects/*blood MH - Female MH - *Food Contamination MH - Gas Chromatography-Mass Spectrometry MH - Glycated Hemoglobin/metabolism MH - Humans MH - Insulin/blood MH - Insulin-Secreting Cells/metabolism MH - Intra-Abdominal Fat/metabolism/physiopathology MH - Male MH - Middle Aged MH - Multivariate Analysis MH - Time Factors MH - Treatment Outcome MH - Weight Loss OTO - NOTNLM OT - Persistent organic pollutants OT - Type 2 diabetes OT - Vegetarian diet EDAT- 2016/04/25 06:00 MHDA- 2017/08/02 06:00 CRDT- 2016/04/25 06:00 PHST- 2015/10/01 00:00 [received] PHST- 2015/12/18 00:00 [revised] PHST- 2016/01/21 00:00 [accepted] PHST- 2016/04/25 06:00 [entrez] PHST- 2016/04/25 06:00 [pubmed] PHST- 2017/08/02 06:00 [medline] AID - S0939-4753(15)30248-9 [pii] AID - 10.1016/j.numecd.2016.01.008 [doi] PST - ppublish SO - Nutr Metab Cardiovasc Dis. 2016 May;26(5):430-8. doi: 10.1016/j.numecd.2016.01.008. Epub 2016 Jan 28.