PMID- 27108048
OWN - NLM
STAT- MEDLINE
DCOM- 20161214
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1447
DP  - 2016 May 20
TI  - Determination of amphetamines in hair by integrating sample disruption, clean-up 
      and solid phase derivatization.
PG  - 47-56
LID - S0021-9673(16)30459-9 [pii]
LID - 10.1016/j.chroma.2016.04.036 [doi]
AB  - The utility of matrix solid phase dispersion (MSPD) for the direct analysis of 
      amphetamines in hair samples has been evaluated, using liquid chromatography (LC) 
      with fluorescence detection and precolumn derivatization. The proposed approach 
      is based on the employment of MSPD for matrix disruption and clean-up, followed 
      by the derivatization of the analytes onto the dispersant-sample blend. The 
      fluorogenic reagent 9-fluorenylmethyl chloroformate (FMOC) has been used for 
      derivatization. Different conditions for MSPD, analyte purification and solid 
      phase derivatization have been tested, using amphetamine (AMP), methamphetamine 
      (MET), ephedrine (EPE) and 3,4-methylenedioxymethamphetamine (MDMA) as model 
      compounds. The results have been compared with those achieved by using 
      ultrasound-assisted alkaline digestion and by MSPD combined with conventional 
      solution derivatization. On the basis of the results obtained, a methodology is 
      proposed for the analysis of amphetamines in hair which integrates sample 
      disruption, clean-up and derivatization using a C18 phase. Improved sensitivity 
      is achieved with respect to that obtained by the alkaline digestion or by the 
      MSPD followed by solution derivatization methods. The method can be used for the 
      quantification of the tested amphetamines within the 2.0-20.0ng/mg concentration 
      interval, with limits of detection (LODs) of 0.25-0.75ng/mg. The methodology is 
      very simple and rapid (the preparation of the sample takes less than 15min).
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Argente-Garcia, A
AU  - Argente-Garcia A
AD  - Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 
      46100-Burjassot, Valencia, Spain.
FAU - Moliner-Martinez, Y
AU  - Moliner-Martinez Y
AD  - Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 
      46100-Burjassot, Valencia, Spain.
FAU - Campins-Falco, P
AU  - Campins-Falco P
AD  - Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 
      46100-Burjassot, Valencia, Spain.
FAU - Verdu-Andres, J
AU  - Verdu-Andres J
AD  - Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 
      46100-Burjassot, Valencia, Spain.
FAU - Herraez-Hernandez, R
AU  - Herraez-Hernandez R
AD  - Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 
      46100-Burjassot, Valencia, Spain. Electronic address: rosa.herraez@uv.es.
LA  - eng
PT  - Journal Article
DEP - 20160420
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Amphetamines)
RN  - 0 (Fluorenes)
RN  - 0 (Fluorescent Dyes)
RN  - 44RAL3456C (Methamphetamine)
RN  - 9PLB0BTT90 (1-(9-fluorenyl)methyl chloroformate)
RN  - CK833KGX7E (Amphetamine)
RN  - GN83C131XS (Ephedrine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Amphetamine/analysis
MH  - Amphetamines/*analysis
MH  - Chromatography, Liquid/methods
MH  - Ephedrine/analysis
MH  - Fluorenes/chemistry
MH  - Fluorescent Dyes/chemistry
MH  - Hair/*chemistry
MH  - Humans
MH  - Methamphetamine/analysis
MH  - N-Methyl-3,4-methylenedioxyamphetamine/analysis
OTO - NOTNLM
OT  - Amphetamines
OT  - Drug analysis
OT  - Hair
OT  - Liquid chromatography
OT  - Matrix-solid phase dispersion (MSPD)
OT  - Solid phase derivatization
EDAT- 2016/04/25 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/04/25 06:00
PHST- 2016/02/23 00:00 [received]
PHST- 2016/04/14 00:00 [revised]
PHST- 2016/04/14 00:00 [accepted]
PHST- 2016/04/25 06:00 [entrez]
PHST- 2016/04/25 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - S0021-9673(16)30459-9 [pii]
AID - 10.1016/j.chroma.2016.04.036 [doi]
PST - ppublish
SO  - J Chromatogr A. 2016 May 20;1447:47-56. doi: 10.1016/j.chroma.2016.04.036. Epub 
      2016 Apr 20.