PMID- 27108278
OWN - NLM
STAT- MEDLINE
DCOM- 20180117
LR  - 20180117
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 408
IP  - 25
DP  - 2016 Oct
TI  - Label-free electrochemiluminescence biosensor for ultrasensitive detection of 
      telomerase activity in HeLa cells based on extension reaction and intercalation 
      of Ru(phen)3 (2.).
PG  - 7105-11
LID - 10.1007/s00216-016-9561-5 [doi]
AB  - Telomerase is one of the most common markers of human malignant tumors, such as 
      uterine, stomach, esophageal, breast, colorectal, laryngeal squamous cell, 
      thyroid, bladder, and so on. It is necessary to develop some sensitive but 
      convenient detection methods for telomerase activity determination. In this 
      study, a label-free and ultrasensitive electrochemiluminescence (ECL) biosensor 
      has been fabricated to detect the activity of telomerase extracted from HeLa 
      cells. Thiolated telomerase substrate (TS) primer was immobilized on the gold 
      electrode surface through gold-sulfur (Au-S) interaction and then elongated by 
      telomerase specifically. Then, it was hybridized with complementary DNA to form 
      double-stranded DNA (dsDNA) fragments on the electrode surface, and Ru(phen)3 
      (2+) has been intercalated into the dsDNA grooves to act as the ECL probe. The 
      enhanced ECL intensity has a linear relationship with the number of HeLa cells in 
      the range of 5 approximately 5000 and with a detection limit of 2 HeLa cells. The proposed ECL 
      biosensor has high specificity to telomerase in the presence of common 
      interferents. The relative standard deviations (RSDs) were <5 % at 100 HeLa 
      cells. The proposed method provides a convenient approach for telomerase-related 
      cancer screening or diagnosis.
FAU - Lin, Yue
AU  - Lin Y
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, 
      Fuzhou University, Fuzhou, Fujian, 350116, China.
FAU - Yang, Linlin
AU  - Yang L
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, 
      Fuzhou University, Fuzhou, Fujian, 350116, China.
FAU - Yue, Guiyin
AU  - Yue G
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, 
      Fuzhou University, Fuzhou, Fujian, 350116, China.
FAU - Chen, Lifen
AU  - Chen L
AD  - State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the 
      Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian, 350002, China.
FAU - Qiu, Bin
AU  - Qiu B
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, 
      Fuzhou University, Fuzhou, Fujian, 350116, China.
FAU - Guo, Longhua
AU  - Guo L
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, 
      Fuzhou University, Fuzhou, Fujian, 350116, China.
FAU - Lin, Zhenyu
AU  - Lin Z
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, 
      Fuzhou University, Fuzhou, Fujian, 350116, China. zylin@fzu.edu.cn.
AD  - Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China. 
      zylin@fzu.edu.cn.
FAU - Chen, Guonan
AU  - Chen G
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, 
      Fuzhou University, Fuzhou, Fujian, 350116, China.
LA  - eng
PT  - Journal Article
DEP - 20160423
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Intercalating Agents)
RN  - 0 (Luminescent Agents)
RN  - 0 (Organometallic Compounds)
RN  - 0 (Phenanthrolines)
RN  - 22873-66-1 (tris-(1,10-phenanthroline)ruthenium)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Biosensing Techniques/methods
MH  - DNA/*metabolism
MH  - Enzyme Assays/methods
MH  - HeLa Cells
MH  - Humans
MH  - Intercalating Agents/*metabolism
MH  - Luminescent Agents/*metabolism
MH  - Luminescent Measurements/*methods
MH  - Organometallic Compounds/*metabolism
MH  - Phenanthrolines/*metabolism
MH  - Telomerase/*metabolism
OTO - NOTNLM
OT  - Electrochemiluminescence
OT  - HeLa cell
OT  - Label free
OT  - Telomerase
EDAT- 2016/04/25 06:00
MHDA- 2018/01/18 06:00
CRDT- 2016/04/25 06:00
PHST- 2016/02/02 00:00 [received]
PHST- 2016/04/12 00:00 [accepted]
PHST- 2016/03/31 00:00 [revised]
PHST- 2016/04/25 06:00 [entrez]
PHST- 2016/04/25 06:00 [pubmed]
PHST- 2018/01/18 06:00 [medline]
AID - 10.1007/s00216-016-9561-5 [pii]
AID - 10.1007/s00216-016-9561-5 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2016 Oct;408(25):7105-11. doi: 10.1007/s00216-016-9561-5. Epub 
      2016 Apr 23.