PMID- 27108284
OWN - NLM
STAT- MEDLINE
DCOM- 20180129
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 408
IP  - 17
DP  - 2016 Jul
TI  - Sequential SERS determination of aspirin and vitamin C using in situ 
      laser-induced photochemical silver substrate synthesis in a moving flow cell.
PG  - 4733-41
LID - 10.1007/s00216-016-9562-4 [doi]
AB  - In this contribution, the utility of sequential injection analysis in combination 
      with surface-enhanced Raman spectroscopy (SERS) as a detection technique was 
      investigated for simultaneous determination of aspirin and vitamin C in their 
      pharmaceutical dosage forms and in spiked urine samples. The silver substrate was 
      synthesized in situ by laser-induced photochemical procedure. By focusing the 
      laser on a flow cell at 1 ml/min of continuous flow of 0.5 mM silver nitrate and 
      5 mM sodium citrate mixture, an active silver spot on the inner wall of the flow 
      cell was prepared in a few seconds. The whole setup is fully computer controlled 
      using ATLAS software to combine the two techniques. The system allows sequential 
      determination of aspirin concentrations ranging from 100 to 500 ng/ml and vitamin 
      C concentrations between 10 and 110 ng/ml with good precision of relative 
      standard deviations (RSDs) of 0.85 and 1.7 %, respectively. A comparison of these 
      results with those of the reported procedures showed excellent results compared 
      with t and F values, indicating good accuracy and precision. The detection limits 
      were 32 and 3 ng/ml for aspirin and vitamin C, respectively.
FAU - El-Zahry, Marwa R
AU  - El-Zahry MR
AD  - Institute of Chemical Technologies and Analytics, Vienna University of 
      Technology, Getreidemarkt 9/151, 1060, Vienna, Austria. marwazahry@aun.edu.eg.
AD  - Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut 
      University, 71526, Assiut, Egypt. marwazahry@aun.edu.eg.
FAU - Refaat, Ibrahim H
AU  - Refaat IH
AD  - Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut 
      University, 71526, Assiut, Egypt.
FAU - Mohamed, Horria A
AU  - Mohamed HA
AD  - Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut 
      University, 71526, Assiut, Egypt.
FAU - Lendl, Bernhard
AU  - Lendl B
AD  - Institute of Chemical Technologies and Analytics, Vienna University of 
      Technology, Getreidemarkt 9/151, 1060, Vienna, Austria.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20160423
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 3M4G523W1G (Silver)
RN  - PQ6CK8PD0R (Ascorbic Acid)
RN  - R16CO5Y76E (Aspirin)
SB  - IM
MH  - Ascorbic Acid/*analysis
MH  - Aspirin/*analysis
MH  - Humans
MH  - Limit of Detection
MH  - Microscopy, Electron, Scanning
MH  - *Photochemistry
MH  - Silver/*chemistry
OTO - NOTNLM
OT  - Aspirin
OT  - In situ silver photoreduction
OT  - SERS
OT  - Sequential injection analysis
OT  - Vitamin C
EDAT- 2016/04/25 06:00
MHDA- 2018/01/30 06:00
CRDT- 2016/04/25 06:00
PHST- 2016/02/28 00:00 [received]
PHST- 2016/04/12 00:00 [accepted]
PHST- 2016/04/06 00:00 [revised]
PHST- 2016/04/25 06:00 [entrez]
PHST- 2016/04/25 06:00 [pubmed]
PHST- 2018/01/30 06:00 [medline]
AID - 10.1007/s00216-016-9562-4 [pii]
AID - 10.1007/s00216-016-9562-4 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2016 Jul;408(17):4733-41. doi: 10.1007/s00216-016-9562-4. Epub 
      2016 Apr 23.