PMID- 27108739
OWN - NLM
STAT- MEDLINE
DCOM- 20180709
LR  - 20181202
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 8
IP  - 19
DP  - 2016 May 18
TI  - Modulation of Protein Adsorption and Cell Proliferation on Polyethylene 
      Immobilized Graphene Oxide Reinforced HDPE Bionanocomposites.
PG  - 11954-68
LID - 10.1021/acsami.6b00946 [doi]
AB  - The uniform dispersion of nanoparticles in a polymer matrix, together with an 
      enhancement of interfacial adhesion is indispensable toward achieving better 
      mechanical properties in the nanocomposites. In the context to biomedical 
      applications, the type and amount of nanoparticles can potentially influence the 
      biocompatibility. To address these issues, we prepared high-density polyethylene 
      (HDPE) based composites reinforced with graphene oxide (GO) by melt mixing 
      followed by compression molding. In an attempt to tailor the dispersion and to 
      improve the interfacial adhesion, we immobilized polyethylene (PE) onto GO sheets 
      by nucleophilic addition-elimination reaction. A good combination of yield 
      strength (ca. 20 MPa), elastic modulus (ca. 600 MPa), and an outstanding 
      elongation at failure (ca. 70%) were recorded with 3 wt % polyethylene grafted 
      graphene oxide (PE-g-GO) reinforced HDPE composites. Considering the relevance of 
      protein adsorption as a biophysical precursor to cell adhesion, the protein 
      adsorption isotherms of bovine serum albumin (BSA) were determined to realize 
      three times higher equilibrium constant (Keq) for PE-g-GO-reinforced HDPE 
      composites as compared to GO-reinforced composites. To assess the 
      cytocompatibility, we grew osteoblast cell line (MC3T3) and human mesenchymal 
      stem cells (hMSCs) on HDPE/GO and HDPE/PE-g-GO composites, in vitro. The 
      statistically significant increase in metabolically active cell over different 
      time periods in culture for up to 6 days in MC3T3 and 7 days for hMSCs was 
      observed, irrespective of the substrate composition. Such observation indicated 
      that HDPE with GO or PE-g-GO addition (up to 3 wt %) can be used as cell growth 
      substrate. The extensive proliferation of cells with oriented growth pattern also 
      supported the fact that tailored GO addition can support cellular functionality 
      in vitro. Taken together, the experimental results suggest that the PE-g-GO in 
      HDPE can effectively be utilized to enhance both mechanical and cytocompatibility 
      properties and can further be explored for potential biomedical applications.
FAU - Upadhyay, Rahul
AU  - Upadhyay R
AD  - Laboratory for Biomaterials, Materials Research Center, Indian Institute of 
      Science ,Bangalore 560012, India.
FAU - Naskar, Sharmistha
AU  - Naskar S
AD  - Center for Biosystems Science and Engineering, Indian Institute of Science , 
      Bangalore 560012, India.
FAU - Bhaskar, Nitu
AU  - Bhaskar N
AD  - Laboratory for Biomaterials, Materials Research Center, Indian Institute of 
      Science ,Bangalore 560012, India.
FAU - Bose, Suryasarathi
AU  - Bose S
AD  - Department of Materials Engineering, Indian Institute of Science , Bangalore 
      560012, India.
FAU - Basu, Bikramjit
AU  - Basu B
AD  - Laboratory for Biomaterials, Materials Research Center, Indian Institute of 
      Science ,Bangalore 560012, India.
AD  - Center for Biosystems Science and Engineering, Indian Institute of Science , 
      Bangalore 560012, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160503
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 7782-42-5 (Graphite)
RN  - 9002-88-4 (Polyethylene)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Line
MH  - Cell Proliferation/*drug effects
MH  - *Graphite/chemistry/pharmacology
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Mice
MH  - Nanocomposites/*chemistry
MH  - Osteoblasts/cytology/*metabolism
MH  - *Polyethylene/chemistry/pharmacology
MH  - Serum Albumin, Bovine/*chemistry
OTO - NOTNLM
OT  - HDPE
OT  - cytocompatibility
OT  - grafting
OT  - graphene oxide
OT  - hMSC
OT  - nanocomposites
OT  - osteoblast
OT  - toxicity
EDAT- 2016/04/26 06:00
MHDA- 2018/07/10 06:00
CRDT- 2016/04/26 06:00
PHST- 2016/04/26 06:00 [entrez]
PHST- 2016/04/26 06:00 [pubmed]
PHST- 2018/07/10 06:00 [medline]
AID - 10.1021/acsami.6b00946 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2016 May 18;8(19):11954-68. doi: 
      10.1021/acsami.6b00946. Epub 2016 May 3.