PMID- 27110069
OWN - NLM
STAT- MEDLINE
DCOM- 20170117
LR  - 20240325
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2016
DP  - 2016
TI  - Arginine Relieves the Inflammatory Response and Enhances the Casein Expression in 
      Bovine Mammary Epithelial Cells Induced by Lipopolysaccharide.
PG  - 9618795
LID - 10.1155/2016/9618795 [doi]
LID - 9618795
AB  - As one of functional active amino acids, L-arginine holds a key position in 
      immunity. However, the mechanism that arginine modulates cow mammary inflammatory 
      response in ruminant is unclear. Therefore, this study was conducted to 
      investigate the effects of L-arginine on inflammatory response and casein 
      expression after challenging the bovine mammary epithelial cells (BMECs) with 
      lipopolysaccharide (LPS). The cells were divided into four groups, stimulated 
      with or without LPS (10 mug/mL) and treated with or without arginine (100 mug/mL) 
      for 12 h. The concentration of proinflammatory cytokines, inducible nitric oxide 
      synthase (iNOS), mammalian target of rapamycin (mTOR), and Toll-like receptor 4 
      (TLR4) signaling pathways as well as the casein was determined. The results 
      showed that arginine reduced the LPS-induced production like IL-1beta, IL-6, TNF-alpha, 
      and iNOS. Though the expression of NF-kappaB was attenuated and the mTOR signaling 
      pathway was upregulated, arginine had no effect on TLR4 expression. In addition, 
      our results show that the content of beta-casein and the total casein were enhanced 
      after arginine was supplemented in LPS-induced BMECs. In conclusion, arginine 
      could relieve the inflammatory reaction induced by LPS and enhance the 
      concentration of beta-casein and the total casein in bovine mammary epithelial 
      cells.
FAU - Wu, Tianyou
AU  - Wu T
AD  - Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of 
      Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
FAU - Wang, Chao
AU  - Wang C
AD  - Cell Signaling Group, School of Pathology and Laboratory Medicine, The University 
      of Western Australia, M Block QEII Medical Center, Monash Avenue, Nedlands, WA 
      6009, Australia.
FAU - Ding, Luoyang
AU  - Ding L
AD  - Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of 
      Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
FAU - Shen, Yizhao
AU  - Shen Y
AD  - Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of 
      Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
FAU - Cui, Huihui
AU  - Cui H
AD  - Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of 
      Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
FAU - Wang, Mengzhi
AU  - Wang M
AD  - Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of 
      Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
FAU - Wang, Hongrong
AU  - Wang H
AD  - Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of 
      Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160323
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (Caseins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Nitrogen Oxides)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Transcription Factor RelA)
RN  - 94ZLA3W45F (Arginine)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
SB  - IM
MH  - Animals
MH  - Arginine/*pharmacology/*therapeutic use
MH  - Caseins/*metabolism
MH  - Cattle
MH  - Epithelial Cells/drug effects/*metabolism
MH  - Humans
MH  - Inflammation/*drug therapy/*metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - Mammary Glands, Human/*cytology
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - Nitrogen Oxides/metabolism
MH  - Toll-Like Receptor 4/metabolism
MH  - Transcription Factor RelA/metabolism
PMC - PMC4821974
EDAT- 2016/04/26 06:00
MHDA- 2017/01/18 06:00
PMCR- 2016/03/23
CRDT- 2016/04/26 06:00
PHST- 2015/11/11 00:00 [received]
PHST- 2016/02/24 00:00 [accepted]
PHST- 2016/04/26 06:00 [entrez]
PHST- 2016/04/26 06:00 [pubmed]
PHST- 2017/01/18 06:00 [medline]
PHST- 2016/03/23 00:00 [pmc-release]
AID - 10.1155/2016/9618795 [doi]
PST - ppublish
SO  - Mediators Inflamm. 2016;2016:9618795. doi: 10.1155/2016/9618795. Epub 2016 Mar 
      23.