PMID- 27110767 OWN - NLM STAT- MEDLINE DCOM- 20170210 LR - 20240325 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 17 IP - 4 DP - 2016 Apr 21 TI - Characterization of Stripe Rust Resistance Genes in the Wheat Cultivar Chuanmai45. LID - 10.3390/ijms17040601 [doi] LID - 601 AB - The objective of this research was to characterize the high level of resistance to stripe that has been observed in the released wheat cultivar, Chuanmai45. A combination of classic genetic analysis, molecular and cytogenetic methods were used to characterize resistance in an F(2) population derived from Chuanmai45 and the susceptible Chuanmai42. Inheritance of resistance was shown to be conferred by two genes in Chuanmai45. Fluorescence in situ hybridization (FISH) was used along with segregation studies to show that one gene was located on a 1RS.1BL translocation. Molecular markers were employed to show that the other locus was located on chromosome 4B. The defeated gene, Yr24/26, on chromosome 1BL was present in the susceptible parent and lines that recombined this gene with the 1RS.1BL translocation were identified. The germplasm, loci, and associated markers identified in this study will be useful for application in breeding programs utilizing marker-assisted selection. FAU - Yang, Ennian AU - Yang E AD - Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. yangennian@126.com. FAU - Li, Guangrong AU - Li G AD - School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. ligr28@uestc.edu.cn. FAU - Li, Liping AU - Li L AD - Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. anzerna@126.com. FAU - Zhang, Zhenyu AU - Zhang Z AD - Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. zhzhyu1982@163.com. FAU - Yang, Wuyun AU - Yang W AD - Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. yangwuyun@126.com. FAU - Peng, Yunliang AU - Peng Y AD - Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. pengyunliang@yahoo.com. FAU - Zhu, Yongqing AU - Zhu Y AD - Institute of Agro Products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. zhuyongqing68@sina.com. FAU - Yang, Zujun AU - Yang Z AD - School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. yangzujun@uestc.edu.cn. FAU - Rosewarne, Garry M AU - Rosewarne GM AD - Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. garry.rosewarne@ecodev.vic.gov.au. AD - International Maize and Wheat Improvement Centre (CIMMYT), Apdo. Postal 6-6-41, Mexico 06600, D.F., Mexico. garry.rosewarne@ecodev.vic.gov.au. AD - Department of Environment and Primary Industries, 110 Natimuk Rd, Horsham, Victoria 3401, Australia. garry.rosewarne@ecodev.vic.gov.au. LA - eng PT - Journal Article DEP - 20160421 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Biomarkers) SB - IM MH - Biomarkers/metabolism MH - Disease Resistance/*genetics MH - *Genes, Plant MH - In Situ Hybridization MH - Karyotype MH - Plant Diseases/*microbiology MH - Translocation, Genetic MH - Triticum/*genetics PMC - PMC4849054 OTO - NOTNLM OT - Chuanmai45 OT - stripe rust resistance OT - wheat-rye 1RS.1BL translocations EDAT- 2016/04/26 06:00 MHDA- 2017/02/12 06:00 PMCR- 2016/04/01 CRDT- 2016/04/26 06:00 PHST- 2015/12/24 00:00 [received] PHST- 2016/04/14 00:00 [revised] PHST- 2016/04/15 00:00 [accepted] PHST- 2016/04/26 06:00 [entrez] PHST- 2016/04/26 06:00 [pubmed] PHST- 2017/02/12 06:00 [medline] PHST- 2016/04/01 00:00 [pmc-release] AID - ijms17040601 [pii] AID - ijms-17-00601 [pii] AID - 10.3390/ijms17040601 [doi] PST - epublish SO - Int J Mol Sci. 2016 Apr 21;17(4):601. doi: 10.3390/ijms17040601.