PMID- 27111895
OWN - NLM
STAT- MEDLINE
DCOM- 20170327
LR  - 20221207
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 4
DP  - 2016
TI  - Association of DPP4 Gene Polymorphisms with Type 2 Diabetes Mellitus in Malaysian 
      Subjects.
PG  - e0154369
LID - 10.1371/journal.pone.0154369 [doi]
LID - e0154369
AB  - BACKGROUND: Genetic polymorphisms of the Dipeptidyl Peptidase 4 (DPP4) gene may 
      play a role in the etiology of type 2 diabetes mellitus (T2DM). This study aimed 
      to investigate the possible association of single nucleotide polymorphisms (SNPs) 
      of the DPP4 gene in Malaysian subjects with T2DM and evaluated whether they had 
      an effect on the serum levels of soluble dipeptidyl peptidase 4 (sDPP-IV). 
      METHOD: Ten DPP4 SNPs were genotyped by TaqMan genotyping assays in 314 subjects 
      with T2DM and 235 controls. Of these, 71 metabolic syndrome (MetS) subjects were 
      excluded from subsequent analysis. The odds ratios (ORs) and their 95% confidence 
      interval (CIs) were calculated using multiple logistic regression for the 
      association between the SNPs of DPP4 and T2DM. In addition, the serum levels of 
      sDPP-IV were investigated to evaluate the association of the SNPs of DPP4 with 
      the sDPP-IV levels. RESULTS: Dominant, recessive, and additive genetic models 
      were employed to test the association of DPP4 polymorphisms with T2DM, after 
      adjusting for age, race, gender and BMI. The rs12617656 was associated with T2DM 
      in Malaysian subjects in the recessive genetic model (OR = 1.98, p = 0.006), 
      dominant model (OR = 1.95, p = 0.008), and additive model (OR = 1.63, p = 0.001). 
      This association was more pronounced among Malaysian Indians, recessive (OR = 
      3.21, p = 0.019), dominant OR = 3.72, p = 0.003) and additive model (OR = 2.29, p 
      = 0.0009). The additive genetic model showed that DPP4 rs4664443 and rs7633162 
      polymorphisms were associated with T2DM (OR = 1.53, p = 0.039), and (OR = 1.42, p 
      = 0.020), respectively. In addition, the rs4664443 G>A polymorphism was 
      associated with increased sDPP-IV levels (p = 0.042) in T2DM subjects. 
      CONCLUSIONS: DPP4 polymorphisms were associated with T2DM in Malaysian subjects, 
      and linked to variations in sDPP-IV levels. In addition, these associations were 
      more pronounced among Malaysian Indian subjects.
FAU - Ahmed, Radwan H
AU  - Ahmed RH
AD  - Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 
      Kuala Lumpur, Malaysia.
FAU - Huri, Hasniza Zaman
AU  - Huri HZ
AD  - Department of Pharmacy, Faculty of Medicine, University of Malaya, Kuala Lumpur, 
      Malaysia.
AD  - Clinical Investigation Centre, University Malaya Medical Centre, Kuala Lumpur, 
      Malaysia.
FAU - Al-Hamodi, Zaid
AU  - Al-Hamodi Z
AD  - Department of Biochemistry and Molecular Biology, Faculty of Medicine, Sana'a 
      University, Sana'a, Yemen.
FAU - Salem, Sameer D
AU  - Salem SD
AD  - Department of Biochemistry and Molecular Biology, Faculty of Medicine, Sana'a 
      University, Sana'a, Yemen.
FAU - Al-Absi, Boshra
AU  - Al-Absi B
AD  - Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 
      Kuala Lumpur, Malaysia.
FAU - Muniandy, Sekaran
AU  - Muniandy S
AD  - Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 
      Kuala Lumpur, Malaysia.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20160425
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (CTLA-4 Antigen)
RN  - 0 (CTLA4 protein, human)
RN  - EC 3.4.14.5 (DPP4 protein, human)
RN  - EC 3.4.14.5 (Dipeptidyl Peptidase 4)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Alleles
MH  - Asian People
MH  - CTLA-4 Antigen/genetics
MH  - Child
MH  - Child, Preschool
MH  - Diabetes Mellitus, Type 2/*ethnology/*genetics
MH  - Dipeptidyl Peptidase 4/*genetics
MH  - Female
MH  - Genotype
MH  - Graves Disease/epidemiology/genetics
MH  - Haplotypes
MH  - Hashimoto Disease/epidemiology/genetics
MH  - Humans
MH  - Malaysia
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Genetic
MH  - *Polymorphism, Single Nucleotide
MH  - Young Adult
PMC - PMC4844141
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/04/26 06:00
MHDA- 2017/03/28 06:00
PMCR- 2016/04/25
CRDT- 2016/04/26 06:00
PHST- 2016/01/20 00:00 [received]
PHST- 2016/04/12 00:00 [accepted]
PHST- 2016/04/26 06:00 [entrez]
PHST- 2016/04/26 06:00 [pubmed]
PHST- 2017/03/28 06:00 [medline]
PHST- 2016/04/25 00:00 [pmc-release]
AID - PONE-D-16-02705 [pii]
AID - 10.1371/journal.pone.0154369 [doi]
PST - epublish
SO  - PLoS One. 2016 Apr 25;11(4):e0154369. doi: 10.1371/journal.pone.0154369. 
      eCollection 2016.