PMID- 27113901
OWN - NLM
STAT- MEDLINE
DCOM- 20180903
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Apr 26
TI  - Amphiregulin mediates hCG-induced StAR expression and progesterone production in 
      human granulosa cells.
PG  - 24917
LID - 10.1038/srep24917 [doi]
LID - 24917
AB  - Progesterone plays critical roles in maintaining a successful pregnancy at the 
      early embryonic stage. Human chorionic gonadotropin (hCG) rapidly induces 
      amphiregulin (AREG) expression. However, it remains unknown whether AREG mediates 
      hCG-induced progesterone production. Thus, the objective of this study was to 
      investigate the role of AREG in hCG-induced progesterone production and the 
      underlying molecular mechanism in human granulosa cells; primary cells were used 
      as the experimental model. We demonstrated that the inhibition of EGFR and the 
      knockdown of AREG abolished hCG-induced steroidogenic acute regulatory protein 
      (StAR) expression and progesterone production. Importantly, follicular fluid AREG 
      levels were positively correlated with progesterone levels in the follicular 
      fluid and serum. Treatment with AREG increased StAR expression and progesterone 
      production, and these stimulatory effects were abolished by EGFR inhibition. 
      Moreover, activation of ERK1/2, but not PI3K/Akt, signaling was required for the 
      AREG-induced up-regulation of StAR expression and progesterone production. Our 
      results demonstrate that AREG mediates hCG-induced StAR expression and 
      progesterone production in human granulosa cells, providing novel evidence for 
      the role of AREG in the regulation of steroidogenesis.
FAU - Fang, Lanlan
AU  - Fang L
AD  - Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, 450052 China.
FAU - Yu, Yiping
AU  - Yu Y
AD  - Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, 450052 China.
FAU - Zhang, Ruizhe
AU  - Zhang R
AD  - Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, 450052 China.
FAU - He, Jingyan
AU  - He J
AD  - Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, 450052 China.
FAU - Sun, Ying-Pu
AU  - Sun YP
AD  - Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, 450052 China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160426
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Amphiregulin)
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (steroidogenic acute regulatory protein)
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Adult
MH  - Amphiregulin/genetics/*metabolism/pharmacology
MH  - Cells, Cultured
MH  - Chorionic Gonadotropin/*pharmacology
MH  - Female
MH  - Fertilization in Vitro
MH  - Follicular Fluid/chemistry
MH  - Gene Expression Regulation
MH  - Granulosa Cells/drug effects/*metabolism
MH  - Humans
MH  - Phosphoproteins/*metabolism
MH  - Progesterone/*biosynthesis
MH  - RNA, Small Interfering/genetics
MH  - Signal Transduction
PMC - PMC4845069
EDAT- 2016/04/27 06:00
MHDA- 2018/09/04 06:00
PMCR- 2016/04/26
CRDT- 2016/04/27 06:00
PHST- 2016/01/20 00:00 [received]
PHST- 2016/04/07 00:00 [accepted]
PHST- 2016/04/27 06:00 [entrez]
PHST- 2016/04/27 06:00 [pubmed]
PHST- 2018/09/04 06:00 [medline]
PHST- 2016/04/26 00:00 [pmc-release]
AID - srep24917 [pii]
AID - 10.1038/srep24917 [doi]
PST - epublish
SO  - Sci Rep. 2016 Apr 26;6:24917. doi: 10.1038/srep24917.