PMID- 27119058
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160427
LR  - 20201001
IS  - 2193-1801 (Print)
IS  - 2193-1801 (Electronic)
IS  - 2193-1801 (Linking)
VI  - 5
DP  - 2016
TI  - Identification of IgE-binding peptide and critical amino acids of Jatropha curcas 
      allergen involved in allergenic response.
PG  - 454
LID - 10.1186/s40064-016-2036-5 [doi]
LID - 454
AB  - Increasing energy demand has spurred interest in the use of biofuels. Jatropha 
      curcas (physic nut), an inedible oilseed, is a potential source of bioenergy. The 
      seeds, however, contain allergens such as Jat c 1, a 2S albumin that can induce 
      hypersensitivity reactions in humans and result in allergic diseases. Recent 
      advances in identifying and characterizing plant allergens and, in particular, 
      their immunoglobulin E (IgE)-binding epitopes have produced a wealth of 
      information. We identified IgE-binding regions and the critical amino acids 
      involved in the degranulation of mast cells and the release of histamine, 
      preliminary steps for the prevention and treatment of this allergy. Four 
      IgE-binding regions were identified in the sequence of Jat c 1. We identified and 
      demonstrated the fundamental role of two glutamic acid residues in IgE binding. 
      The sequence LEKQLEEGEVGS produces a random loop on the most exposed part of Jat 
      c 1. This region is important to the stimulation of the allergic response. The 
      possibility of using this information to produce vaccines and other 
      pharmacological agents for allergy treatment is discussed.
FAU - Crespo, Livia Maia
AU  - Crespo LM
AD  - Laboratorio de Quimica e Funcao de Proteinas e Peptideos, Centro de Biociencias e 
      Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. 
      Alberto Lamego, 2000, Parque California, Rio de Janeiro, CEP 28013-602 Brazil.
FAU - de Oliveira, Natalia Deus
AU  - de Oliveira ND
AD  - Laboratorio de Quimica e Funcao de Proteinas e Peptideos, Centro de Biociencias e 
      Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. 
      Alberto Lamego, 2000, Parque California, Rio de Janeiro, CEP 28013-602 Brazil.
FAU - Damatta, Renato Augusto
AU  - Damatta RA
AD  - Laboratorio de Biologia Celular e Tecidual, Centro de Biociencias e 
      Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Rio de 
      Janeiro, CEP 28013-602 Brazil.
FAU - do Nascimento, Viviane Veiga
AU  - do Nascimento VV
AD  - Laboratorio de Quimica e Funcao de Proteinas e Peptideos, Centro de Biociencias e 
      Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. 
      Alberto Lamego, 2000, Parque California, Rio de Janeiro, CEP 28013-602 Brazil.
FAU - Soares, Thais Pacheco
AU  - Soares TP
AD  - Laboratorio de Quimica e Funcao de Proteinas e Peptideos, Centro de Biociencias e 
      Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. 
      Alberto Lamego, 2000, Parque California, Rio de Janeiro, CEP 28013-602 Brazil.
FAU - Machado, Olga Lima Tavares
AU  - Machado OL
AD  - Laboratorio de Quimica e Funcao de Proteinas e Peptideos, Centro de Biociencias e 
      Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. 
      Alberto Lamego, 2000, Parque California, Rio de Janeiro, CEP 28013-602 Brazil.
LA  - eng
PT  - Journal Article
DEP - 20160414
PL  - Switzerland
TA  - Springerplus
JT  - SpringerPlus
JID - 101597967
PMC - PMC4831952
OTO - NOTNLM
OT  - 2S albumin
OT  - Allergen
OT  - Biofuel
OT  - IgE-binding peptide
OT  - Jatropha curcas
EDAT- 2016/04/28 06:00
MHDA- 2016/04/28 06:01
PMCR- 2016/04/14
CRDT- 2016/04/28 06:00
PHST- 2016/02/03 00:00 [received]
PHST- 2016/03/20 00:00 [accepted]
PHST- 2016/04/28 06:00 [entrez]
PHST- 2016/04/28 06:00 [pubmed]
PHST- 2016/04/28 06:01 [medline]
PHST- 2016/04/14 00:00 [pmc-release]
AID - 2036 [pii]
AID - 10.1186/s40064-016-2036-5 [doi]
PST - epublish
SO  - Springerplus. 2016 Apr 14;5:454. doi: 10.1186/s40064-016-2036-5. eCollection 
      2016.