PMID- 27119088
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20190723
IS  - 2314-6753 (Electronic)
IS  - 2314-6745 (Print)
VI  - 2016
DP  - 2016
TI  - Combined Microencapsulated Islet Transplantation and Revascularization of 
      Aortorenal Bypass in a Diabetic Nephropathy Rat Model.
PG  - 9706321
LID - 10.1155/2016/9706321 [doi]
LID - 9706321
AB  - OBJECTIVE: Revascularization of aortorenal bypass is a preferred technique for 
      renal artery stenosis (RAS) in diabetic nephropathy (DN) patients. Restenosis of 
      graft vessels also should be considered in patients lacking good control of blood 
      glucose. In this study, we explored a combined strategy to prevent the recurrence 
      of RAS in the DN rat model. METHODS: A model of DN was established by 
      intraperitoneal injection of streptozotocin. Rats were divided into 4 groups: SR 
      group, MIT group, Com group, and the untreated group. The levels of blood glucose 
      and urine protein were measured, and changes in renal pathology were observed. 
      The expression of monocyte chemoattractant protein-1 (MCP-1) in graft vessels was 
      assessed by immunohistochemical staining. Histopathological staining was 
      performed to assess the pathological changes of glomeruli and tubules. RESULTS: 
      The levels of urine protein and the expression of MCP-1 in graft vessels were 
      decreased after islet transplantation. The injury of glomerular basement membrane 
      and podocytes was significantly ameliorated. CONCLUSIONS: The combined strategy 
      of revascularization and microencapsulated islet transplantation had multiple 
      protective effects on diabetic nephropathy, including preventing atherosclerosis 
      in the graft vessels and alleviating injury to the glomerular filtration barrier. 
      This combined strategy may be helpful for DN patients with RAS.
FAU - He, Yunqiang
AU  - He Y
AD  - Zhejiang Provincial Top Key Discipline in Surgery, Wenzhou Key Laboratory of 
      Surgery, Department of Surgery, The First Affiliated Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, China.
FAU - Xu, Ziqiang
AU  - Xu Z
AD  - Department of Transplantation, The First Affiliated Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, China.
FAU - Fu, Hongxing
AU  - Fu H
AD  - School of Pharmacy, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China.
FAU - Chen, Bin
AU  - Chen B
AD  - Department of B-Mode Ultrasound, The First Affiliated Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, China.
FAU - Wang, Silu
AU  - Wang S
AD  - Zhejiang Provincial Top Key Discipline in Surgery, Wenzhou Key Laboratory of 
      Surgery, Department of Surgery, The First Affiliated Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, China.
FAU - Chen, Bicheng
AU  - Chen B
AD  - Zhejiang Provincial Top Key Discipline in Surgery, Wenzhou Key Laboratory of 
      Surgery, Department of Surgery, The First Affiliated Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, China.
FAU - Zhou, Mengtao
AU  - Zhou M
AD  - Zhejiang Provincial Top Key Discipline in Surgery, Wenzhou Key Laboratory of 
      Surgery, Department of Surgery, The First Affiliated Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, China.
FAU - Cai, Yong
AU  - Cai Y
AD  - Department of Transplantation, The First Affiliated Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160328
PL  - England
TA  - J Diabetes Res
JT  - Journal of diabetes research
JID - 101605237
RN  - 0 (Biomarkers)
RN  - 0 (Blood Glucose)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Anastomosis, Surgical
MH  - Animals
MH  - Aorta, Abdominal/metabolism/pathology/*surgery
MH  - Biomarkers/blood
MH  - Blood Glucose/metabolism
MH  - Chemokine CCL2/metabolism
MH  - Diabetes Mellitus, Experimental/complications
MH  - Diabetic Angiopathies/blood/etiology/*prevention & control
MH  - Diabetic Nephropathies/blood/etiology/*surgery
MH  - Glomerular Filtration Barrier/physiopathology
MH  - Glomerular Filtration Rate
MH  - *Islets of Langerhans Transplantation
MH  - Male
MH  - Proteinuria/etiology/physiopathology/surgery
MH  - Rats, Sprague-Dawley
MH  - Renal Artery/metabolism/pathology/*surgery
MH  - Renal Artery Obstruction/blood/etiology/*prevention & control
MH  - Time Factors
MH  - *Vascular Surgical Procedures
PMC - PMC4826923
EDAT- 2016/04/28 06:00
MHDA- 2016/12/15 06:00
PMCR- 2016/03/28
CRDT- 2016/04/28 06:00
PHST- 2015/11/18 00:00 [received]
PHST- 2016/03/08 00:00 [accepted]
PHST- 2016/04/28 06:00 [entrez]
PHST- 2016/04/28 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2016/03/28 00:00 [pmc-release]
AID - 10.1155/2016/9706321 [doi]
PST - ppublish
SO  - J Diabetes Res. 2016;2016:9706321. doi: 10.1155/2016/9706321. Epub 2016 Mar 28.