PMID- 27121152
OWN - NLM
STAT- MEDLINE
DCOM- 20170118
LR  - 20210208
IS  - 1097-0339 (Electronic)
IS  - 1097-0339 (Linking)
VI  - 44
IP  - 7
DP  - 2016 Jul
TI  - BAP1 Immunostain and CDKN2A (p16) FISH Analysis: Clinical Applicability for the 
      Diagnosis of Malignant Mesothelioma in Effusions.
PG  - 599-606
LID - 10.1002/dc.23491 [doi]
AB  - BACKGROUND: Loss of BAP1 by immunohistochemistry (IHC) and CDKN2A(p16) deletion 
      by fluorescence in situ hybridization (FISH) have been proposed to distinguish 
      malignant mesothelioma (MM) from atypical reactive mesothelial proliferations 
      (ARMP) in effusions but it is uncertain whether both tests are needed routinely. 
      METHODS: Paraffin embedded blocks from 67 effusions (32 MM, 35 ARMP) were 
      evaluated with BAP1 IHC. 38 of them (17 MM, 21 ARMP) were also analyzed with 
      CDKN2A (p16) FISH. Criteria for MM were absence of BAP1 nuclear staining in >50% 
      of atypical mesothelial cells in the presence of a positive internal control, 
      and/or CDKN2A(p16) homozygous or hemizygous deletion pattern in >15% or >41.5% of 
      atypical mesothelial cells, respectively. Sensitivity (SS), specificity (SP), 
      positive, and negative predictive values (PPV, NPV) for MM were calculated. 
      RESULTS: 17 of 32 MM were correctly diagnosed by IHC (SS 53.1%, SP 85.7%, PPV 
      77.3%, NPV 64.3% for MM). In the subset of 38 cases studied by IHC and FISH, the 
      SS, SP, PPV, and NPV of IHC for MM were 41.2, 81.0, 63.6, and 63.0%, 
      respectively. 7 of 17 MM were diagnosed by FISH (SS 41.2%, SP 100%, PPV 100%, NPV 
      67.7% for MM). CONCLUSION: BAP1 IHC and CDKN2A(p16) FISH yield similar 
      sensitivities for MM in paired samples but FISH has a higher specificity. To 
      diagnose effusions with atypical mesothelial cells, use of both tests is optimal. 
      However, BAP1 IHC alone may be sufficient to diagnose MM when clinical, imaging, 
      and cytological findings strongly suggest MM. Diagn. Cytopathol. 2016. (c) 2016 
      Wiley Periodicals, Inc. Diagn. Cytopathol. 2016;44:599-606. (c) 2016 Wiley 
      Periodicals, Inc.
CI  - (c) 2016 Wiley Periodicals, Inc.
FAU - Walts, Ann E
AU  - Walts AE
AD  - Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los 
      Angeles, California.
FAU - Hiroshima, Kenzo
AU  - Hiroshima K
AD  - Department of Pathology, Tokyo Women's Medical University Yachiyo Medical Center, 
      Yachiyo, Japan.
FAU - McGregor, Stephanie M
AU  - McGregor SM
AD  - Department of Pathology, University of Chicago Hospitals, Chicago, Illinois.
FAU - Wu, Di
AU  - Wu D
AD  - Department of Pathology, Tokyo Women's Medical University Yachiyo Medical Center, 
      Yachiyo, Japan.
FAU - Husain, Aliya N
AU  - Husain AN
AD  - Department of Pathology, University of Chicago Hospitals, Chicago, Illinois.
FAU - Marchevsky, Alberto M
AU  - Marchevsky AM
AD  - Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los 
      Angeles, California.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20160428
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
RN  - 0 (BAP1 protein, human)
RN  - 0 (CDKN2A protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Ascitic Fluid/metabolism/*pathology
MH  - Cyclin-Dependent Kinase Inhibitor p16
MH  - Epithelial Cells/metabolism/pathology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mesothelioma/metabolism/*pathology
MH  - Middle Aged
MH  - Neoplasm Proteins/genetics/*metabolism
MH  - Pleural Effusion, Malignant/metabolism/*pathology
MH  - Tumor Suppressor Proteins/genetics/*metabolism
MH  - Ubiquitin Thiolesterase/genetics/*metabolism
OTO - NOTNLM
OT  - BAP1
OT  - CDKN2A (p16) FISH
OT  - effusion
OT  - malignant mesothelioma
EDAT- 2016/04/29 06:00
MHDA- 2017/01/19 06:00
CRDT- 2016/04/29 06:00
PHST- 2016/01/07 00:00 [received]
PHST- 2016/03/24 00:00 [revised]
PHST- 2016/04/04 00:00 [accepted]
PHST- 2016/04/29 06:00 [entrez]
PHST- 2016/04/29 06:00 [pubmed]
PHST- 2017/01/19 06:00 [medline]
AID - 10.1002/dc.23491 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2016 Jul;44(7):599-606. doi: 10.1002/dc.23491. Epub 2016 Apr 
      28.