PMID- 27128446
OWN - NLM
STAT- MEDLINE
DCOM- 20170508
LR  - 20220408
IS  - 1553-7404 (Electronic)
IS  - 1553-7390 (Print)
IS  - 1553-7390 (Linking)
VI  - 12
IP  - 4
DP  - 2016 Apr
TI  - An Arabidopsis SUMO E3 Ligase, SIZ1, Negatively Regulates Photomorphogenesis by 
      Promoting COP1 Activity.
PG  - e1006016
LID - 10.1371/journal.pgen.1006016 [doi]
LID - e1006016
AB  - COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1), a ubiquitin E3 ligase, is a central 
      negative regulator of photomorphogenesis. However, how COP1 activity is regulated 
      by post-translational modifications remains largely unknown. Here we show that 
      SUMO (small ubiquitin-like modifier) modification enhances COP1 activity. 
      Loss-of-function siz1 mutant seedlings exhibit a weak constitutive 
      photomorphogenic phenotype. SIZ1 physically interacts with COP1 and mediates the 
      sumoylation of COP1. A K193R substitution in COP1 blocks its SUMO modification 
      and reduces COP1 activity in vitro and in planta. Consistently, COP1 activity is 
      reduced in siz1 and the level of HY5, a COP1 target protein, is increased in 
      siz1. Sumoylated COP1 may exhibits higher transubiquitination activity than does 
      non-sumoylated COP1, but SIZ1-mediated SUMO modification does not affect COP1 
      dimerization, COP1-HY5 interaction, and nuclear accumulation of COP1. 
      Interestingly, prolonged light exposure reduces the sumoylation level of COP1, 
      and COP1 mediates the ubiquitination and degradation of SIZ1. These regulatory 
      mechanisms may maintain the homeostasis of COP1 activity, ensuing proper 
      photomorphogenic development in changing light environment. Our genetic and 
      biochemical studies identify a function for SIZ1 in photomorphogenesis and reveal 
      a novel SUMO-regulated ubiquitin ligase, COP1, in plants.
FAU - Lin, Xiao-Li
AU  - Lin XL
AD  - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese 
      Academy of Sciences, Beijing, China.
FAU - Niu, De
AU  - Niu D
AD  - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese 
      Academy of Sciences, Beijing, China.
FAU - Hu, Zi-Liang
AU  - Hu ZL
AD  - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese 
      Academy of Sciences, Beijing, China.
FAU - Kim, Dae Heon
AU  - Kim DH
AD  - Department of Biology, Sunchon National University, Sunchon, Republic of Korea.
FAU - Jin, Yin Hua
AU  - Jin YH
AD  - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese 
      Academy of Sciences, Beijing, China.
FAU - Cai, Bin
AU  - Cai B
AD  - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese 
      Academy of Sciences, Beijing, China.
FAU - Liu, Peng
AU  - Liu P
AD  - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese 
      Academy of Sciences, Beijing, China.
FAU - Miura, Kenji
AU  - Miura K
AD  - Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 
      Japan.
FAU - Yun, Dae-Jin
AU  - Yun DJ
AD  - Division of Applied Life Science (BK21Plus), PMBBRC & IALS, Gyeongsang National 
      University, Jinju, Republic of Korea.
FAU - Kim, Woe-Yeon
AU  - Kim WY
AD  - Division of Applied Life Science (BK21Plus), PMBBRC & IALS, Gyeongsang National 
      University, Jinju, Republic of Korea.
FAU - Lin, Rongcheng
AU  - Lin R
AD  - Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 
      Beijing, China.
FAU - Jin, Jing Bo
AU  - Jin JB
AUID- ORCID: 0000-0001-5847-5138
AD  - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese 
      Academy of Sciences, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20160429
PL  - United States
TA  - PLoS Genet
JT  - PLoS genetics
JID - 101239074
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Basic-Leucine Zipper Transcription Factors)
RN  - 0 (HY5 protein, Arabidopsis)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Small Ubiquitin-Related Modifier Proteins)
RN  - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 6.- (Ligases)
RN  - EC 6.3.2.- (SIZ1 protein, Arabidopsis)
SB  - IM
MH  - Amino Acid Substitution/genetics
MH  - Arabidopsis/*genetics/growth & development
MH  - Arabidopsis Proteins/*genetics/metabolism
MH  - Basic-Leucine Zipper Transcription Factors/genetics/metabolism
MH  - Gene Expression Regulation, Plant
MH  - Ligases/*genetics/metabolism
MH  - Light
MH  - Mutation
MH  - Nuclear Proteins/genetics/metabolism
MH  - Plant Development/*genetics
MH  - Proteolysis
MH  - Seedlings/genetics/growth & development
MH  - Small Ubiquitin-Related Modifier Proteins/genetics/metabolism
MH  - Sumoylation/genetics
MH  - Ubiquitin-Protein Ligases/*genetics/metabolism
MH  - Ubiquitination/genetics
PMC - PMC4851335
COIS- The authors have declared that no competing interests exist.
EDAT- 2016/04/30 06:00
MHDA- 2017/05/10 06:00
PMCR- 2016/04/29
CRDT- 2016/04/30 06:00
PHST- 2016/01/03 00:00 [received]
PHST- 2016/04/07 00:00 [accepted]
PHST- 2016/04/30 06:00 [entrez]
PHST- 2016/04/30 06:00 [pubmed]
PHST- 2017/05/10 06:00 [medline]
PHST- 2016/04/29 00:00 [pmc-release]
AID - PGENETICS-D-16-00004 [pii]
AID - 10.1371/journal.pgen.1006016 [doi]
PST - epublish
SO  - PLoS Genet. 2016 Apr 29;12(4):e1006016. doi: 10.1371/journal.pgen.1006016. 
      eCollection 2016 Apr.