PMID- 27128744
OWN - NLM
STAT- MEDLINE
DCOM- 20180820
LR  - 20180820
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 88
IP  - 11
DP  - 2016 Jun 7
TI  - Assessment of Binding Affinity between Drugs and Human Serum Albumin Using 
      Nanoporous Anodic Alumina Photonic Crystals.
PG  - 5971-80
LID - 10.1021/acs.analchem.6b00993 [doi]
AB  - In this study, we report an innovative approach aiming to assess the binding 
      affinity between drug molecules and human serum albumin by combining nanoporous 
      anodic alumina rugate filters (NAA-RFs) modified with human serum albumin (HSA) 
      and reflectometric interference spectroscopy (RIfS). NAA-RFs are photonic crystal 
      structures produced by sinusoidal pulse anodization of aluminum that present two 
      characteristic optical parameters, the characteristic reflection peak (lambdaPeak), 
      and the effective optical thickness of the film (OTeff), which can be readily 
      used as sensing parameters. A design of experiments strategy and an ANOVA 
      analysis are used to establish the effect of the anodization parameters (i.e., 
      anodization period and anodization offset) on the sensitivity of HSA-modified 
      NAA-RFs toward indomethacin, a model drug. To this end, two sensing parameters 
      are used, that is, shifts in the characteristic reflection peak (DeltalambdaPeak) and 
      changes in the effective optical thickness of the film (DeltaOTeff). Subsequently, 
      optimized NAA-RFs are used as sensing platforms to determine the binding affinity 
      between a set of drugs (i.e., indomethacin, coumarin, sulfadymethoxine, warfarin, 
      and salicylic acid) and HSA molecules. Our results verify that the combination of 
      HSA-modified NAA-RFs with RIfS can be used as a portable, low-cost, and simple 
      system for establishing the binding affinity between drugs and plasma proteins, 
      which is a critical factor to develop efficient medicines for treating a broad 
      range of diseases and medical conditions.
FAU - Nemati, Mahdieh
AU  - Nemati M
AD  - School of Chemical Engineering, The University of Adelaide , Engineering North 
      Building, 5005 Adelaide, Australia.
FAU - Santos, Abel
AU  - Santos A
AD  - School of Chemical Engineering, The University of Adelaide , Engineering North 
      Building, 5005 Adelaide, Australia.
FAU - Law, Cheryl Suwen
AU  - Law CS
AD  - School of Chemical Engineering, The University of Adelaide , Engineering North 
      Building, 5005 Adelaide, Australia.
FAU - Losic, Dusan
AU  - Losic D
AD  - School of Chemical Engineering, The University of Adelaide , Engineering North 
      Building, 5005 Adelaide, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160510
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Coumarins)
RN  - 30CPC5LDEX (Sulfadimethoxine)
RN  - 5Q7ZVV76EI (Warfarin)
RN  - A4VZ22K1WT (coumarin)
RN  - LMI26O6933 (Aluminum Oxide)
RN  - O414PZ4LPZ (Salicylic Acid)
RN  - XXE1CET956 (Indomethacin)
RN  - ZIF514RVZR (Serum Albumin, Human)
SB  - IM
MH  - Aluminum Oxide/chemistry
MH  - Biosensing Techniques
MH  - Coumarins/*chemistry
MH  - Crystallization
MH  - Electrodes
MH  - Humans
MH  - Indomethacin/*chemistry
MH  - Nanopores
MH  - Optical Phenomena
MH  - Photons
MH  - Salicylic Acid/*chemistry
MH  - Serum Albumin, Human/*chemistry
MH  - Sulfadimethoxine/*chemistry
MH  - Warfarin/*chemistry
EDAT- 2016/04/30 06:00
MHDA- 2018/08/21 06:00
CRDT- 2016/04/30 06:00
PHST- 2016/04/30 06:00 [entrez]
PHST- 2016/04/30 06:00 [pubmed]
PHST- 2018/08/21 06:00 [medline]
AID - 10.1021/acs.analchem.6b00993 [doi]
PST - ppublish
SO  - Anal Chem. 2016 Jun 7;88(11):5971-80. doi: 10.1021/acs.analchem.6b00993. Epub 
      2016 May 10.