PMID- 27128803
OWN - NLM
STAT- MEDLINE
DCOM- 20170414
LR  - 20231213
IS  - 1470-8736 (Electronic)
IS  - 0143-5221 (Print)
IS  - 0143-5221 (Linking)
VI  - 130
IP  - 10
DP  - 2016 May 1
TI  - Influenza A virus infection and cigarette smoke impair bronchodilator 
      responsiveness to beta-adrenoceptor agonists in mouse lung.
PG  - 829-37
LID - 10.1042/CS20160093 [doi]
AB  - beta2-adrenoceptor agonists are the mainstay therapy for patients with asthma but 
      their effectiveness in cigarette smoke (CS)-induced lung disease such as chronic 
      obstructive pulmonary disease (COPD) is limited. In addition, bronchodilator 
      efficacy of beta2-adrenoceptor agonists is decreased during acute exacerbations of 
      COPD (AECOPD), caused by respiratory viruses including influenza A. Therefore, 
      the aim of the present study was to assess the effects of the beta2-adrenoceptor 
      agonist salbutamol (SALB) on small airway reactivity using mouse precision cut 
      lung slices (PCLS) prepared from CS-exposed mice and from CS-exposed mice treated 
      with influenza A virus (Mem71, H3N1). CS exposure alone reduced SALB potency and 
      efficacy associated with decreased beta2-adrenoceptor mRNA expression, and increased 
      tumour necrosis factor alpha (TNFalpha) and interleukin-1beta (IL-1beta) expression. This 
      impaired relaxation was restored by day 12 in the absence of further CS exposure. 
      In PCLS prepared after Mem71 infection alone, responses to SALB were transient 
      and were not well maintained. CS exposure prior to Mem71 infection almost 
      completely abolished relaxation, although beta2-adrenoceptor and TNFalpha and IL-1beta 
      expression were unaltered. The present study has shown decreased sensitivity to 
      SALB after CS or a combination of CS and Mem71 occurs by different mechanisms. In 
      addition, the PCLS technique and our models of CS and influenza infection provide 
      a novel setting for assessment of alternative bronchodilators.
CI  - (c) 2016 The Author(s).
FAU - Donovan, Chantal
AU  - Donovan C
AD  - Biomedicine Discovery Institute, Department of Pharmacology, Monash University, 
      Clayton, Victoria 3800, Australia Lung Health Research Centre, Department of 
      Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria 3010, 
      Australia.
FAU - Seow, Huei Jiunn
AU  - Seow HJ
AD  - Lung Health Research Centre, Department of Pharmacology and Therapeutics, 
      University of Melbourne, Parkville, Victoria 3010, Australia School of Health and 
      Biomedical Sciences, RMIT University, Bundoora, Victoria 3083, Australia.
FAU - Bourke, Jane E
AU  - Bourke JE
AD  - Biomedicine Discovery Institute, Department of Pharmacology, Monash University, 
      Clayton, Victoria 3800, Australia Lung Health Research Centre, Department of 
      Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria 3010, 
      Australia.
FAU - Vlahos, Ross
AU  - Vlahos R
AD  - Lung Health Research Centre, Department of Pharmacology and Therapeutics, 
      University of Melbourne, Parkville, Victoria 3010, Australia School of Health and 
      Biomedical Sciences, RMIT University, Bundoora, Victoria 3083, Australia 
      ross.vlahos@rmit.edu.au.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160410
PL  - England
TA  - Clin Sci (Lond)
JT  - Clinical science (London, England : 1979)
JID - 7905731
RN  - 0 (Adrenergic beta-Agonists)
RN  - 0 (Bronchodilator Agents)
RN  - 0 (Receptors, Adrenergic)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
CIN - Clin Sci (Lond). 2016 May 1;130(10):839-41. PMID: 27128804
MH  - Adrenergic beta-Agonists/*pharmacology
MH  - Animals
MH  - Bronchodilator Agents/*therapeutic use
MH  - *Influenza A virus
MH  - Lung/metabolism/*virology
MH  - Male
MH  - Mice
MH  - Pulmonary Disease, Chronic Obstructive/drug therapy/metabolism
MH  - Receptors, Adrenergic/metabolism
MH  - Smoking/*adverse effects
MH  - Nicotiana/adverse effects
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC5233570
OTO - NOTNLM
OT  - acute exacerbations of COPD (AECOPD)
OT  - chronic obstructive pulmonary disease (COPD)
OT  - influenza
OT  - respiratory virus
OT  - small airways
OT  - beta-adrenoceptor
EDAT- 2016/04/30 06:00
MHDA- 2017/04/15 06:00
PMCR- 2016/04/10
CRDT- 2016/04/30 06:00
PHST- 2015/11/23 00:00 [received]
PHST- 2016/02/23 00:00 [accepted]
PHST- 2016/04/30 06:00 [entrez]
PHST- 2016/04/30 06:00 [pubmed]
PHST- 2017/04/15 06:00 [medline]
PHST- 2016/04/10 00:00 [pmc-release]
AID - CS20160093 [pii]
AID - 10.1042/CS20160093 [doi]
PST - ppublish
SO  - Clin Sci (Lond). 2016 May 1;130(10):829-37. doi: 10.1042/CS20160093. Epub 2016 
      Apr 10.