PMID- 27129193
OWN - NLM
STAT- MEDLINE
DCOM- 20170621
LR  - 20231213
IS  - 1470-8736 (Electronic)
IS  - 0143-5221 (Linking)
VI  - 130
IP  - 14
DP  - 2016 Jul 1
TI  - Tobacco toxins deposited on surfaces (third hand smoke) impair wound healing.
PG  - 1269-84
LID - 10.1042/CS20160236 [doi]
AB  - Third hand smoke (THS) is the accumulation of second hand smoke (SHS) toxins on 
      surfaces in homes, cars, clothing and hair of smokers. It is known that 88M US 
      nonsmokers >/=3 years old living in homes of smokers are exposed to THS toxicants 
      and show blood cotinine levels of >/=0.05 ng/ml, indicating that the toxins are 
      circulating in their circulatory systems. The goal of the present study is to 
      investigate the mechanisms by which THS causes impaired wound healing. We show 
      that mice living under conditions that mimic THS exposure in humans display 
      delayed wound closure, impaired collagen deposition, altered inflammatory 
      response, decreased angiogenesis, microvessels with fibrin cuffs and a highly 
      proteolytic wound environment. Moreover, THS-exposed mouse wounds have high 
      levels of oxidative stress and significantly lower levels of antioxidant activity 
      leading to molecular damage, including protein nitration, lipid peroxidation and 
      DNA damage that contribute to tissue dysfunction. Furthermore, we show that 
      elastase is elevated, suggesting that elastin is degraded and the plasticity of 
      the wound tissue is decreased. Taken together, our results lead us to conclude 
      that THS toxicants delay and impair wound healing by disrupting the sequential 
      processes that lead to normal healing. In addition, the lack of elastin results 
      in loss of wound plasticity, which may be responsible for reopening of wounds.
CI  - (c) 2016 The Author(s). published by Portland Press Limited on behalf of the 
      Biochemical Society.
FAU - Dhall, Sandeep
AU  - Dhall S
AD  - Department of Cell Biology and Neuroscience, University of California at 
      Riverside, Riverside, CA 92521, U.S.A.
FAU - Alamat, Raquelle
AU  - Alamat R
AD  - Department of Cell Biology and Neuroscience, University of California at 
      Riverside, Riverside, CA 92521, U.S.A.
FAU - Castro, Anthony
AU  - Castro A
AD  - Department of Cell Biology and Neuroscience, University of California at 
      Riverside, Riverside, CA 92521, U.S.A.
FAU - Sarker, Altaf H
AU  - Sarker AH
AD  - Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, 
      Berkeley, CA 94720, U.S.A.
FAU - Mao, Jian-Hua
AU  - Mao JH
AD  - Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, 
      Berkeley, CA 94720, U.S.A.
FAU - Chan, Alex
AU  - Chan A
AD  - Department of Cell Biology and Neuroscience, University of California at 
      Riverside, Riverside, CA 92521, U.S.A.
FAU - Hang, Bo
AU  - Hang B
AD  - Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, 
      Berkeley, CA 94720, U.S.A.
FAU - Martins-Green, Manuela
AU  - Martins-Green M
AD  - Department of Cell Biology and Neuroscience, University of California at 
      Riverside, Riverside, CA 92521, U.S.A. manuela.martins@ucr.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160428
PL  - England
TA  - Clin Sci (Lond)
JT  - Clinical science (London, England : 1979)
JID - 7905731
RN  - 0 (Chemokines)
RN  - 0 (Timp1 protein, mouse)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 0 (Tobacco Smoke Pollution)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Capillary Permeability
MH  - Chemokines/biosynthesis
MH  - Collagen/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neovascularization, Physiologic
MH  - Oxidative Stress
MH  - Tissue Inhibitor of Metalloproteinase-1/analysis
MH  - Nicotiana/*adverse effects
MH  - Tobacco Smoke Pollution/*adverse effects
MH  - *Wound Healing
OTO - NOTNLM
OT  - DNA damage
OT  - angiogenesis
OT  - cytokine
OT  - inflammation
OT  - reactive oxygen species and antioxidants
OT  - toxicants
EDAT- 2016/04/30 06:00
MHDA- 2017/06/22 06:00
CRDT- 2016/04/30 06:00
PHST- 2016/03/29 00:00 [received]
PHST- 2016/04/28 00:00 [accepted]
PHST- 2016/04/30 06:00 [entrez]
PHST- 2016/04/30 06:00 [pubmed]
PHST- 2017/06/22 06:00 [medline]
AID - CS20160236 [pii]
AID - 10.1042/CS20160236 [doi]
PST - ppublish
SO  - Clin Sci (Lond). 2016 Jul 1;130(14):1269-84. doi: 10.1042/CS20160236. Epub 2016 
      Apr 28.