PMID- 27129299
OWN - NLM
STAT- MEDLINE
DCOM- 20171219
LR  - 20211204
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 36
IP  - 3
DP  - 2016 Jul
TI  - Leucine alleviates dexamethasone-induced suppression of muscle protein synthesis 
      via synergy involvement of mTOR and AMPK pathways.
LID - 10.1042/BSR20160096 [doi]
LID - e00346
AB  - Glucocorticoids (GCs) are negative muscle protein regulators that contribute to 
      the whole-body catabolic state during stress. Mammalian target of rapamycin 
      (mTOR)-signalling pathway, which acts as a central regulator of protein 
      metabolism, can be activated by branched-chain amino acids (BCAA). In the present 
      study, the effect of leucine on the suppression of protein synthesis induced by 
      GCs and the pathway involved were investigated. In vitro experiments were 
      conducted using cultured C2C12 myoblasts to study the effect of GCs on protein 
      synthesis, and the involvement of mTOR pathway was investigated as well. After 
      exposure to dexamethasone (DEX, 100 mumol/l) for 24 h, protein synthesis in muscle 
      cells was significantly suppressed (P<0.05), the phosphorylations of mTOR, 
      ribosomal protein S6 protein kinase 1 (p70s6k1) and eukaryotic initiation factor 
      4E binding protein 1 (4EBP1) were significantly reduced (P<0.05). Leucine 
      supplementation (5 mmol/l, 10 mmol/l and 15 mmol/l) for 1 h alleviated the 
      suppression of protein synthesis induced by DEX (P<0.05) and was accompanied with 
      the increased phosphorylation of mTOR and decreased phosphorylation of AMPK 
      (P<0.05). Branched-chain amino transferase 2 (BCAT2) mRNA level was not 
      influenced by DEX (P>0.05) but was increased by leucine supplementation at a dose 
      of 5 mmol/l (P<0.05).
CI  - (c) 2016 The Author(s).
FAU - Wang, Xiao J
AU  - Wang XJ
AD  - Department of Animal Science, Shandong Agricultural University, Taian, Shandong 
      271018, P.R. China.
FAU - Yang, Xin
AU  - Yang X
AD  - Department of Animal Science, Shandong Agricultural University, Taian, Shandong 
      271018, P.R. China.
FAU - Wang, Ru X
AU  - Wang RX
AD  - Department of Animal Science, Shandong Agricultural University, Taian, Shandong 
      271018, P.R. China.
FAU - Jiao, Hong C
AU  - Jiao HC
AD  - Department of Animal Science, Shandong Agricultural University, Taian, Shandong 
      271018, P.R. China.
FAU - Zhao, Jing P
AU  - Zhao JP
AD  - Department of Animal Science, Shandong Agricultural University, Taian, Shandong 
      271018, P.R. China.
FAU - Song, Zhi G
AU  - Song ZG
AD  - Department of Animal Science, Shandong Agricultural University, Taian, Shandong 
      271018, P.R. China.
FAU - Lin, Hai
AU  - Lin H
AD  - Department of Animal Science, Shandong Agricultural University, Taian, Shandong 
      271018, P.R. China hailin@sdau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160617
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (Glucocorticoids)
RN  - 0 (Muscle Proteins)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - AMP-Activated Protein Kinases/*metabolism
MH  - Animals
MH  - Cell Line
MH  - Dexamethasone/*pharmacology
MH  - Glucocorticoids/*pharmacology
MH  - Leucine/*pharmacology
MH  - Mice
MH  - Muscle Proteins/*metabolism
MH  - Myoblasts/drug effects/metabolism
MH  - Phosphorylation/drug effects
MH  - Protein Biosynthesis/*drug effects
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/*metabolism
PMC - PMC5293580
OTO - NOTNLM
OT  - AMPK
OT  - glucocorticoid
OT  - leucine
OT  - mTOR
OT  - muscle cell
OT  - protein synthesis
EDAT- 2016/04/30 06:00
MHDA- 2017/12/20 06:00
PMCR- 2016/06/01
CRDT- 2016/04/30 06:00
PHST- 2016/04/02 00:00 [received]
PHST- 2016/04/28 00:00 [accepted]
PHST- 2016/04/30 06:00 [entrez]
PHST- 2016/04/30 06:00 [pubmed]
PHST- 2017/12/20 06:00 [medline]
PHST- 2016/06/01 00:00 [pmc-release]
AID - BSR20160096 [pii]
AID - e00346 [pii]
AID - 10.1042/BSR20160096 [doi]
PST - epublish
SO  - Biosci Rep. 2016 Jun 17;36(3):e00346. doi: 10.1042/BSR20160096. Print 2016 Jul.