PMID- 27131287
OWN - NLM
STAT- MEDLINE
DCOM- 20170208
LR  - 20220409
IS  - 1872-7077 (Electronic)
IS  - 1382-6689 (Linking)
VI  - 44
DP  - 2016 Jun
TI  - Morin downregulates nitric oxide and prostaglandin E2 production in 
      LPS-stimulated BV2 microglial cells by suppressing NF-kappaB activity and activating 
      HO-1 induction.
PG  - 62-8
LID - S1382-6689(16)30087-4 [pii]
LID - 10.1016/j.etap.2016.04.010 [doi]
AB  - Morin possesses anti-inflammatory activity against septic shock and allergic 
      responses, and prevents acute liver damage. However, the biological mechanism of 
      action of morin in neuroinflammation remains largely unknown. Therefore, the 
      present study investigated whether morin has the ability to attenuate expression 
      of proinflammatory mediators such as nitric oxide (NO) and prostaglandin E2 
      (PGE2) in lipopolysaccharide (LPS)-stimulated BV2 microglial cells. Morin 
      inhibited the expression of LPS-induced proinflammatory mediators such as NO and 
      PGE2, without any cytotoxic effects. Furthermore, LPS-induced inducible NO 
      synthase (iNOS) and cyclooxygenase-2 (COX-2) were inhibited both at the mRNA and 
      protein levels in response to morin. Morin also attenuated LPS-induced 
      DNA-binding activity of nuclear transcription factor-kappaB (NF-kappaB) and its promoter 
      activity. Pyrrolidine dithiocarbamate (PDTC), a specific NF-kappaB inhibitor, 
      downregulated the expression of LPS-induced iNOS and COX-2, which suggests that 
      morin-mediated NF-kappaB inhibition is the main signaling pathway responsible for the 
      inhibition of iNOS and COX-2 expression. Additionally, morin increased induction 
      of heme oxygenase-1 (HO-1) activity, leading to the suppression of NO and PGE2 
      production. Our results indicate that morin downregulates the expression of 
      proinflammatory genes, such as iNOS and COX-2, involved in the synthesis of NO 
      and PGE2 in LPS-stimulated BV2 microglial cells by suppressing NF-kappaB activity and 
      activation of HO-1. Taken together, the findings of the present study suggest 
      that morin may have potential as a therapeutic for the prevention of 
      neuroinflammation.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Dilshara, Matharage Gayani
AU  - Dilshara MG
AD  - Department of Marine Life Sciences, Jeju National University, Jeju 63243, 
      Republic of Korea.
FAU - Jayasooriya, Rajapaksha Gedara Prasad Tharanga
AU  - Jayasooriya RG
AD  - Department of Marine Life Sciences, Jeju National University, Jeju 63243, 
      Republic of Korea.
FAU - Lee, Seungheon
AU  - Lee S
AD  - Department of Marine Life Sciences, Jeju National University, Jeju 63243, 
      Republic of Korea.
FAU - Choi, Yung Hyun
AU  - Choi YH
AD  - Department of Biochemistry, College of Oriental Medicine, Dong-Eui University, 
      Busan47340, Republic of Korea.
FAU - Kim, Gi-Young
AU  - Kim GY
AD  - Department of Marine Life Sciences, Jeju National University, Jeju 63243, 
      Republic of Korea. Electronic address: immunkim@jejunu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20160422
PL  - Netherlands
TA  - Environ Toxicol Pharmacol
JT  - Environmental toxicology and pharmacology
JID - 9612020
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Flavonoids)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Proteins)
RN  - 0 (NF-kappa B)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 8NFQ3F76WR (morin)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, mouse)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.14.14.18 (Hmox1 protein, mouse)
RN  - EC 1.14.99.- (Ptgs2 protein, mouse)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Cell Line
MH  - Cyclooxygenase 2/genetics/metabolism
MH  - Dinoprostone/*metabolism
MH  - Down-Regulation
MH  - Flavonoids/*pharmacology
MH  - Heme Oxygenase-1/genetics/*metabolism
MH  - Lipopolysaccharides
MH  - Membrane Proteins/genetics/*metabolism
MH  - Mice
MH  - Microglia/*drug effects/metabolism
MH  - NF-kappa B/*metabolism
MH  - Nitric Oxide/*metabolism
MH  - Nitric Oxide Synthase Type II/genetics/metabolism
OTO - NOTNLM
OT  - Heme oxygenase-1
OT  - Morin
OT  - Nitric oxide
OT  - Nuclear factor-kappaB
OT  - Prostaglandin E(2)
EDAT- 2016/05/01 06:00
MHDA- 2017/02/09 06:00
CRDT- 2016/05/01 06:00
PHST- 2016/02/05 00:00 [received]
PHST- 2016/04/19 00:00 [revised]
PHST- 2016/04/20 00:00 [accepted]
PHST- 2016/05/01 06:00 [entrez]
PHST- 2016/05/01 06:00 [pubmed]
PHST- 2017/02/09 06:00 [medline]
AID - S1382-6689(16)30087-4 [pii]
AID - 10.1016/j.etap.2016.04.010 [doi]
PST - ppublish
SO  - Environ Toxicol Pharmacol. 2016 Jun;44:62-8. doi: 10.1016/j.etap.2016.04.010. 
      Epub 2016 Apr 22.