PMID- 27135604
OWN - NLM
STAT- MEDLINE
DCOM- 20170531
LR  - 20220311
IS  - 1529-2916 (Electronic)
IS  - 1529-2908 (Print)
IS  - 1529-2908 (Linking)
VI  - 17
IP  - 7
DP  - 2016 Jul
TI  - Expression profiling of constitutive mast cells reveals a unique identity within 
      the immune system.
PG  - 878-87
LID - 10.1038/ni.3445 [doi]
AB  - Mast cells are evolutionarily ancient sentinel cells. Like basophils, mast cells 
      express the high-affinity receptor for immunoglobulin E (IgE) and have been 
      linked to host defense and diverse immune-system-mediated diseases. To better 
      characterize the function of these cells, we assessed the transcriptional 
      profiles of mast cells isolated from peripheral connective tissues and basophils 
      isolated from spleen and blood. We found that mast cells were transcriptionally 
      distinct, clustering independently from all other profiled cells, and that mast 
      cells demonstrated considerably greater heterogeneity across tissues than 
      previously appreciated. We observed minimal homology between mast cells and 
      basophils, which shared more overlap with other circulating granulocytes than 
      with mast cells. The derivation of mast-cell and basophil transcriptional 
      signatures underscores their differential capacities to detect environmental 
      signals and influence the inflammatory milieu.
FAU - Dwyer, Daniel F
AU  - Dwyer DF
AD  - Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, 
      Boston, Massachusetts, USA.
AD  - Harvard Medical School, Boston, Massachusetts, USA.
FAU - Barrett, Nora A
AU  - Barrett NA
AD  - Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, 
      Boston, Massachusetts, USA.
AD  - Harvard Medical School, Boston, Massachusetts, USA.
FAU - Austen, K Frank
AU  - Austen KF
AD  - Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, 
      Boston, Massachusetts, USA.
AD  - Harvard Medical School, Boston, Massachusetts, USA.
CN  - Immunological Genome Project Consortium
LA  - eng
GR  - R01 HL120952/HL/NHLBI NIH HHS/United States
GR  - R24 AI072073/AI/NIAID NIH HHS/United States
GR  - T32 AI007306/AI/NIAID NIH HHS/United States
GR  - U19 AI095219/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
DEP - 20160502
PL  - United States
TA  - Nat Immunol
JT  - Nature immunology
JID - 100941354
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Basophils/*physiology
MH  - Blood Cells/*physiology
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Connective Tissue Cells/*physiology
MH  - Flow Cytometry
MH  - Gene Expression Profiling
MH  - Immunoglobulin E/metabolism
MH  - Male
MH  - Mast Cells/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Spleen/*cytology
MH  - Tissue Array Analysis
PMC - PMC5045264
MID - NIHMS772407
COIS- The authors have no competing financial interest
FIR - Dwyer, Daniel F
IR  - Dwyer DF
FIR - Barrett, Nora A
IR  - Barrett NA
FIR - Austen, K Frank
IR  - Austen KF
FIR - Kim, Edy Y
IR  - Kim EY
FIR - Brenner, Michael B
IR  - Brenner MB
FIR - Shaw, Laura
IR  - Shaw L
FIR - Yu, Bingfei
IR  - Yu B
FIR - Goldrath, Ananda
IR  - Goldrath A
FIR - Mostafavi, Sara
IR  - Mostafavi S
FIR - Regev, Aviv
IR  - Regev A
FIR - Rhoades, Andrew
IR  - Rhoades A
FIR - Moodley, Devapregasan
IR  - Moodley D
FIR - Yoshida, Hideyuki
IR  - Yoshida H
FIR - Mathis, Diane
IR  - Mathis D
FIR - Benoist, Christophe
IR  - Benoist C
FIR - Nabekura, Tsukasa
IR  - Nabekura T
FIR - Lam, Viola
IR  - Lam V
FIR - Lanier, Lewis L
IR  - Lanier LL
FIR - Brown, Brian
IR  - Brown B
FIR - Merad, Miriam
IR  - Merad M
FIR - Cremasco, Viviana
IR  - Cremasco V
FIR - Turley, Shannon
IR  - Turley S
FIR - Monach, Paul
IR  - Monach P
FIR - Dustin, Michael L
IR  - Dustin ML
FIR - Li, Yuesheng
IR  - Li Y
FIR - Shinton, Susan A
IR  - Shinton SA
FIR - Hardy, Richard R
IR  - Hardy RR
FIR - Shay, Tal
IR  - Shay T
FIR - Qi, Yilin
IR  - Qi Y
FIR - Sylvia, Katelyn
IR  - Sylvia K
FIR - Kang, Joonsoo
IR  - Kang J
FIR - Fairfax, Keke
IR  - Fairfax K
FIR - Randolph, Gwendalyn J
IR  - Randolph GJ
FIR - Robinette, Michelle L
IR  - Robinette ML
FIR - Fuchs, Anja
IR  - Fuchs A
FIR - Colonna, Marco
IR  - Colonna M
EDAT- 2016/05/03 06:00
MHDA- 2017/06/01 06:00
PMCR- 2016/11/02
CRDT- 2016/05/03 06:00
PHST- 2015/08/18 00:00 [received]
PHST- 2016/03/23 00:00 [accepted]
PHST- 2016/05/03 06:00 [entrez]
PHST- 2016/05/03 06:00 [pubmed]
PHST- 2017/06/01 06:00 [medline]
PHST- 2016/11/02 00:00 [pmc-release]
AID - ni.3445 [pii]
AID - 10.1038/ni.3445 [doi]
PST - ppublish
SO  - Nat Immunol. 2016 Jul;17(7):878-87. doi: 10.1038/ni.3445. Epub 2016 May 2.