PMID- 27135915
OWN - NLM
STAT- MEDLINE
DCOM- 20170505
LR  - 20201209
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Print)
IS  - 0019-2805 (Linking)
VI  - 148
IP  - 4
DP  - 2016 Aug
TI  - Distinct expression of interferon-induced protein with tetratricopeptide repeats 
      (IFIT) 1/2/3 and other antiviral genes between subsets of dendritic cells induced 
      by dengue virus 2 infection.
PG  - 363-76
LID - 10.1111/imm.12615 [doi]
AB  - Dengue virus (DENV) infection is an emerging public health hazard threatening 
      inhabitants of the tropics and sub-tropics. Dendritic cells (DCs) are one of the 
      major targets of DENV and the initiators of the innate immune response against 
      the virus. However, current in vitro research on the DENV-DC interaction is 
      hampered by the low availability of ex vivo DCs and donor variation. In the 
      current study, we attempted to develop a novel in vitro DC model using immature 
      DCs derived from the myeloid leukaemia cell line MUTZ-3 (IMDCs) to investigate 
      the DENV-DC interaction. The IMDCs morphologically and phenotypically resembled 
      human immature monocyte-derived dendritic cells (IMMoDCs). However, the 
      permissiveness of IMDCs to DENV2 was lower than that of IMMoDCs. RT-PCR arrays 
      showed that a group of type I interferon (IFN) -inducible genes, especially 
      IFIT1, IFITM1, and IFI27, were significantly up-regulated in IMMoDCs but not in 
      IMDCs after DENV2 infection. Further investigation revealed that IFIT genes were 
      spontaneously expressed at both transcriptional and protein levels in the naive 
      IMDCs but not in the naive IMMoDCs. It is possible that the poor permissiveness 
      of IMDCs to DENV2 was a result of the high basal levels of IFIT proteins. We 
      conclude that the IMDC model, although less permissive to DENV2, is a useful 
      platform for studying the suppression mechanism of DENV2 and we expand the 
      knowledge of cellular factors that modulate DENV2 infection in the human body.
CI  - (c) 2016 John Wiley & Sons Ltd.
FAU - Zhang, Jingshu
AU  - Zhang J
AD  - Department of Health Technology and Informatics, The Hong Kong Polytechnic 
      University, Hong Kong, China.
AD  - HKU-Pasteur Research Pole, School of Public Health, The University of Hong Kong, 
      Hong Kong, China.
FAU - Sze, Daniel Man-Yuen
AU  - Sze DM
AD  - Department of Health Technology and Informatics, The Hong Kong Polytechnic 
      University, Hong Kong, China.
AD  - School of Health and Biomedical Sciences, Royal Melbourne Institute of Technology 
      (RMIT) University, Melbourne, VIC, Australia.
FAU - Yung, Benjamin Yat-Ming
AU  - Yung BY
AD  - Department of Health Technology and Informatics, The Hong Kong Polytechnic 
      University, Hong Kong, China.
FAU - Tang, Petrus
AU  - Tang P
AD  - Molecular Regulation and Bioinformatics Laboratory, Department of Public Health 
      and Parasitology, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.
AD  - Graduate Institute of Biomedical Sciences, Chang Gung University, Kwei-San, 
      Tao-Yuan, Taiwan.
FAU - Chen, Wei-June
AU  - Chen WJ
AD  - Graduate Institute of Biomedical Sciences, Chang Gung University, Kwei-San, 
      Tao-Yuan, Taiwan.
AD  - Department of Public Health and Parasitology, College of Medicine, Chang Gung 
      University, Kwei-San, Tao-Yuan, Taiwan.
FAU - Chan, Kwok-Hung
AU  - Chan KH
AD  - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of 
      Hong Kong, Hong Kong, Hong Kong.
FAU - Leung, Polly Hang-Mei
AU  - Leung PH
AD  - Department of Health Technology and Informatics, The Hong Kong Polytechnic 
      University, Hong Kong, China.
LA  - eng
SI  - GENBANK/NM_000395
SI  - GENBANK/NM_000566
SI  - GENBANK/NM_001548
SI  - GENBANK/NM_001549
SI  - GENBANK/NM_001565
SI  - GENBANK/NM_001572
SI  - GENBANK/NM_001992
SI  - GENBANK/NM_002038
SI  - GENBANK/NM_002053
SI  - GENBANK/NM_002198
SI  - GENBANK/NM_002199
SI  - GENBANK/NM_002201
SI  - GENBANK/NM_002463
SI  - GENBANK/NM_002534
SI  - GENBANK/NM_002535
SI  - GENBANK/NM_002744
SI  - GENBANK/NM_002759
SI  - GENBANK/NM_002838
SI  - GENBANK/NM_003641
SI  - GENBANK/NM_003877
SI  - GENBANK/NM_004219
SI  - GENBANK/NM_004688
SI  - GENBANK/NM_005101
SI  - GENBANK/NM_005419
SI  - GENBANK/NM_005531
SI  - GENBANK/NM_005532
SI  - GENBANK/NM_005902
SI  - GENBANK/NM_006084
SI  - GENBANK/NM_006435
SI  - GENBANK/NM_007315
SI  - GENBANK/NM_018191
SI  - GENBANK/NM_022168
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Carrier Proteins)
RN  - 0 (IFI27 protein, human)
RN  - 0 (IFIT1 protein, human)
RN  - 0 (Interferon Type I)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (leu-13 antigen)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Antigens, Differentiation/genetics/*metabolism
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cell Differentiation
MH  - Cell Line
MH  - Dendritic Cells/physiology/*virology
MH  - Dengue/genetics/*immunology
MH  - Dengue Virus/*immunology
MH  - Humans
MH  - Immunity, Innate/genetics
MH  - Interferon Type I/metabolism
MH  - Membrane Proteins/genetics/*metabolism
MH  - Monocytes/physiology/*virology
MH  - RNA-Binding Proteins
MH  - Transcriptome
MH  - Up-Regulation
PMC - PMC4948034
OTO - NOTNLM
OT  - MUTZ-3
OT  - dendritic cells
OT  - dengue virus
EDAT- 2016/05/03 06:00
MHDA- 2017/05/06 06:00
PMCR- 2017/08/01
CRDT- 2016/05/03 06:00
PHST- 2015/07/22 00:00 [received]
PHST- 2016/04/02 00:00 [revised]
PHST- 2016/04/19 00:00 [accepted]
PHST- 2016/05/03 06:00 [entrez]
PHST- 2016/05/03 06:00 [pubmed]
PHST- 2017/05/06 06:00 [medline]
PHST- 2017/08/01 00:00 [pmc-release]
AID - IMM12615 [pii]
AID - 10.1111/imm.12615 [doi]
PST - ppublish
SO  - Immunology. 2016 Aug;148(4):363-76. doi: 10.1111/imm.12615.