PMID- 27140333
OWN - NLM
STAT- MEDLINE
DCOM- 20170303
LR  - 20220410
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 155
DP  - 2016 Jun 15
TI  - Non-canonical role of matrix metalloprotease (MMP) in activation and migration of 
      hepatic stellate cells (HSCs).
PG  - 155-60
LID - S0024-3205(16)30251-X [pii]
LID - 10.1016/j.lfs.2016.04.031 [doi]
AB  - AIMS: Matrix metalloproteinases (MMPs) that degrade extracellular matrix (ECM) 
      and help to resolve the excess matrix are considered to be under-expressed in 
      fibrosis. MMPs are generally anti-fibrotic, however others can have pro-fibrotic 
      functions. Therefore, the aim of this study was to find out the mechanism of 
      pro-fibrotic function of MMPs in hepatic stellate cells' (HSC's) activation and 
      migration. MAIN METHODS: Human MMP Antibody Array from Abcam was used to profile 
      MMPs in macrophages. Gelatin or casein zymography was performed using 10% 
      SDS-polyacrylamide gels (SDS-PAGE) containing gelatin (1mg/ml) or Casein (1mg/ml) 
      as substrate. HSCs migration assay was performed using Boyden chamber as 
      described previously (Guo et al., 2007, McGarrigle et al., 2006, Shan et al., 
      2006 and Yang and Huang, 2005). Real-time PCR with SYBR green was performed using 
      iTaq universal SYBR(R) Green supermix (BIO-RAD) and a 7500 Real-Time PCR System 
      (Applied Biosystems). Collagen, type I, alpha 1 (COL1A1), alpha smooth muscle 
      actin (alpha-SMA) expression was determined by immunoblot analysis. KEY FINDINGS: We 
      first profiled the expression of all MMPs in primary murine bone marrow-derived 
      macrophages (BMDMs) and differentiated THP-1 cells and found that MMP-8, -10, & 
      -13, were significantly overexpressed after 12h of lipopolysaccharide (LPS) 
      treatment. Based on this pattern of expression, we speculated that macrophage 
      MMP-8,-10, &-13 might play a non-canonical role in HSCs activation. Further, we 
      found that exogenous active MMP-8 (Collagenase-2) treated HSC shows markedly 
      increased migration and COL1A1 expression as compared to MMP-10 and MMP-13 
      treated HSCs. Thus, macrophage MMP-8 (Collagenase-2) expression in macrophages 
      emerges as an important moderator of HSC cell migration and invasion. 
      SIGNIFICANCE: These findings suggest that macrophage MMP-8 promotes HSC 
      activation and might have a role in liver disease progression. MMP-8 targeting in 
      the liver may have therapeutic potential in alcoholic liver disease (ALD).
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Baig, Mirza S
AU  - Baig MS
AD  - Center for Biosciences and Biomedical Engineering (BSBE), Indian Institute of 
      Technology Indore (IITI), Indore, MP, India; Division of Gastroenterology and 
      Hepatology, Mayo Clinic, Rochester, MN, USA. Electronic address: msb@iiti.ac.in.
FAU - Yaqoob, Usman
AU  - Yaqoob U
AD  - Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA.
FAU - Cao, Sheng
AU  - Cao S
AD  - Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA.
FAU - Saqib, Uzma
AU  - Saqib U
AD  - Discipline of Chemistry, School of Basic Sciences, Indian Institute of Technology 
      Indore (IITI), Indore, MP, India.
FAU - Shah, Vijay H
AU  - Shah VH
AD  - Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA. 
      Electronic address: Shah.Vijay@mayo.edu.
LA  - eng
PT  - Journal Article
DEP - 20160429
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - *Cell Movement
MH  - Female
MH  - Hepatic Stellate Cells/cytology/*enzymology
MH  - Humans
MH  - Matrix Metalloproteinases/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
OTO - NOTNLM
OT  - Alpha-SMA
OT  - Collagen I
OT  - Fibrosis
OT  - Macrophage
OT  - Matrix metalloproteinase (MMP)
EDAT- 2016/05/04 06:00
MHDA- 2017/03/04 06:00
CRDT- 2016/05/04 06:00
PHST- 2016/03/29 00:00 [received]
PHST- 2016/04/22 00:00 [revised]
PHST- 2016/04/24 00:00 [accepted]
PHST- 2016/05/04 06:00 [entrez]
PHST- 2016/05/04 06:00 [pubmed]
PHST- 2017/03/04 06:00 [medline]
AID - S0024-3205(16)30251-X [pii]
AID - 10.1016/j.lfs.2016.04.031 [doi]
PST - ppublish
SO  - Life Sci. 2016 Jun 15;155:155-60. doi: 10.1016/j.lfs.2016.04.031. Epub 2016 Apr 
      29.