PMID- 2714117
OWN - NLM
STAT- MEDLINE
DCOM- 19890615
LR  - 20151119
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 10
IP  - 3
DP  - 1989 May
TI  - Initial evaluation of a new epithelial antigen (T16) for bivariate flow cytometry 
      of bladder irrigation specimens.
PG  - 339-44
AB  - Bivariate flow cytometry (FCM) was used to study immunofluorescent T16 mouse 
      monoclonal antibody (Mab) binding simultaneously with propidium iodide DNA 
      measurements in bladder irrigation specimens from 30 patients with a history of 
      bladder cancer. Aliquots of the same samples were stained with acridine orange 
      (AO) and examined by conventional FCM. T16 Mab is believed to be specific for 
      epithelial cells in this type of specimen and stained from 13% of the cells in a 
      patient with cystitis to 95% of the cells in a patient with an atypical 
      papilloma. In combination with DNA measurements, this antibody increased the 
      sensitivity of FCM in patients with severe cystitis and relatively small numbers 
      of tumor cells, but the diagnostic specificity may be decreased and the criteria 
      established for interpreting univariate flow cytometry may have to be 
      re-evaluated and modified.
FAU - Bretton, P R
AU  - Bretton PR
AD  - Department of Surgery (Urology Service), Memorial Sloan-Kettering Cancer Center, 
      New York, New York 10021.
FAU - Myc, A
AU  - Myc A
FAU - Cordon-Cardo, C
AU  - Cordon-Cardo C
FAU - DeAngelis, P
AU  - DeAngelis P
FAU - Fair, W R
AU  - Fair WR
FAU - Melamed, M R
AU  - Melamed MR
LA  - eng
GR  - R01-CA-14134/CA/NCI NIH HHS/United States
GR  - UO1-CA-41021/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (DNA, Neoplasm)
RN  - 36015-30-2 (Propidium)
SB  - IM
MH  - *Antibodies, Monoclonal
MH  - Carcinoma/*analysis/genetics
MH  - DNA, Neoplasm/*analysis
MH  - *Flow Cytometry
MH  - Humans
MH  - Propidium
MH  - Urinary Bladder Neoplasms/*analysis/genetics
EDAT- 1989/05/01 00:00
MHDA- 1989/05/01 00:01
CRDT- 1989/05/01 00:00
PHST- 1989/05/01 00:00 [pubmed]
PHST- 1989/05/01 00:01 [medline]
PHST- 1989/05/01 00:00 [entrez]
AID - 10.1002/cyto.990100314 [doi]
PST - ppublish
SO  - Cytometry. 1989 May;10(3):339-44. doi: 10.1002/cyto.990100314.