PMID- 27141791
OWN - NLM
STAT- MEDLINE
DCOM- 20160602
LR  - 20160504
IS  - 1003-0034 (Print)
IS  - 1003-0034 (Linking)
VI  - 29
IP  - 2
DP  - 2016 Feb
TI  - [Association of red blood cell damage with arachidonic acid].
PG  - 179-83
AB  - OBJECTIVE: To study the correlation between arachidonic acid (AA) and acute red 
      blood cells damage in rats, and to build a model with hidden blood loss in vivo, 
      and to explore the pathological mechenism of hidden blood loss. METHODS: A total 
      of 50 male adult Sprague-Dawley rats weighing (200 +/- 20) g were randomly divided 
      into five groups (n = 10): control group and four experimental groups. The rats 
      in the experimental groups were given 0.5 ml different concentrations of AA dilu- 
      ents, 5, 10, 20, 40 mmol/L respectively. The blood samples were collected from 
      orbital venous at the beginning and 24, 48, 72 hours after administration. Then 
      the changes of hemoglobin (Hb) ,red blood cell count (RBC), glutathione 
      peroxidase (GSH- PX) activity, total superoxide dismutase (T-SOD) activity and 
      hydrogen peroxide (H202) in the blood samples were tested. RESULTS: Significant 
      hidden blood loss occurred when the concentration was 10 mmol/L in the 
      experimental group, with the RBC and Hb sharply reduced in blood samples. The Hb 
      and RBC were reduced in all the experimental groups and control group at 24 hours 
      after administration, while in the experimental groups they changed more 
      obviously. The GSH-PX activity, T-SOD activity and H(2)O(2)were also significantly 
      reduced in all groups, and the changes showed significant differences. The Hb and 
      RBC were relatively stable in the control group and the experimental groups at 48 
      hours after administration; while GSH-PX activity, T-SOD activity and H(2)O(2)were 
      all significantly decreased, and the changes in the experimental groups were more 
      notable. CONCLUSION: Elevated levels of AA in the blood causes oxidative stress 
      in the red blood cells, leading to the damage of red blood cells and hemoglobin, 
      which is responsible for hidden blood loss.
FAU - Yuan, Tao
AU  - Yuan T
FAU - Zhao, Jian-ning
AU  - Zhao JN
FAU - Meng, Jia
AU  - Meng J
FAU - Cong, Yu
AU  - Cong Y
FAU - Chen, Shuang-shuang
AU  - Chen SS
FAU - Bao, Ni-rong
AU  - Bao NR
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Gu Shang
JT  - Zhongguo gu shang = China journal of orthopaedics and traumatology
JID - 9815790
RN  - 0 (Hemoglobins)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Arachidonic Acid/*toxicity
MH  - Erythrocytes/*drug effects/metabolism
MH  - Glutathione Peroxidase/blood
MH  - Hemoglobins/analysis
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Superoxide Dismutase/blood
EDAT- 2016/05/05 06:00
MHDA- 2016/06/03 06:00
CRDT- 2016/05/05 06:00
PHST- 2016/05/05 06:00 [entrez]
PHST- 2016/05/05 06:00 [pubmed]
PHST- 2016/06/03 06:00 [medline]
PST - ppublish
SO  - Zhongguo Gu Shang. 2016 Feb;29(2):179-83.