PMID- 27142006
OWN - NLM
STAT- MEDLINE
DCOM- 20171213
LR  - 20211204
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1423
DP  - 2016
TI  - In Vitro Generation of Human XCR1(+) Dendritic Cells from CD34(+) Hematopoietic 
      Progenitors.
PG  - 19-37
LID - 10.1007/978-1-4939-3606-9_2 [doi]
AB  - Dendritic cells (DCs) are a heterogeneous population of professional 
      antigen-presenting cells which play a key role in orchestrating immune defenses. 
      Most of the information gained on human DC biology was derived from studies 
      conducted with DCs generated in vitro from peripheral blood CD14(+) monocytes 
      (MoDCs) or from CD34(+) hematopoietic progenitors. Recent advances in the field 
      revealed that these types of in vitro-derived DCs strikingly differ from the DC 
      subsets that are naturally present in human lymphoid organs, in terms of global 
      gene expression, of specialization in the sensing of different types of danger 
      signals, and of the ability to polarize T lymphocytes toward different functions. 
      Major efforts are being made to better characterize the biology and the functions 
      of lymphoid organ-resident DC subsets in humans, as an essential step for 
      designing innovative DC-based vaccines against infections or cancers. However, 
      this line of research is hampered by the low frequency of certain DC subsets in 
      most tissues, their fragility, and the complexity of the procedures necessary for 
      their purification. Hence, there is a need for robust procedures allowing 
      large-scale in vitro generation of human DC subsets, under conditions allowing 
      their genetic or pharmacological manipulation, to decipher their functions and 
      their molecular regulation. Human CD141(+)CLEC9A(+)XCR1(+) DCs constitute a very 
      interesting DC subset for the design of immunotherapeutic treatments against 
      infections by intracellular pathogens or against cancer, because these cells 
      resemble mouse professional cross-presenting CD8alpha(+)Clec9a(+)Xcr1(+) DCs. Human 
      XCR1(+) DCs have indeed been reported by several teams to be more efficient than 
      other human DC subsets for cross-presentation, in particular of cell-associated 
      antigens but also of soluble antigens especially when delivered into late 
      endosomes or lysosomes. However, human XCR1(+) DCs are the rarest and perhaps the 
      most fragile of the human DC subsets and hence the most difficult to study ex 
      vivo. Here, we describe a protocol allowing simultaneous in vitro generation of 
      human MoDCs and XCR1(+) DCs, which will undoubtedly be extremely useful to better 
      characterize the functional specialization of human XCR1(+) DCs and to identify 
      its molecular bases.
FAU - Balan, Sreekumar
AU  - Balan S
AD  - Centre d'Immunologie de Marseille-Luminy, UNIV UM2, Aix Marseille Universite, 
      Parc Scientifique et Technologique de Luminy, 13288, Marseille, France.
AD  - Unite Mixte de Recherche 1104, Inserm, 13288, Marseille, France.
AD  - Unite Mixte de Recherche 7280, Centre National de la Recherche Scientifique 
      (CNRS), 13288, Marseille, France.
FAU - Dalod, Marc
AU  - Dalod M
AD  - Centre d'Immunologie de Marseille-Luminy, UNIV UM2, Aix Marseille Universite, 
      Parc Scientifique et Technologique de Luminy, 13288, Marseille, France. 
      dalod@ciml.univ-mrs.fr.
AD  - Unite Mixte de Recherche 1104, Inserm, 13288, Marseille, France. 
      dalod@ciml.univ-mrs.fr.
AD  - Unite Mixte de Recherche 7280, Centre National de la Recherche Scientifique 
      (CNRS), 13288, Marseille, France. dalod@ciml.univ-mrs.fr.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antigens, CD34)
RN  - 0 (Antigens, Surface)
RN  - 0 (CLEC9a protein, human)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Mitogen)
RN  - 0 (THBD protein, human)
RN  - 0 (Thrombomodulin)
RN  - 0 (XCR1 protein, human)
SB  - IM
MH  - Antigens, CD34/*metabolism
MH  - Antigens, Surface/metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Cross-Priming
MH  - Dendritic Cells/*cytology/immunology
MH  - Flow Cytometry
MH  - Hematopoietic Stem Cells/*cytology/immunology
MH  - Humans
MH  - In Vitro Techniques
MH  - Lectins, C-Type/metabolism
MH  - Monocytes/cytology/immunology
MH  - Receptors, G-Protein-Coupled/*metabolism
MH  - Receptors, Mitogen/metabolism
MH  - Thrombomodulin
OTO - NOTNLM
OT  - CD34+ hematopoietic stem cells
OT  - Cross-presentation
OT  - Differentiation
OT  - Human immune system
OT  - Monocyte-derived dendritic cells
OT  - XCR1+ dendritic cells
EDAT- 2016/05/05 06:00
MHDA- 2017/12/14 06:00
CRDT- 2016/05/05 06:00
PHST- 2016/05/05 06:00 [entrez]
PHST- 2016/05/05 06:00 [pubmed]
PHST- 2017/12/14 06:00 [medline]
AID - 10.1007/978-1-4939-3606-9_2 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2016;1423:19-37. doi: 10.1007/978-1-4939-3606-9_2.