PMID- 27142008
OWN - NLM
STAT- MEDLINE
DCOM- 20171213
LR  - 20180110
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1423
DP  - 2016
TI  - Isolation of Dendritic Cell Progenitor and Bone Marrow Progenitor Cells from 
      Mouse.
PG  - 53-9
LID - 10.1007/978-1-4939-3606-9_4 [doi]
AB  - Dendritic cells (DCs) comprise two major subsets, conventional DC (cDC) and 
      plasmacytoid DC (pDC) in the steady-state lymphoid organ. These cells have a 
      short half-life and therefore, require continuous generation from hematopoietic 
      stem cells and progenitor cells. Recently, we identified DC-restricted 
      progenitors called common DC progenitors (CDPs) in the bone marrow of mouse. The 
      CDPs can be isolated from mouse bone marrow based on the hematopoietic cytokine 
      receptors, such as Flt3 (Fms-related tyrosine kinase 3) (CD135), c-kit (CD117), 
      M-CSF (macrophage colony-stimulating factor) receptor (CD115), and IL-7 
      (interleukin-7) receptor-alpha (CD127). The CDPs comprise of two progenitors, 
      CD115(+) CDPs and CD115(-) CDPs, and give rise to only DC subsets in both in 
      vitro and in vivo. The former CDPs are the main source of cDC, while the later 
      CDPs are the main source of pDC in vivo. Here, we provide a protocol for the 
      isolation of dendritic cell progenitor and bone marrow progenitor cells from 
      mouse.
FAU - Onai, Nobuyuki
AU  - Onai N
AD  - Department of Biodefense, Medical Research Institute, Tokyo Medical and Dental 
      University, 1-5-45 Yushima, Bunkyo, Tokyo, 113-8510, Japan. 
      onai.bre@mri.tmd.ac.jp.
FAU - Ohteki, Toshiaki
AU  - Ohteki T
AD  - Department of Biodefense, Medical Research Institute, Tokyo Medical and Dental 
      University, 1-5-45 Yushima, Bunkyo, Tokyo, 113-8510, Japan.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Biomarkers)
RN  - 0 (Receptors, Cytokine)
RN  - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/cytology/immunology
MH  - Cell Separation/*methods
MH  - Dendritic Cells/*cytology/immunology
MH  - Hematopoietic Stem Cells/*cytology/immunology
MH  - Mice
MH  - Receptor, Macrophage Colony-Stimulating Factor/metabolism
MH  - Receptors, Cytokine/metabolism
OTO - NOTNLM
OT  - Common DC progenitors (CDPs)
OT  - Conventional dendritic cells (cDCs)
OT  - Cytokine receptor
OT  - Plasmacytoid DCs (pDCs)
EDAT- 2016/05/05 06:00
MHDA- 2017/12/14 06:00
CRDT- 2016/05/05 06:00
PHST- 2016/05/05 06:00 [entrez]
PHST- 2016/05/05 06:00 [pubmed]
PHST- 2017/12/14 06:00 [medline]
AID - 10.1007/978-1-4939-3606-9_4 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2016;1423:53-9. doi: 10.1007/978-1-4939-3606-9_4.