PMID- 27142012
OWN - NLM
STAT- MEDLINE
DCOM- 20171213
LR  - 20180110
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1423
DP  - 2016
TI  - Isolation of Human Skin Dendritic Cell Subsets.
PG  - 119-28
LID - 10.1007/978-1-4939-3606-9_8 [doi]
AB  - Dendritic cells (DCs) are specialized leukocytes with antigen-processing and 
      antigen-presenting functions. DCs can be divided into distinct subsets by 
      anatomical location, phenotype and function. In human, the two most accessible 
      tissues to study leukocytes are peripheral blood and skin. DCs are rare in human 
      peripheral blood (<1 % of mononuclear cells) and have a less mature phenotype 
      than their tissue counterparts (MacDonald et al., Blood. 100:4512-4520, 2002; 
      Haniffa et al., Immunity 37:60-73, 2012). In contrast, the skin covering an 
      average total surface area of 1.8 m(2) has approximately tenfold more DCs than 
      the average 5 L of total blood volume (Wang et al., J Invest Dermatol 
      134:965-974, 2014). DCs migrate spontaneously from skin explants cultured ex 
      vivo, which provide an easy method of cell isolation (Larsen et al., J Exp Med 
      172:1483-1493, 1990; Lenz et al., J Clin Invest 92:2587-2596, 1993; Nestle et 
      al., J Immunol 151:6535-6545, 1993). These factors led to the extensive use of 
      skin DCs as the "prototype" migratory DCs in human studies. In this chapter, we 
      detail the protocols to isolate DCs and resident macrophages from human skin. We 
      also provide a multiparameter flow cytometry gating strategy to identify human 
      skin DCs and to distinguish them from macrophages.
FAU - Gunawan, Merry
AU  - Gunawan M
AD  - Human DC Lab, Institute of Cellular Medicine, Newcastle University, Newcastle 
      upon Tyne, NE2 4HH, UK.
FAU - Jardine, Laura
AU  - Jardine L
AD  - Human DC Lab, Institute of Cellular Medicine, Newcastle University, Newcastle 
      upon Tyne, NE2 4HH, UK.
FAU - Haniffa, Muzlifah
AU  - Haniffa M
AD  - Human DC Lab, Institute of Cellular Medicine, Newcastle University, Newcastle 
      upon Tyne, NE2 4HH, UK. m.a.haniffa@ncl.ac.uk.
LA  - eng
GR  - WT088555/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Antigen Presentation
MH  - Cell Movement
MH  - Cell Separation/*methods
MH  - Dendritic Cells/*cytology/immunology
MH  - Flow Cytometry
MH  - Humans
MH  - Leukocytes, Mononuclear/cytology/immunology
MH  - Macrophages/cytology
MH  - Skin/*cytology/immunology
OTO - NOTNLM
OT  - Antigen-presenting cells
OT  - Dendritic cells
OT  - Macrophages
OT  - Mononuclear phagocytes
OT  - Skin
EDAT- 2016/05/05 06:00
MHDA- 2017/12/14 06:00
CRDT- 2016/05/05 06:00
PHST- 2016/05/05 06:00 [entrez]
PHST- 2016/05/05 06:00 [pubmed]
PHST- 2017/12/14 06:00 [medline]
AID - 10.1007/978-1-4939-3606-9_8 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2016;1423:119-28. doi: 10.1007/978-1-4939-3606-9_8.