PMID- 27142021
OWN - NLM
STAT- MEDLINE
DCOM- 20171213
LR  - 20180110
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1423
DP  - 2016
TI  - In Vivo Ablation of a Dendritic Cell Subset Expressing the Chemokine Receptor 
      XCR1.
PG  - 247-53
LID - 10.1007/978-1-4939-3606-9_17 [doi]
AB  - Dendritic cells (DCs) are one of the key populations controlling immune 
      responses. To establish a cell depletion system in vivo, human diphtheria toxin 
      (DT) receptor (DTR) is transduced to the mice in which DTR is expressed under the 
      control of a specific promoter. In these mice, DTR-expressing cells are inducibly 
      depleted after DT injection. Using this system, analysis of mouse models in which 
      DTR was expressed under the CD11c promoter has contributed to our knowledge of DC 
      biology by depleting CD11c(+) cells. Other mouse models to inducibly eliminate 
      specific DC subsets upon DT treatment have been also generated. Here, we describe 
      a new mouse model in which the XCR1(+) DC subset is inducibly and transiently 
      depleted in vivo.
FAU - Hemmi, Hiroaki
AU  - Hemmi H
AD  - Laboratory for Immune Regulation, WPI Immunology Frontier Research Center, Osaka 
      University, 3-1 Yamada-oka, Suita, Osaka, 565-0871, Japan.
AD  - Laboratory of Inflammatory Regulation, RIKEN Center for Integrative Medical 
      Sciences, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.
AD  - Department of Immunology, Institute of Advanced Medicine, Wakayama Medical 
      University, 811-1 Kimiidera, Wakayama, Wakayama, 641-8509, Japan.
FAU - Hoshino, Katsuaki
AU  - Hoshino K
AD  - Laboratory for Immune Regulation, WPI Immunology Frontier Research Center, Osaka 
      University, 3-1 Yamada-oka, Suita, Osaka, 565-0871, Japan.
AD  - Laboratory of Inflammatory Regulation, RIKEN Center for Integrative Medical 
      Sciences, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.
AD  - Department of Immunology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, 
      Miki-cho, Kita-gun, Kagawa, 761-0793, Japan.
FAU - Kaisho, Tsuneyasu
AU  - Kaisho T
AD  - Laboratory for Immune Regulation, WPI Immunology Frontier Research Center, Osaka 
      University, 3-1 Yamada-oka, Suita, Osaka, 565-0871, Japan. 
      tkaisho@wakayama-med.ac.jp.
AD  - Laboratory of Inflammatory Regulation, RIKEN Center for Integrative Medical 
      Sciences, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan. 
      tkaisho@wakayama-med.ac.jp.
AD  - Department of Immunology, Institute of Advanced Medicine, Wakayama Medical 
      University, 811-1 Kimiidera, Wakayama, Wakayama, 641-8509, Japan. 
      tkaisho@wakayama-med.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (CD11c Antigen)
RN  - 0 (Diphtheria Toxin)
RN  - 0 (HBEGF protein, human)
RN  - 0 (Heparin-binding EGF-like Growth Factor)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (XC chemokine receptor 1, mouse)
SB  - IM
MH  - Animals
MH  - CD11c Antigen/*genetics
MH  - Dendritic Cells/*immunology
MH  - Diphtheria Toxin/*pharmacology
MH  - Gene Knock-In Techniques
MH  - Heparin-binding EGF-like Growth Factor/genetics/*metabolism
MH  - Humans
MH  - Mice
MH  - Mice, Transgenic
MH  - Models, Animal
MH  - Promoter Regions, Genetic
MH  - Receptors, Chemokine/genetics/*metabolism
OTO - NOTNLM
OT  - Dendritic cell subset
OT  - Dendritic cells
OT  - Diphtheria toxin
OT  - Diphtheria toxin receptor
OT  - Inducible ablation of dendritic cell subsets
EDAT- 2016/05/05 06:00
MHDA- 2017/12/14 06:00
CRDT- 2016/05/05 06:00
PHST- 2016/05/05 06:00 [entrez]
PHST- 2016/05/05 06:00 [pubmed]
PHST- 2017/12/14 06:00 [medline]
AID - 10.1007/978-1-4939-3606-9_17 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2016;1423:247-53. doi: 10.1007/978-1-4939-3606-9_17.