PMID- 27142080
OWN - NLM
STAT- MEDLINE
DCOM- 20170313
LR  - 20201209
IS  - 1572-994X (Electronic)
IS  - 0920-8569 (Linking)
VI  - 52
IP  - 5
DP  - 2016 Oct
TI  - In vitro isolation and molecular identification of reptarenavirus in Malaysia.
PG  - 640-50
LID - 10.1007/s11262-016-1345-7 [doi]
AB  - Boid inclusion body disease (BIBD) is a viral disease of boids caused by 
      reptarenavirus. In this study, tissue from naturally infected boid snakes were 
      homogenized and propagated in African Monkey kidney (Vero) and rat embryonic 
      fibroblast (REF) cells. Virus replication was determined by the presence of 
      cytopathic effect, while viral morphology was observed using transmission 
      electron microscopy. Viral RNA was amplified using RT-PCR with primers specific 
      for the L-segment of reptarenavirus; similarly, quantification of viral 
      replication was done using qPCR at 24-144 h postinfection. Viral cytopathology 
      was characterized by cell rounding and detachment in both Vero and REF cells. The 
      viral morphology showed round-to-pleomorphic particles ranging from 105 to 150 nm 
      which had sand-like granules. Sanger sequencing identified four closely 
      associated reptarenavirus species from 15 (37.5 %) of the total samples tested, 
      and these were named as follows: reptarenavirus UPM-MY 01, 02, 03, and 04. These 
      isolates were phylogenetically closely related to the University Helsinki virus 
      (UHV), Boa Arenavirus NL (ROUTV; BAV), and unidentified reptarenavirus L20 
      (URAV-L20). Comparison of deduced amino acid sequences further confirmed 
      identities to L-protein of UHV, L-polymerase of BAV and RNA-dependent RNA 
      polymerase of URAV-L20. Viral replication in Vero cells increased steadily from 
      24 to 72 h and peaked at 144 h. This is the first study in South East Asia to 
      isolate and characterize reptarenavirus in boid snakes with BIBD.
FAU - Abba, Yusuf
AU  - Abba Y
AD  - Department of Veterinary Pathology and Microbiology, Faculty of Veterinary 
      Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. 
      yabbavet@gmail.com.
AD  - Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, 
      Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. 
      yabbavet@gmail.com.
FAU - Hassim, Hasliza
AU  - Hassim H
AD  - Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of 
      Maiduguri, PMB 1069, Maiduguri, 600233, Borno, Nigeria.
FAU - Hamzah, Hazilawati
AU  - Hamzah H
AD  - Department of Veterinary Pathology and Microbiology, Faculty of Veterinary 
      Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
FAU - Ibrahim, Omar Emad
AU  - Ibrahim OE
AD  - Department of Pathology, Faculty of Dentistry, Universiti Teknologi Mara, 47000, 
      Sungai Buloh, Selangor Darul Ehsan, Malaysia.
FAU - Ilyasu, Yusuf
AU  - Ilyasu Y
AD  - Department of Veterinary Pathology and Microbiology, Faculty of Veterinary 
      Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
AD  - Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, 
      Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
FAU - Bande, Faruku
AU  - Bande F
AD  - Department of Veterinary Pathology and Microbiology, Faculty of Veterinary 
      Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
FAU - Mohd Lila, Mohd Azmi
AU  - Mohd Lila MA
AD  - Department of Veterinary Pathology and Microbiology, Faculty of Veterinary 
      Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
FAU - Noordin, Mohamed Mustapha
AU  - Noordin MM
AD  - Department of Veterinary Pathology and Microbiology, Faculty of Veterinary 
      Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. 
      noordinmm@upm.edu.my.
LA  - eng
PT  - Journal Article
DEP - 20160503
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
RN  - EC 2.7.7.48 (RNA-Dependent RNA Polymerase)
SB  - IM
MH  - Animals
MH  - Arenavirus/*genetics/*isolation & purification
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Malaysia
MH  - Phylogeny
MH  - RNA, Viral/genetics
MH  - RNA-Dependent RNA Polymerase/genetics
MH  - Rats
MH  - Sequence Analysis, DNA/methods
MH  - Snakes/*virology
MH  - Vero Cells
MH  - Viral Proteins/genetics
MH  - Virus Replication/genetics
OTO - NOTNLM
OT  - Boid inclusion body disease
OT  - Cell culture
OT  - Electron microscopy
OT  - Reptarenavirus
OT  - Sanger sequencing
OT  - Viral isolation
EDAT- 2016/05/05 06:00
MHDA- 2017/03/14 06:00
CRDT- 2016/05/05 06:00
PHST- 2016/02/04 00:00 [received]
PHST- 2016/04/21 00:00 [accepted]
PHST- 2016/05/05 06:00 [entrez]
PHST- 2016/05/05 06:00 [pubmed]
PHST- 2017/03/14 06:00 [medline]
AID - 10.1007/s11262-016-1345-7 [pii]
AID - 10.1007/s11262-016-1345-7 [doi]
PST - ppublish
SO  - Virus Genes. 2016 Oct;52(5):640-50. doi: 10.1007/s11262-016-1345-7. Epub 2016 May 
      3.