PMID- 2714371
OWN - NLM
STAT- MEDLINE
DCOM- 19890615
LR  - 20190624
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 172
IP  - 1
DP  - 1989 Mar 7
TI  - Acute inactivation of tryptophan hydroxylase by amphetamine analogs involves the 
      oxidation of sulfhydryl sites.
PG  - 93-7
AB  - The activity of rat hippocampal tryptophan hydroxylase was reduced from 30-60% 3 
      h after the administration of a 10-15 mg/kg dose of either fenfluramine, 
      methamphetamine or 3,4-methylenedioxymethamphetamine (MDMA). Tryptophan 
      hydroxylase inactivated by these drug treatments could be reconstituted by a 
      prolonged anaerobic incubation in the presence of 5 mM dithiothreitol and 50 
      microM Fe2+. Drug-inactivated enzyme obtained from rats killed 18 h after 
      multiple doses of either D(+)- or L(-)-MDMA could not be similarly restored. 
      These observations suggest that the rapid decrease in central tryptophan 
      hydroxylase activity induced by amphetamine analogs results from the reversible 
      oxidation of a sulfhydryl site(s) within the enzyme molecule, whereas the 
      irreversible decrease in enzymatic activity measured 18 h after multiple-dose 
      MDMA treatment may reflect serotonergic toxicity.
FAU - Stone, D M
AU  - Stone DM
AD  - Department of Pharmacology and Toxicology, University of Utah, Salt Lake City 
      84112.
FAU - Johnson, M
AU  - Johnson M
FAU - Hanson, G R
AU  - Hanson GR
FAU - Gibb, J W
AU  - Gibb JW
LA  - eng
GR  - DA 00869/DA/NIDA NIH HHS/United States
GR  - DA 04221/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Amphetamines)
RN  - 0 (Sulfhydryl Compounds)
RN  - E1UOL152H7 (Iron)
RN  - EC 1.14.16.4 (Tryptophan Hydroxylase)
RN  - T8ID5YZU6Y (Dithiothreitol)
SB  - IM
MH  - Amphetamines/*pharmacology
MH  - Anaerobiosis
MH  - Animals
MH  - Dithiothreitol/pharmacology
MH  - Enzyme Activation/drug effects
MH  - Hippocampus/enzymology
MH  - Iron/pharmacology
MH  - Male
MH  - Oxidation-Reduction
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Sulfhydryl Compounds/*analysis
MH  - Tryptophan Hydroxylase/*antagonists & inhibitors
EDAT- 1989/03/07 00:00
MHDA- 1989/03/07 00:01
CRDT- 1989/03/07 00:00
PHST- 1989/03/07 00:00 [pubmed]
PHST- 1989/03/07 00:01 [medline]
PHST- 1989/03/07 00:00 [entrez]
AID - 10.1016/0922-4106(89)90048-5 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 1989 Mar 7;172(1):93-7. doi: 10.1016/0922-4106(89)90048-5.