PMID- 27147644
OWN - NLM
STAT- MEDLINE
DCOM- 20171129
LR  - 20180205
IS  - 1748-605X (Electronic)
IS  - 1748-6041 (Linking)
VI  - 11
IP  - 3
DP  - 2016 May 5
TI  - The migration and differentiation of hUC-MSCs(CXCR4/GFP) encapsulated in 
      BDNF/chitosan scaffolds for brain tissue engineering.
PG  - 035004
LID - 10.1088/1748-6041/11/3/035004 [doi]
AB  - We previously developed a biomaterial scaffold that could effectively provide 
      seed cells to a lesion cavity resulting from traumatic brain injury. However, we 
      subsequently found that few transplanted human umbilical cord mesenchymal stem 
      cells (hUC-MSCs) are able to migrate from the scaffold to the lesion boundary. 
      Stromal derived-cell factor-1alpha and its receptor chemokine (C-X-C motif) receptor 
      (CXCR)4 are chemotactic factors that control cell migration and stem cell 
      recruitment to target areas. Given the low expression level of CXCR4 on the 
      hUC-MSC membrane, lentiviral vectors were used to generate hUC-MSCs stably 
      expressing CXCR4 fused to green fluorescent protein (GFP) (hUC-MSCs(CXCR4/GFP)). 
      We constructed a scaffold in which recombinant human brain-derived neurotrophic 
      factor (BDNF) was linked to chitosan scaffolds with the crosslinking agent 
      genipin (CGB scaffold). The scaffold containing hUC-MSCs(CXCR4/GFP) was 
      transplanted into the lesion cavity of a rat brain, providing exogenous hUC-MSCs 
      to both lesion boundary and cavity. These results demonstrate a novel strategy 
      for inducing tissue regeneration after traumatic brain injury.
FAU - Huang, Chuanjun
AU  - Huang C
AD  - Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, 
      People's Republic of China.
FAU - Zhao, Longxiang
AU  - Zhao L
FAU - Gu, Jun
AU  - Gu J
FAU - Nie, Dekang
AU  - Nie D
FAU - Chen, Yinan
AU  - Chen Y
FAU - Zuo, Hao
AU  - Zuo H
FAU - Huan, Wei
AU  - Huan W
FAU - Shi, Jinlong
AU  - Shi J
FAU - Chen, Jian
AU  - Chen J
FAU - Shi, Wei
AU  - Shi W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160505
PL  - England
TA  - Biomed Mater
JT  - Biomedical materials (Bristol, England)
JID - 101285195
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (CXCR4 protein, human)
RN  - 0 (Cxcr4 protein, rat)
RN  - 0 (Iridoids)
RN  - 0 (Receptors, CXCR4)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 9012-76-4 (Chitosan)
RN  - A3V2NE52YG (genipin)
SB  - IM
MH  - Animals
MH  - Brain/metabolism
MH  - Brain Injuries, Traumatic/therapy
MH  - Brain-Derived Neurotrophic Factor/chemistry
MH  - Cell Adhesion
MH  - *Cell Differentiation
MH  - *Cell Movement
MH  - Cell Proliferation
MH  - Chitosan/chemistry
MH  - Gene Expression Profiling
MH  - Green Fluorescent Proteins/chemistry
MH  - Humans
MH  - Iridoids/chemistry
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, CXCR4/metabolism
MH  - Tissue Engineering/*methods
MH  - *Tissue Scaffolds
EDAT- 2016/05/06 06:00
MHDA- 2017/12/01 06:00
CRDT- 2016/05/06 06:00
PHST- 2016/05/06 06:00 [entrez]
PHST- 2016/05/06 06:00 [pubmed]
PHST- 2017/12/01 06:00 [medline]
AID - 10.1088/1748-6041/11/3/035004 [doi]
PST - epublish
SO  - Biomed Mater. 2016 May 5;11(3):035004. doi: 10.1088/1748-6041/11/3/035004.