PMID- 27148410
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160505
LR  - 20220316
IS  - 1758-5996 (Print)
IS  - 1758-5996 (Electronic)
IS  - 1758-5996 (Linking)
VI  - 8
DP  - 2016
TI  - Oxidative DNA damage and oxidized low density lipoprotein in Type II diabetes 
      mellitus among patients with Helicobacter pylori infection.
PG  - 34
LID - 10.1186/s13098-016-0149-1 [doi]
LID - 34
AB  - BACKGROUND: Helicobacter pylori (H. pylori) infection is reported to be 
      associated with various extragastrointestinal conditions such as insulin 
      resistance, diabetes mellitus and metabolic syndrome. H. pylori infection and 
      type 2 diabetes mellitus (T2DM) are associated with oxidative stress, this 
      cross-relation between H. pylori induced infection in T2DM and oxidative damage 
      is still debated. Thus, the question arises whether an increase in the serum 
      level of 8-OHdG and Ox-LDL will occurs in patients with T2DM infected H. pylori; 
      this will be through determination and compare frequency of H. pylori infection 
      in T2DM and non-diabetic patients. METHODS: 100 patients presented with history 
      of epigastric discomfort for more than 1 month; 50 patients with T2DM and 50 
      non-diabetics. Anti-H. pylori IgG using ELISA, fasting and postprandial glucose 
      level, glycated hemoglobin (HbA1c) and body mass index (BMI) was calculated. 
      Serum 8-OHdG and Ox-LDL was measured using ELISA for the 100 patients and 50 
      control subject. RESULTS: Rates of H. pylori infection of T2DM and non-diabetic 
      were 66 and 58 %, respectively, (p = 0.001). H. pylori IgG antibody was not 
      correlated with HbA1c either in T2DM (p = 0.06) or non-diabetic (p = 0.25). Serum 
      8-OHdG level in T2DM with positive H. pylori infection showed a significant 
      difference compared to non-diabetics with positive H. pylori infection 
      (p = 0.001) and higher than that in T2DM with negative H. pylori. A correlation 
      between 8-OHdG concentration and HbA1c in T2DM patients infected with H. pylori 
      was observed (r = 0.39, p = 0.02). Serum Ox-LDL level in T2DM with positive H. 
      pylori infection showed a significant difference compared to diabetics with both 
      negative H. pylori infection and in non-diabetics with positive H. pylori 
      infection (p = 0.001). CONCLUSIONS: Increased levels of oxidative DNA damage 
      (8-OHdG) and Ox-LDL suggest the mechanistic link between H. pylori infection 
      combined with diabetes and increased generation of ROS and could play as an 
      important image for high risk to atherosclerosis.
FAU - Nasif, Wesam Ahmed
AU  - Nasif WA
AD  - Biochemistry Department, Faculty of Medicine, Umm Al-Qura University, Makkah, 
      Kingdom of Saudi Arabia ; Molecular Biology Department, Genetic Engineering and 
      Biotechnology Research Institute, Sadat City University, Sadat City, Egypt.
FAU - Mukhtar, Mohammed Hasan
AU  - Mukhtar MH
AD  - Biochemistry Department, Faculty of Medicine, Umm Al-Qura University, Makkah, 
      Kingdom of Saudi Arabia.
FAU - Nour Eldein, Mohammed Mahmoud
AU  - Nour Eldein MM
AD  - Biochemistry Department, Faculty of Medicine, Umm Al-Qura University, Makkah, 
      Kingdom of Saudi Arabia ; Oncology Diagnostic Unit, Faculty of Medicine, Ain 
      Shams University, Cairo, Egypt.
FAU - Ashgar, Sami Sadagah
AU  - Ashgar SS
AD  - Microbiology Department, Faculty of Medicine, Umm Al-Qura University, Makkah, 
      Kingdom of Saudi Arabia.
LA  - eng
PT  - Journal Article
DEP - 20160503
PL  - England
TA  - Diabetol Metab Syndr
JT  - Diabetology & metabolic syndrome
JID - 101488958
PMC - PMC4855822
OTO - NOTNLM
OT  - 8-Hydroxydeoxyguanosine
OT  - Helicobacter pylori
OT  - Oxidized low density lipoprotein
OT  - Type 2 diabetes mellitus
EDAT- 2016/05/06 06:00
MHDA- 2016/05/06 06:01
PMCR- 2016/05/03
CRDT- 2016/05/06 06:00
PHST- 2015/10/13 00:00 [received]
PHST- 2016/04/18 00:00 [accepted]
PHST- 2016/05/06 06:00 [entrez]
PHST- 2016/05/06 06:00 [pubmed]
PHST- 2016/05/06 06:01 [medline]
PHST- 2016/05/03 00:00 [pmc-release]
AID - 149 [pii]
AID - 10.1186/s13098-016-0149-1 [doi]
PST - epublish
SO  - Diabetol Metab Syndr. 2016 May 3;8:34. doi: 10.1186/s13098-016-0149-1. 
      eCollection 2016.