PMID- 27150991
OWN - NLM
STAT- MEDLINE
DCOM- 20160915
LR  - 20181202
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 24
IP  - 2
DP  - 2016 Apr
TI  - [Application of Combined Detection of Fusion Gene and BIOMED-2 Standardized Ig 
      Gene Rearrangement System in Childhood B-cell Acute Lymphoblastic Leukemia].
PG  - 352-7
LID - 10.7534/j.issn.1009-2137.2016.02.009 [doi]
AB  - OBJECTIVE: To explore the application of combined detection of fusion gene and 
      BIOMED-2 standardized immunoglobulin (Ig) gene rearrangement system in diagnosis 
      and treatment of children with acute lymphoblastic leukemia (ALL). METHODS: 
      Multiplex-PCR amplifications and RQ-PCR of RNA/DNA were performed using ALL 
      fusion gene detection kit and BIOMED-2 primer. The Ig gene rearrangements were 
      analyzed by using PCR fragment analysis system. RESULTS: Out of 251 children with 
      B-ALL, 77 cases were TEL-AML1(+) , 28 cases were E2A-PBX1(+) , 10 cases were 
      MLL-AF4(+) , 11 cases were BCR-ABL(+) , the total positive rate was 50.2%, 82.5% 
      showed IgH VH-JH rearrangement, 53.4% showed IgK rearrangement. The positive rate 
      of combined detection of fusion gene and gene rearrangement was 99%. E2A-PBX1(+) 
      and MLL-AF4(+) with IgK(+) gene rearrangement group was compared with negative 
      control group, the difference was statistically significant (P < 0.001 or P = 
      0.005); 105 ALL fusion gene positive cases had been detected by fluorescence in 
      situ hybridization (FISH) simultaneously, the accordance rate of fusion gene and 
      FISH was more than 94%. CONCLUSION: The combined detection of ALL fusion gene and 
      BIOMED-2 standardized clonality analysis system can improve the positive detected 
      rate of B-ALL dramatically, and make the grouping of disease prognosis more 
      accurately; this combined detection is a more faster and sensitive method than 
      FISH.
FAU - Ai, Xiao-Fei
AU  - Ai XF
AD  - Center of Pathology, Institute of Hematology & Blood Disease Hospital, Chinese 
      Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, 
      China.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Center of Pathology, Institute of Hematology & Blood Disease Hospital, Chinese 
      Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, 
      China.
FAU - Shi, Xin-Rong
AU  - Shi XR
AD  - Department of Laboratorial Examination, Tianjin Third Central Hospital, Tianjin 
      300170, China.
FAU - Zheng, Ying-Chun
AU  - Zheng YC
AD  - Center of Pathology, Institute of Hematology & Blood Disease Hospital, Chinese 
      Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, 
      China.
FAU - Zhang, Li
AU  - Zhang L
AD  - Center of Pathology, Institute of Hematology & Blood Disease Hospital, Chinese 
      Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, 
      China.
FAU - Wang, Xiao-Jing
AU  - Wang XJ
AD  - Center of Pathology, Institute of Hematology & Blood Disease Hospital, Chinese 
      Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, 
      China.
FAU - Li, Qing-Hua
AU  - Li QH
AD  - Center of Pathology, Institute of Hematology & Blood Disease Hospital, Chinese 
      Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, 
      China. E-mail: qhli@ihcams.ac.cn.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
RN  - 0 (DNA Primers)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (TEL-AML1 fusion protein)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Child
MH  - Core Binding Factor Alpha 2 Subunit/*genetics
MH  - DNA Primers
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Multiplex Polymerase Chain Reaction
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/diagnosis/*genetics
MH  - *V(D)J Recombination
EDAT- 2016/05/07 06:00
MHDA- 2016/09/16 06:00
CRDT- 2016/05/07 06:00
PHST- 2016/05/07 06:00 [entrez]
PHST- 2016/05/07 06:00 [pubmed]
PHST- 2016/09/16 06:00 [medline]
AID - 1009-2137(2016)02-0352-06 [pii]
AID - 10.7534/j.issn.1009-2137.2016.02.009 [doi]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2016 Apr;24(2):352-7. doi: 
      10.7534/j.issn.1009-2137.2016.02.009.