PMID- 27152106
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160506
LR  - 20201001
IS  - 1611-2156 (Print)
IS  - 1611-2156 (Electronic)
IS  - 1611-2156 (Linking)
VI  - 14
DP  - 2015
TI  - Ultrasonic-assisted enzymatic extraction of silymarin from the Silybum marianum 
      seed shell and evaluation of its antioxidant activity in vitro.
PG  - 861-74
LID - 10.17179/excli2015-257 [doi]
AB  - This study revealed the optimal conditions for the Ultrasonic-Assisted Enzymatic 
      Extraction (UAEE) of silymarin, and include: the concentration of ethanol, 50 %; 
      enzyme concentration, 30 U/mg; liquid-solid ratio, 6:1; an extraction time of 120 
      min; and the ultrasonic power at 180 W. The extraction rate was 7.86 %, which is 
      higher, by 74.67 %, than that of the silymarin extract from the Silybum marianum 
      meal prepared by a distinct approach. SEM micrographs of the inner and outer 
      surfaces of the Silybum marianum shell obtained by variant extractions 
      demonstrated that the extraction of silymarin required the destruction of cell 
      walls. The results suggest that UAEE is a promising alternative for the 
      extraction of silymarin. The antioxidant activities of the silymarin were 
      evaluated in vitro by its capabilities to scavenger the DPPH, hydroxyl and 
      superoxide free radicals, as well as by its tyrosinase inhibitory activity. The 
      results showed that silymarin has significant antioxidant activity, thus it can 
      be used as a functional food material against oxidative stress. We believe that 
      the knowledge gained from this study should contribute to the further development 
      and application of this resource.
FAU - Zhao, Fei
AU  - Zhao F
AD  - College of Food Science, Shenyang Agricultural University, Shenyang 110866, 
      China.
FAU - Li, XinHua
AU  - Li X
AD  - College of Food Science, Shenyang Agricultural University, Shenyang 110866, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20150727
PL  - Germany
TA  - EXCLI J
JT  - EXCLI journal
JID - 101299402
PMC - PMC4849107
OTO - NOTNLM
OT  - Box-Behnken design
OT  - SEM
OT  - Silybum marianum
OT  - Silymarin
OT  - antioxidant activity
OT  - ultrasonic assisted enzymatic extraction
EDAT- 2015/01/01 00:00
MHDA- 2015/01/01 00:01
PMCR- 2015/07/27
CRDT- 2016/05/07 06:00
PHST- 2015/04/04 00:00 [received]
PHST- 2015/06/07 00:00 [accepted]
PHST- 2016/05/07 06:00 [entrez]
PHST- 2015/01/01 00:00 [pubmed]
PHST- 2015/01/01 00:01 [medline]
PHST- 2015/07/27 00:00 [pmc-release]
AID - 2015-257 [pii]
AID - Doc861 [pii]
AID - 10.17179/excli2015-257 [doi]
PST - epublish
SO  - EXCLI J. 2015 Jul 27;14:861-74. doi: 10.17179/excli2015-257. eCollection 2015.