PMID- 27154496
OWN - NLM
STAT- MEDLINE
DCOM- 20170131
LR  - 20170131
IS  - 1872-7786 (Electronic)
IS  - 0009-2797 (Linking)
VI  - 254
DP  - 2016 Jul 25
TI  - Red paprika (Capsicum annuum L.) and its main carotenoids, capsanthin and 
      beta-carotene, prevent hydrogen peroxide-induced inhibition of gap-junction 
      intercellular communication.
PG  - 146-55
LID - S0009-2797(16)30172-7 [pii]
LID - 10.1016/j.cbi.2016.05.004 [doi]
AB  - This study was conducted to investigate the protective effect of red paprika 
      extract (RPE) and its main carotenoids, namely, capsanthin (CST) and beta-carotene 
      (BCT), on the H2O2-induced inhibition of gap-junction intercellular communication 
      (GJIC) in WB-F344 rat liver epithelial cells (WB cells). We found that 
      pre-treatment with RPE, CST and BCT protected WB cells from H2O2-induced 
      inhibition of GJIC. RPE, CST and BCT not only recovered connexin 43 (Cx43) mRNA 
      expression but also prevented phosphorylation of Cx43 protein by H2O2 treatment. 
      RPE attenuated the phosphorylation of ERK, p38 and JNK, whereas pre-treatment 
      with CST and BCT only attenuated the phosphorylation of ERK and p38 and did not 
      affect JNK in H2O2-treated WB cells. RPE, CST and BCT significantly suppressed 
      the formation of reactive oxygen species (ROS) in H2O2-treated cells compared to 
      untreated WB cells. These results suggest that dietary intake of red paprika 
      might be helpful for lowering the risk of diseases caused by oxidative stress.
CI  - Copyright (c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Kim, Ji-Sun
AU  - Kim JS
AD  - Department of Food and Nutrition in Human Ecology, College of Natural Sciences, 
      Korea National Open University, 86, Daehak-ro, Jongno-gu, Seoul, 03078, Republic 
      of Korea.
FAU - Lee, Woo-Moon
AU  - Lee WM
AD  - Vegetable Research Division, National Institute of Horticultural & Herbal 
      Science, RDA, Wanju, 55365, Republic of Korea.
FAU - Rhee, Han Cheol
AU  - Rhee HC
AD  - Protected Horticulture Research Institute, National Institute of Horticultural & 
      Herbal Science, RDA, Haman, 52054, Republic of Korea.
FAU - Kim, Suna
AU  - Kim S
AD  - Department of Food and Nutrition in Human Ecology, College of Natural Sciences, 
      Korea National Open University, 86, Daehak-ro, Jongno-gu, Seoul, 03078, Republic 
      of Korea. Electronic address: ksuna7@knou.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20160503
PL  - Ireland
TA  - Chem Biol Interact
JT  - Chemico-biological interactions
JID - 0227276
RN  - 0 (Connexin 43)
RN  - 0 (Plant Extracts)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Xanthophylls)
RN  - 01YAE03M7J (beta Carotene)
RN  - 420NY1J57N (capsanthin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Capsicum/*chemistry/metabolism
MH  - Cell Communication/*drug effects
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Connexin 43/genetics/metabolism
MH  - Epithelial Cells/cytology/drug effects/metabolism
MH  - Gap Junctions/drug effects/metabolism
MH  - Hydrogen Peroxide/toxicity
MH  - JNK Mitogen-Activated Protein Kinases/metabolism
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Oxidative Stress/drug effects
MH  - Phosphorylation/drug effects
MH  - Plant Extracts/chemistry/pharmacology
MH  - Rats
MH  - Rats, Inbred F344
MH  - Reactive Oxygen Species/metabolism
MH  - Xanthophylls/pharmacology
MH  - beta Carotene/*pharmacology
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
OTO - NOTNLM
OT  - Capsanthin
OT  - Connexin 43
OT  - Gap junctional intercellular communication
OT  - Reactive oxygen species
OT  - Red paprika
OT  - beta-Carotene
EDAT- 2016/05/08 06:00
MHDA- 2017/02/01 06:00
CRDT- 2016/05/08 06:00
PHST- 2015/10/30 00:00 [received]
PHST- 2016/04/20 00:00 [revised]
PHST- 2016/05/02 00:00 [accepted]
PHST- 2016/05/08 06:00 [entrez]
PHST- 2016/05/08 06:00 [pubmed]
PHST- 2017/02/01 06:00 [medline]
AID - S0009-2797(16)30172-7 [pii]
AID - 10.1016/j.cbi.2016.05.004 [doi]
PST - ppublish
SO  - Chem Biol Interact. 2016 Jul 25;254:146-55. doi: 10.1016/j.cbi.2016.05.004. Epub 
      2016 May 3.