PMID- 27155390
OWN - NLM
STAT- MEDLINE
DCOM- 20171003
LR  - 20240109
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1857
IP  - 9
DP  - 2016 Sep
TI  - Involvement of the Lhcx protein Fcp6 of the diatom Cyclotella meneghiniana in the 
      macro-organisation and structural flexibility of thylakoid membranes.
PG  - 1373-1379
LID - S0005-2728(16)30387-5 [pii]
LID - 10.1016/j.bbabio.2016.04.288 [doi]
AB  - Diatoms possess special light-harvesting proteins involved in the photoprotection 
      mechanism called non-photochemical quenching (NPQ). These Lhcx proteins were 
      shown to be subunits of trimeric fucoxanthin-chlorophyll complexes (FCPa) in 
      centric diatoms, but their mode of action is still unclear. Here we investigated 
      the influence of Fcp6, an orthologue to Lhcx1 of Thalassiosira pseudonana in the 
      diatom Cyclotella meneghiniana, by reducing its amount using an antisense 
      approach. Whereas the pigment interactions inside FCPa were not influenced by the 
      presence or absence of Fcp6, as demonstrated by unaltered spectra of circular 
      dichroism, changes could be observed on the level of thylakoids and cells in the 
      mutants compared to WT. This fits to recent models of NPQ in diatoms, where FCP 
      aggregation or supramolecular reorganisation is thought to be a major feature. 
      Thus, Fcp6 (Lhcx1) appears to alter pigment-pigment interactions inside the 
      aggregates, but not inside (un-aggregated) FCPa itself.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Ghazaryan, Artur
AU  - Ghazaryan A
AD  - Institute of Molecular Biosciences, Goethe University Frankfurt, Max von Laue 
      Str. 9, 60438 Frankfurt, Germany.
FAU - Akhtar, Parveen
AU  - Akhtar P
AD  - Hungarian Academy of Sciences, Biological Research Centre, Temesvari krt. 62, 
      6726 Szeged, Hungary.
FAU - Garab, Gyozo
AU  - Garab G
AD  - Hungarian Academy of Sciences, Biological Research Centre, Temesvari krt. 62, 
      6726 Szeged, Hungary.
FAU - Lambrev, Petar H
AU  - Lambrev PH
AD  - Hungarian Academy of Sciences, Biological Research Centre, Temesvari krt. 62, 
      6726 Szeged, Hungary.
FAU - Buchel, Claudia
AU  - Buchel C
AD  - Institute of Molecular Biosciences, Goethe University Frankfurt, Max von Laue 
      Str. 9, 60438 Frankfurt, Germany. Electronic address: 
      C.Buechel@bio.uni-frankfurt.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160504
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Light-Harvesting Protein Complexes)
RN  - 0 (Protein Aggregates)
RN  - 0 (fucoxanthin-, chlorophyll a-c-containing protein)
SB  - IM
MH  - Circular Dichroism
MH  - Diatoms/*metabolism
MH  - Light-Harvesting Protein Complexes/chemistry/*physiology
MH  - Protein Aggregates
MH  - Thylakoids/*chemistry
OTO - NOTNLM
OT  - Anisotropic circular dichroism
OT  - FCPa
OT  - LHCSR
OT  - Lhcx1
OT  - Non-photochemical quenching
EDAT- 2016/05/08 06:00
MHDA- 2017/10/04 06:00
CRDT- 2016/05/08 06:00
PHST- 2016/03/15 00:00 [received]
PHST- 2016/04/18 00:00 [revised]
PHST- 2016/04/26 00:00 [accepted]
PHST- 2016/05/08 06:00 [entrez]
PHST- 2016/05/08 06:00 [pubmed]
PHST- 2017/10/04 06:00 [medline]
AID - S0005-2728(16)30387-5 [pii]
AID - 10.1016/j.bbabio.2016.04.288 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2016 Sep;1857(9):1373-1379. doi: 
      10.1016/j.bbabio.2016.04.288. Epub 2016 May 4.